1. Fruitbreedomics, Angers (F) 28.03.11 The apple genome sequence: summary, updates and developments WP6: Development of marker/gene arrays for high-throughput genotyping Riccardo Velasco, FEM-IASMA (I)
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5. Genetic structure in Malus 27 SSRs on 150 accessions M. domestica M. sieversii M. sylvestris Displayed only diploids Cluster analysis by DICE/UPGMA STRUCTURE (K =2-8) Micheletti, Salvi et al, in preparation. 2 3 4 5 6 7 8 K = Old cultivars M. sieversii M. sylvestris Modern cultivars
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Notes de l'éditeur
1) The crude estimated of SNP transferability = the heterozygous state of a SNP from one cultivar to another, within M. domestica, was Tsnp = 14.8%, derived from crude amplicon re-sequencing. However, a simple selection for SNP with MAF > 0.1 enabled to obtain Tsnp = 30.2% 2) a similar results was obtained based on a set of 209 SNPs Golden D SNPs selected also to be polymorphich across apple EST databases. In this case Tsnp = 40.9% etc.
![SNP genotyping at FEM-IASMA ,[object Object],[object Object],[object Object],[object Object],SNP mining in the Golden D genome sequence Coding regions of Golden Delicious = 1/227 bp 1/52 a whole genome estimate, a genic region estimate is less polymorphic Freedom Evo LiHa with Tecan 96 multi-channel pipetting head (TeMO) Platform](data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7)