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1. Process of testing bacterial inhibiting property ( 1 ) Put bacteria on all plates Spread plate Put all plates into incubator at 37 o C for 24 hours Put a piece of cotton on each plate
2. Process of testing bacterial inhibiting property ( 2 ) Move every piece of cotton from the plate in incubator to another plate with media Put all plates into incubator at 37 o C for 24 hours put all plates into incubator at 37 oC for 24 hours
3. Result of testing Staphylococcus aureus bacterial inhibiting property
11. Note : + Found less densely bacteria ++ Found medium densely bacteria +++ Found high densely bacteria ++ + S 1 X +++ ++ Biofile X ++ + Fluka X +++ +++ S 1 +++ ++ Biofile ++ + Fluka +++ +++ CONTROL Past to 48 hour Past to 24 hour Kind of Chitosan Staphylococcus aureus
20. Note : + Found less densely bacteria ++ Found medium densely bacteria +++ Found high densely bacteria +++ +++ S 1 X ++ ++ Biofile X ++ + Fluka X ++ ++ S 1 ++ + Biofile ++ + Fluka +++ +++ CONTROL Past to 48 hour Past to 24 hour Kind of Chitosan Escherichia coli
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23. 1. the bacteria in the cotton chitosan plate grow less densely than the bacteria in the cotton plate. It may be assumed that chitosan can decrease the growth of Staphylococcus aureus and Escherichia coli . C onclu sion