2. Make sure that you check Moodle daily. You will have a pre-lab quiz every week posted on Moodle. All of your worksheets also will be posted on Moodle.

4. Bioscience General rules Please turn off your cell phone and put it away. Cells phones are not to be used in lab unless otherwise noted by the instructor. There are times that you can use the cell phone to take photos during a particular lab. Ask before using a cell phone as a camera. There is no food or drink allowed in the lab. That includes water bottles and chewing gum. RULES OF THE LAB

5. Bioscience General rules Do not be late – If you come to lab late, you will not be able to make up any missed work or quizzes. Do not cheat – Any plagiarism will result in an automatic zero for the lab for all parties involved. Wear proper attire (closed toe shoes, long pants, tie long hair back, no tank tops). Wear goggles and gloves when instructed

7. Bioscience Safety

8. Basic lab safety rules 1. Locate all safety equipment –emergency shower, eye wash stations, emergency electric and gas shutoff valves, first aid supplies 2. Identify all emergency evacuation routes. 3. Closed shoes are required. No open sandals, flip-flops, or any other type of shoe fails to cover the toes. Try not to wear loose-fitting shirts or pants. 4. Tie back long hair 5. No bags on the tops of the lab bench 6. Do not eat, drink, apply make-up, or remove contacts - No gum, candy, etc. Bioscience Safety BE CAREFUL AND USE COMMON SENSE!

9. Basic lab safety rules 7. No smoking in the building 8. Always be conscious of your hands – do not bite nails, touch eyes, etc. 9. Goggles and gloves are to be worn when instructed by me. - We will be working with chemicals that have potential to harm your eyes/skin and ruin your clothes (don’t wear something to lab that you don’t mind getting stained). 10. Waste disposal – Do not put harmful chemicals (e.g. organic solids/liquids) in the sinks. ASK IF YOU ARE UNSURE ABOUT WASTE DISPOSAL 11. Be careful when handling glass and other sharps - Never use cracked or broken glassware, never force glassware into a waste container, etc. 12. Wash your hands before leaving the lab – you may have been careful, but your neighbor may not be. Bioscience Safety BE CAREFUL AND USE COMMON SENSE!

10. Lab 1: Data Collection Key Lab equipment erlenmeyer flask Beaker Pipette scale Test tube Graduated cylinder

13. Lab 1: Data Collection Procedure 1.4: The effect of food type on yeast growth Purpose of the experiment: Add glucose to one test tube of yeast, add protein to another test tube of yeast and measure the amount of CO2 produced. As yeast grow, they release CO2. The amount of growth is proportional to and can be measured by the amount of CO2 given off. The independent variable in this experiment is ________ and the dependent variable is ______. a. Food molecule; water b. water; CO2 c. CO2; Food molecule d. Food molecule; CO2 The amount of CO2 produced is DEPENDENT on the amount of food molecule (independent variable) added.

14. Lab 1: Data Collection Procedure 1.4: The effect of food type on yeast growth Experiments need replications to verify naturally occurring variation of the results Each group will be conducting the same experiment; therefore there will 6 replications. Purpose of a control: To validate that the results are due only to the treatment (independent) variable. Each group will get three test tubes (instead of 12 as noted in the lab book). Test tube 1 will contain 5mLs of 5% glucose; test tube 2 will contain 5mLs of 5% protein and test tube three will contain 5mL of tap water . Follow procedure 1.4, steps 5 to 9

16. Lab 1: Data Collection Procedure 1.5: Quantify and summarize the data Analyze the data 1. Measure CO2 production in mm. 2. Calculate the mean CO2 production among the 6 groups. 3. Subtract the mean of CO2 production in the control from the mean of the CO2 production in the glucose test tube. Do the same for the Protein test tube. 4. Record the range of mms of both the Glucose test tube and Protein test tube. 5. Calculate the SD for Glucose and Protein Testing a Hypothesis Verify that the means are significantly different by calculating that means plus or minus ½ of the SD do not overlap. Fill out your calculations and answer the questions. Is your null hypothesis accepted or rejected?