5. LE 6-2
10 m
Human height
1m
Length of some
nerve and
muscle cells
Unaided eye
0.1 m
Chicken egg
1 cm
Frog egg
1 mm
Measurements
Light microscope
1 centimeter (cm) = 10–2 meter (m) = 0.4 inch 100 µm
1 millimeter (mm) = 10–3 m
1 micrometer (µm) = 10–3 mm = 10–6 m Most plant and
1 nanometer (nm) = 10–3 µm = 10–9 m animal cells
10 µm
Nucleus
Most bacteria
Mitochondrion
Electron microscope
1 µm
Smallest bacteria
100 nm
Viruses
Ribosomes
10 nm
Proteins
Lipids
1 nm
Small molecules
0.1 nm Atoms
7. LE 6-4
Electron Microscopes
1 µm
Cilia
Used to study subcellular
structures
Scanning electron microscopes
(SEMs) focus a beam of electrons
onto the surface of a specimen,
providing images that look 3D
Longitudinal Cross section
section of of cilium
cilium 1 µm
Transmission electron
microscopes (TEMs) focus a
beam of electrons through a
specimen
TEMs are used mainly to study
the internal ultrastructure of cells
11. The two types of cells
Prokaryotic vs. Eukaryotic Cells
Nucleus- no yes
Membrane
Bound organelles no yes
Plasma membrane yes yes
Cytosol yes yes
Chromosomes yes yes
Ribosomes yes yes
Domain Bacteria, Archea protist ,fungi, plant
animal
12. LE 6-6
Pili
Nucleoid
Ribosomes
Plasma
membrane
Cell wall
Bacterial
chromosome Capsule
0.5 µm
Flagella
A typical A thin section through the
rod-shaped bacterium Bacillus
bacterium coagulans (TEM)
13. LE 6-7
Surface area increases while
Total volume remains constant
Larger organisms do not have
larger cells- simple more cells
5
1
1
Total surface area
(height x width x 6 150 750
number of sides x
number of boxes)
Total volume
(height x width x length 1 125 125
X number of boxes)
Surface-to-volume
ratio
6 1.2 6
(surface area ÷ volume)
14. The boundary of every cell – Plasma membrane
Outside of cell
Carbohydrate side chain
Hydrophilic
region
Inside of cell 0.1 µm
Hydrophobic
region
Hydrophilic Phospholipid Proteins
region
TEM of a plasma membrane Structure of the plasma membrane
Function - selective barrier that allows sufficient passage
of oxygen, nutrients, and waste
Structure - double layer of phospholipids
15. A Panoramic View of the Eukaryotic Cell
ENDOPLASMIC RETICULUM (ER
Nuclear envelope
Flagellum Rough ER Smooth ER
Nucleolus NUCLEUS
Chromatin
In animal
cells but not
Centrosome
Plasma membrane plant cells:
Lysosomes
CYTOSKELETON
Centrioles
Microfilaments
Intermediate filaments
Flagella (in
Microtubules
some plant
Ribosomes:
sperm)
Microvilli
Golgi apparatus
Peroxisom
e
Mitochondrion
Lysosome
16. Nuclear A Panoramic View of the Eukaryotic Cell
envelope Rough
NUCLEUS Nucleolus endoplasmic
reticulum
Chromatin
Smooth
Centrosome endoplasmic
reticulum
Ribosomes
(small brown dots)
Central vacuole
Golgi
apparatus
Microfilaments
Intermediate CYTOSKELETON
filaments
Microtubules In plant cells but not
animal cells:
Mitochondrion
Chloroplasts
Peroxisome
Plasma Chloroplast
membrane Central vacuole and
Cell wall tonoplast
Plasmodesmata
Wall of adjacent cell
Cell wall
Plasmodesmata
LMs can magnify effectively to about 1,000 times the size of the actual specimen. Various techniques enhance contrast and enable cell components to be stained or labeled. Most subcellular structures, or organelles, are too small to be resolved by a LM.
Cell fractionation takes cells apart and separates the major organelles from one another. Ultracentrifuges fractionate cells into their component parts. Cell fractionation enables scientists to determine the functions of organelles. Isolating Organelles by Cell Fractionation. Cell fractionation enables the researcher to prepare specific components of cells in bulk quantity to study their composition and functions. By following this approach, biologists have been able to assign various functions of the cell to the different organelles, a task that would be far more difficult with intact cells. For example, one cellular fraction collected by centrifugation has enzymes that function in the metabolic process known as cellular respiration. The electron microscope reveals this fraction to be very rich in the organelles called mitochondria. This evidence helped cell biologists determine that mitochondria are the sites of cellular respiration. Cytology and biochemistry complement each other in correlating cellular structure and function.