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Population Growth

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Stephen Taylor
Stephen Taylor

For my IB Biology Class - 5.3 Populations. To be used in conjunction with the Excel StatBook. (http://i-biology.net/ia/statexcel/). A series of hemacytometer slides to allow students to practice counting populations and plotting their growth.

EducationTechnologyHealth & Medicine
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Popula'on  Growth Stephen  Taylor
Why  are   What  do   you  think? there  more   ants  than   elephants? Clipart  people  from:  h8p://www.clker.com/search/krug/1
Why  are  there  more  ants   than  elephants? Explain  popula3on  growth  using  this  tutorial.  Present  your   work  as  a  group  poster.  Get  working!     Include:      -­‐  Reasons  for  the  shape  of  a  sigmoidal  curve    -­‐  Factors  which  limit  popula3on  growth    -­‐  Compara3ve  curves  for  ants  an  elephants hDp://goo.gl/VKWcR   Clipart  people  from:  h8p://www.clker.com/search/krug/1
How  does  glucose  concentra'on  affect  the  rate   of  growth  of  Saccharomyces  cerevisiae? h8p://faculty.baruch.cuny.edu/jwahlert/bio1003/fungi.html Yeast,  sugar,  water.   Reproduce  by  mitosis.   How  many  cells  are  in  my  flask? h8p://www.thesciencefair.com/ h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
How  does  glucose  concentra'on  affect  the  rate   of  growth  of  Saccharomyces  cerevisiae? Yeast,  sugar,  water.   Reproduce  by  mitosis.   How  many  cells  are  in  my  flask? Samples  can  be  counted     with  a  hemacytometer.       1mm  x  1mm  grid.     0.1mm  deep.       So  count  per  grid  (16  squares)   X  10,000  =  cells  ml-­‐1.   h8p://www.thesciencefair.com/ h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
How  does  glucose  concentra'on  affect  the  rate   of  growth  of  Saccharomyces  cerevisiae? Popula'on Yeast,  sugar,  water.   Reproduce  by  mitosis.   Sketch  some  predicted   popula'on  growth  curves  for   Time  (days) different  concentra'ons  of   glucose  solu'on.  Explain.   h8p://www.thesciencefair.com/ h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
“Controlling  the  independent  variable?” How  does  glucose  concentra'on     affect  the  rate  of  growth  of     0gl-­‐1 Water.     Saccharomyces  cerevisiae? 5g  glucose  dissolved  in  1l  water   5gl-­‐1 to  make  stock  solu'on.   10g  glucose  dissolved  in  1l  water   10gl-­‐1 to  make  stock  solu'on.   Ini'al  popula'on 15g  glucose  dissolved  in  1l  water   15gl-­‐1 to  make  stock  solu'on.   20g  glucose  dissolved  in  1l  water   20gl-­‐1 to  make  stock  solu'on.   h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
“Sufficient  relevant  data?” How  does  glucose  concentra'on     affect  the  rate  of  growth  of     0gl-­‐1 Saccharomyces  cerevisiae? Popn  1 Popn  2 Popn  3 Popn  4 Popn  5 5gl-­‐1 Popn  1 Popn  2 Popn  3 Popn  4 Popn  5 10gl-­‐1 Popn  1 Popn  2 Popn  3 Popn  4 Popn  5 Ini'al  popula'on 15gl-­‐1 Popn  1 Popn  2 Popn  3 Popn  4 Popn  5 20gl-­‐1 Popn  1 Popn  2 Popn  3 Popn  4 Popn  5 h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
“Sufficient  relevant  data?” How  does  glucose  concentra'on     affect  the  rate  of  growth  of     0gl-­‐1 Saccharomyces  cerevisiae? Agitate  and  take  random   Popn  1 Popn  2 Popn  3 Popn  4 Popn  5 samples  every  day.       5gl-­‐1 Cell  counts  using  a   Popn  1 Popn  2 hemacytometer.     Popn  5 Popn  3 Popn  4   10gl-­‐1 Calculate  popula'on  in   Popn  1 Popn  2 cells  3 l-­‐1.   n  4 Popn  5 Popn   m Pop Ini'al  popula'on 15gl-­‐1 To  be  even  more  reliable,   Popn  1 Popn  2 Popn  3e repeat   ach  sample  from    5 Popn  4 Popn each  popula'on  five  'mes! 20gl-­‐1 Popn  1 Popn  2 Popn  3 Popn  4 Popn  5 h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
“Controlled  variables?” How  does  glucose  concentra'on  affect  the  rate  of  growth  of  Saccharomyces  cerevisiae? Variables How  might  they  affect  the   How  exactly  could  they  be   results? controlled? h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
How  many  cells  are  in  my  flask  today? Associated  qualita1ve  data  (observa3ons):   •        •      •      •      Samples  can  be  counted     with  a  hemacytometer.       1mm  x  1mm  grid.     0.1mm  deep.       So  count  per  grid  (16  squares)   X  10,000  =  cells  ml-­‐1.   h8p://www.thesciencefair.com/ h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
Raw  and  Processed  Data Our  cell  counts  took  us  up  to  half  an  hour  per  sample.     To  get  ‘sufficient  relevant  data’,  we  would  need  to  count  5  x  5  samples  a  day.   To  be  really  reliable  we’d  need  to  do  each  of  those  five  'mes.       Even  IBDP  students  can’t  work  that  hard!       The  following  slides  are  a  simulated  set  of  data.     They  will  allow  us  to  make  use  of  a  full  set  of  data  for  DCP.     Get  comfortable…     Use  the  tools  in  the   Excel  StatBook  to   work  with  the  data.   h8p://i-­‐biology.net/ia/statexcel/ h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
Time  =  0  hours   (day  1)
0gl-­‐1  glucose Popula'on  1   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.   This  example  has  10   cells  in  16  squares.
0gl-­‐1  glucose Popula'on  2   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.   This  example  has  9   cells  in  16  squares.   One  cell  is  ‘out’.
0gl-­‐1  glucose Popula'on  3   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.   This  example  has  9   cells  in  16  squares.  
0gl-­‐1  glucose Popula'on  4   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  5   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  1   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  2   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  3   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  4   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  5   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  1   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  2   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  3   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  4   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  5   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  1   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  2   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  3   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  4   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  5   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  1   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  2   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  3   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  4   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  5   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
Time  =  24  hours   (day  2)
0gl-­‐1  glucose Popula'on  1   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  2   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  3   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  4   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  5   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  1   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  2   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  3   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  4   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  5   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  1   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  2   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  3   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  4   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  5   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  1   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  2   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  3   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  4   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  5   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  1   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  2   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  3   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  4   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  5   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
Time  =  48  hours   (day  3)
0gl-­‐1  glucose Popula'on  1   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  2   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  3   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  4   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  5   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  1   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  2   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  3   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  4   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  5   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  1   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  2   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  3   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  4   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  5   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  1   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  2   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  3   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  4   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  5   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  1   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  2   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  3   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  4   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  5   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
Time  =  72  hours   (day  4)
0gl-­‐1  glucose Popula'on  1   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  2   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  3   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  4   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  5   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  1   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  2   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  3   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  4   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  5   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  1   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  2   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  3   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  4   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  5   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  1   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  2   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  3   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  4   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  5   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  1   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  2   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  3   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  4   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  5   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
Time  =  96  hours   (day  5)
0gl-­‐1  glucose Popula'on  1   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  2   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  3   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  4   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
0gl-­‐1  glucose Popula'on  5   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  1   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  2   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  3   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  4   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
5gl-­‐1  glucose Popula'on  5   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  1   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  2   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  3   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  4   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
10gl-­‐1  glucose Popula'on  5   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  1   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  2   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  3   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  4   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
15gl-­‐1  glucose Popula'on  5   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  1   Time:    96  hours     Dilu'on:  1  in  2  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  2   Time:    96  hours     Dilu'on:  1  in  2  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  3   Time:    96  hours     Dilu'on:  1  in  2  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  4   Time:    96  hours     Dilu'on:  1  in  2  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
20gl-­‐1  glucose Popula'on  5   Time:    96  hours     Dilu'on:  1  in  2  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  

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Population Growth

  • 1. Popula'on  Growth Stephen  Taylor
  • 2. Why  are   What  do   you  think? there  more   ants  than   elephants? Clipart  people  from:  h8p://www.clker.com/search/krug/1
  • 3. Why  are  there  more  ants   than  elephants? Explain  popula3on  growth  using  this  tutorial.  Present  your   work  as  a  group  poster.  Get  working!     Include:      -­‐  Reasons  for  the  shape  of  a  sigmoidal  curve    -­‐  Factors  which  limit  popula3on  growth    -­‐  Compara3ve  curves  for  ants  an  elephants hDp://goo.gl/VKWcR   Clipart  people  from:  h8p://www.clker.com/search/krug/1
  • 4. How  does  glucose  concentra'on  affect  the  rate   of  growth  of  Saccharomyces  cerevisiae? h8p://faculty.baruch.cuny.edu/jwahlert/bio1003/fungi.html Yeast,  sugar,  water.   Reproduce  by  mitosis.   How  many  cells  are  in  my  flask? h8p://www.thesciencefair.com/ h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
  • 5. How  does  glucose  concentra'on  affect  the  rate   of  growth  of  Saccharomyces  cerevisiae? Yeast,  sugar,  water.   Reproduce  by  mitosis.   How  many  cells  are  in  my  flask? Samples  can  be  counted     with  a  hemacytometer.       1mm  x  1mm  grid.     0.1mm  deep.       So  count  per  grid  (16  squares)   X  10,000  =  cells  ml-­‐1.   h8p://www.thesciencefair.com/ h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
  • 6. How  does  glucose  concentra'on  affect  the  rate   of  growth  of  Saccharomyces  cerevisiae? Popula'on Yeast,  sugar,  water.   Reproduce  by  mitosis.   Sketch  some  predicted   popula'on  growth  curves  for   Time  (days) different  concentra'ons  of   glucose  solu'on.  Explain.   h8p://www.thesciencefair.com/ h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
  • 7. “Controlling  the  independent  variable?” How  does  glucose  concentra'on     affect  the  rate  of  growth  of     0gl-­‐1 Water.     Saccharomyces  cerevisiae? 5g  glucose  dissolved  in  1l  water   5gl-­‐1 to  make  stock  solu'on.   10g  glucose  dissolved  in  1l  water   10gl-­‐1 to  make  stock  solu'on.   Ini'al  popula'on 15g  glucose  dissolved  in  1l  water   15gl-­‐1 to  make  stock  solu'on.   20g  glucose  dissolved  in  1l  water   20gl-­‐1 to  make  stock  solu'on.   h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
  • 8. “Sufficient  relevant  data?” How  does  glucose  concentra'on     affect  the  rate  of  growth  of     0gl-­‐1 Saccharomyces  cerevisiae? Popn  1 Popn  2 Popn  3 Popn  4 Popn  5 5gl-­‐1 Popn  1 Popn  2 Popn  3 Popn  4 Popn  5 10gl-­‐1 Popn  1 Popn  2 Popn  3 Popn  4 Popn  5 Ini'al  popula'on 15gl-­‐1 Popn  1 Popn  2 Popn  3 Popn  4 Popn  5 20gl-­‐1 Popn  1 Popn  2 Popn  3 Popn  4 Popn  5 h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
  • 9. “Sufficient  relevant  data?” How  does  glucose  concentra'on     affect  the  rate  of  growth  of     0gl-­‐1 Saccharomyces  cerevisiae? Agitate  and  take  random   Popn  1 Popn  2 Popn  3 Popn  4 Popn  5 samples  every  day.       5gl-­‐1 Cell  counts  using  a   Popn  1 Popn  2 hemacytometer.     Popn  5 Popn  3 Popn  4   10gl-­‐1 Calculate  popula'on  in   Popn  1 Popn  2 cells  3 l-­‐1.   n  4 Popn  5 Popn   m Pop Ini'al  popula'on 15gl-­‐1 To  be  even  more  reliable,   Popn  1 Popn  2 Popn  3e repeat   ach  sample  from    5 Popn  4 Popn each  popula'on  five  'mes! 20gl-­‐1 Popn  1 Popn  2 Popn  3 Popn  4 Popn  5 h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
  • 10. “Controlled  variables?” How  does  glucose  concentra'on  affect  the  rate  of  growth  of  Saccharomyces  cerevisiae? Variables How  might  they  affect  the   How  exactly  could  they  be   results? controlled? h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
  • 11. How  many  cells  are  in  my  flask  today? Associated  qualita1ve  data  (observa3ons):   •        •      •      •      Samples  can  be  counted     with  a  hemacytometer.       1mm  x  1mm  grid.     0.1mm  deep.       So  count  per  grid  (16  squares)   X  10,000  =  cells  ml-­‐1.   h8p://www.thesciencefair.com/ h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
  • 12. Raw  and  Processed  Data Our  cell  counts  took  us  up  to  half  an  hour  per  sample.     To  get  ‘sufficient  relevant  data’,  we  would  need  to  count  5  x  5  samples  a  day.   To  be  really  reliable  we’d  need  to  do  each  of  those  five  'mes.       Even  IBDP  students  can’t  work  that  hard!       The  following  slides  are  a  simulated  set  of  data.     They  will  allow  us  to  make  use  of  a  full  set  of  data  for  DCP.     Get  comfortable…     Use  the  tools  in  the   Excel  StatBook  to   work  with  the  data.   h8p://i-­‐biology.net/ia/statexcel/ h8p://www.clker.com/clipart-­‐empty-­‐erlenmeyer-­‐flask.html
  • 13. Time  =  0  hours   (day  1)
  • 14. 0gl-­‐1  glucose Popula'on  1   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.   This  example  has  10   cells  in  16  squares.
  • 15. 0gl-­‐1  glucose Popula'on  2   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.   This  example  has  9   cells  in  16  squares.   One  cell  is  ‘out’.
  • 16. 0gl-­‐1  glucose Popula'on  3   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.   This  example  has  9   cells  in  16  squares.  
  • 17. 0gl-­‐1  glucose Popula'on  4   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 18. 0gl-­‐1  glucose Popula'on  5   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 19. 5gl-­‐1  glucose Popula'on  1   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 20. 5gl-­‐1  glucose Popula'on  2   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 21. 5gl-­‐1  glucose Popula'on  3   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 22. 5gl-­‐1  glucose Popula'on  4   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 23. 5gl-­‐1  glucose Popula'on  5   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 24. 10gl-­‐1  glucose Popula'on  1   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 25. 10gl-­‐1  glucose Popula'on  2   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 26. 10gl-­‐1  glucose Popula'on  3   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 27. 10gl-­‐1  glucose Popula'on  4   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 28. 10gl-­‐1  glucose Popula'on  5   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 29. 15gl-­‐1  glucose Popula'on  1   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 30. 15gl-­‐1  glucose Popula'on  2   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 31. 15gl-­‐1  glucose Popula'on  3   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 32. 15gl-­‐1  glucose Popula'on  4   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 33. 15gl-­‐1  glucose Popula'on  5   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 34. 20gl-­‐1  glucose Popula'on  1   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 35. 20gl-­‐1  glucose Popula'on  2   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 36. 20gl-­‐1  glucose Popula'on  3   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 37. 20gl-­‐1  glucose Popula'on  4   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 38. 20gl-­‐1  glucose Popula'on  5   Time:    0  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 39. Time  =  24  hours   (day  2)
  • 40. 0gl-­‐1  glucose Popula'on  1   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 41. 0gl-­‐1  glucose Popula'on  2   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 42. 0gl-­‐1  glucose Popula'on  3   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 43. 0gl-­‐1  glucose Popula'on  4   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 44. 0gl-­‐1  glucose Popula'on  5   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 45. 5gl-­‐1  glucose Popula'on  1   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 46. 5gl-­‐1  glucose Popula'on  2   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 47. 5gl-­‐1  glucose Popula'on  3   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 48. 5gl-­‐1  glucose Popula'on  4   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 49. 5gl-­‐1  glucose Popula'on  5   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 50. 10gl-­‐1  glucose Popula'on  1   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 51. 10gl-­‐1  glucose Popula'on  2   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 52. 10gl-­‐1  glucose Popula'on  3   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 53. 10gl-­‐1  glucose Popula'on  4   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 54. 10gl-­‐1  glucose Popula'on  5   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 55. 15gl-­‐1  glucose Popula'on  1   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 56. 15gl-­‐1  glucose Popula'on  2   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 57. 15gl-­‐1  glucose Popula'on  3   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 58. 15gl-­‐1  glucose Popula'on  4   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 59. 15gl-­‐1  glucose Popula'on  5   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 60. 20gl-­‐1  glucose Popula'on  1   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 61. 20gl-­‐1  glucose Popula'on  2   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 62. 20gl-­‐1  glucose Popula'on  3   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 63. 20gl-­‐1  glucose Popula'on  4   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 64. 20gl-­‐1  glucose Popula'on  5   Time:    24  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 65. Time  =  48  hours   (day  3)
  • 66. 0gl-­‐1  glucose Popula'on  1   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 67. 0gl-­‐1  glucose Popula'on  2   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 68. 0gl-­‐1  glucose Popula'on  3   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 69. 0gl-­‐1  glucose Popula'on  4   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 70. 0gl-­‐1  glucose Popula'on  5   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 71. 5gl-­‐1  glucose Popula'on  1   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 72. 5gl-­‐1  glucose Popula'on  2   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 73. 5gl-­‐1  glucose Popula'on  3   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 74. 5gl-­‐1  glucose Popula'on  4   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 75. 5gl-­‐1  glucose Popula'on  5   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 76. 10gl-­‐1  glucose Popula'on  1   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 77. 10gl-­‐1  glucose Popula'on  2   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 78. 10gl-­‐1  glucose Popula'on  3   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 79. 10gl-­‐1  glucose Popula'on  4   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 80. 10gl-­‐1  glucose Popula'on  5   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 81. 15gl-­‐1  glucose Popula'on  1   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 82. 15gl-­‐1  glucose Popula'on  2   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 83. 15gl-­‐1  glucose Popula'on  3   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 84. 15gl-­‐1  glucose Popula'on  4   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 85. 15gl-­‐1  glucose Popula'on  5   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 86. 20gl-­‐1  glucose Popula'on  1   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 87. 20gl-­‐1  glucose Popula'on  2   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 88. 20gl-­‐1  glucose Popula'on  3   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 89. 20gl-­‐1  glucose Popula'on  4   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 90. 20gl-­‐1  glucose Popula'on  5   Time:    48  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 91. Time  =  72  hours   (day  4)
  • 92. 0gl-­‐1  glucose Popula'on  1   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 93. 0gl-­‐1  glucose Popula'on  2   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 94. 0gl-­‐1  glucose Popula'on  3   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 95. 0gl-­‐1  glucose Popula'on  4   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 96. 0gl-­‐1  glucose Popula'on  5   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 97. 5gl-­‐1  glucose Popula'on  1   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 98. 5gl-­‐1  glucose Popula'on  2   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 99. 5gl-­‐1  glucose Popula'on  3   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 100. 5gl-­‐1  glucose Popula'on  4   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 101. 5gl-­‐1  glucose Popula'on  5   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 102. 10gl-­‐1  glucose Popula'on  1   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 103. 10gl-­‐1  glucose Popula'on  2   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 104. 10gl-­‐1  glucose Popula'on  3   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 105. 10gl-­‐1  glucose Popula'on  4   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 106. 10gl-­‐1  glucose Popula'on  5   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 107. 15gl-­‐1  glucose Popula'on  1   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 108. 15gl-­‐1  glucose Popula'on  2   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 109. 15gl-­‐1  glucose Popula'on  3   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 110. 15gl-­‐1  glucose Popula'on  4   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 111. 15gl-­‐1  glucose Popula'on  5   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 112. 20gl-­‐1  glucose Popula'on  1   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 113. 20gl-­‐1  glucose Popula'on  2   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 114. 20gl-­‐1  glucose Popula'on  3   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 115. 20gl-­‐1  glucose Popula'on  4   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 116. 20gl-­‐1  glucose Popula'on  5   Time:    72  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 117. Time  =  96  hours   (day  5)
  • 118. 0gl-­‐1  glucose Popula'on  1   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 119. 0gl-­‐1  glucose Popula'on  2   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 120. 0gl-­‐1  glucose Popula'on  3   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 121. 0gl-­‐1  glucose Popula'on  4   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 122. 0gl-­‐1  glucose Popula'on  5   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 123. 5gl-­‐1  glucose Popula'on  1   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 124. 5gl-­‐1  glucose Popula'on  2   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 125. 5gl-­‐1  glucose Popula'on  3   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 126. 5gl-­‐1  glucose Popula'on  4   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 127. 5gl-­‐1  glucose Popula'on  5   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 128. 10gl-­‐1  glucose Popula'on  1   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 129. 10gl-­‐1  glucose Popula'on  2   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 130. 10gl-­‐1  glucose Popula'on  3   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 131. 10gl-­‐1  glucose Popula'on  4   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 132. 10gl-­‐1  glucose Popula'on  5   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 133. 15gl-­‐1  glucose Popula'on  1   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 134. 15gl-­‐1  glucose Popula'on  2   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 135. 15gl-­‐1  glucose Popula'on  3   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 136. 15gl-­‐1  glucose Popula'on  4   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 137. 15gl-­‐1  glucose Popula'on  5   Time:    96  hours     Dilu'on:  none  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 138. 20gl-­‐1  glucose Popula'on  1   Time:    96  hours     Dilu'on:  1  in  2  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 139. 20gl-­‐1  glucose Popula'on  2   Time:    96  hours     Dilu'on:  1  in  2  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 140. 20gl-­‐1  glucose Popula'on  3   Time:    96  hours     Dilu'on:  1  in  2  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 141. 20gl-­‐1  glucose Popula'on  4   Time:    96  hours     Dilu'on:  1  in  2  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 142. 20gl-­‐1  glucose Popula'on  5   Time:    96  hours     Dilu'on:  1  in  2  Count  to  16  big  squares   or  100  cells  first,   whichever  is  first.  
  • 144. A  crea've  commons  work  by  Stephen  Taylor.  Do  not  re-­‐host  on  other  sites.  Embedding  is  fine.