Protein Synthesis (transcription & translation) running dication class activity for IB Biology.

1. Protein synthesis running dictation
1. Complete the DNA by adding the antisense (complementary) strand.
Sense
SET A
T G A T G C C C T T T C A C T G T C G G G T A G T C C C T G A T T A C A G G T G A T
Antisense
(COMPLEMENTARY)
-------------------cut----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2. Transcription (you are RNA polymerase)
SET A
Transcribe the antisense strand by complementary base-pairing it with RNA nucleotides.
mRNA
Remember: in RNA Thymine (T) is NOT used. It is replaced with Uracil (U).
What does the ‘m’ in mRNA mean?
How does the mRNA strand compare to the original DNA Sense strand?
Send to the ribosome, through the nuclear membrane.
-------------------cut-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
3. Identify the codons and write the amino acid sequence. You are the ribosome. (A)
 Hint: scan through to find the START codon first (methionine).
Sequence of amino acids:
How many codons?
How many amino acids?
How many base pairs?
Stephen Taylor
i-Biology.net
Resources: http://i-biology.net/ibdpbio/01-cells-and-energy/transcription-and-translation-inc-ahl-73-74/

2. Protein synthesis running dictation
1. Complete the DNA by adding the antisense (complementary) strand.
Sense
SET B
A T T A T G T T C C G A G A T T A C C A G A T T A C C A C G G A G T C A C C G T A A
Antisense
(COMPLEMENTARY)
-------------------cut----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2. Transcription (you are RNA polymerase)
SET B
mRNA
Remember: in RNA Thymine (T) is NOT used. It is replaced with Uracil (U).
What does the ‘m’ in mRNA mean?
Send to the ribosome, through the nuclear membrane.
----------------------cut----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
3. Identify the codons and write the amino acid sequence. You are the ribosome. (B)
 Hint: scan through to find the START codon first (methionine).
Sequence:
1. How many codons?
2. How many amino acids?
3. How many base pairs?
Stephen Taylor
i-Biology.net
Resources: http://i-biology.net/ibdpbio/01-cells-and-energy/transcription-and-translation-inc-ahl-73-74/

3. Protein synthesis running dictation
1. Complete the DNA.
Sense
Antisense
(COMPLEMENTARY)
SET A Answers
T G A T G C C C T T T C A C T G T C G G G T A G T C C C T G A T T A C A G G T G A T
a c t a c g g g a a a g t g a c a g c c c a t c a g g g a c t a a t g t c c a c t a
-------------------------cut ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2. Transcription (you are RNA polymerase)
mRNA
SET A Answers
U G A U G C C C U U U C A C U G U C G G G U A G U C C C U G A U U A C A G G U G A U
Find start codon:
MET
pro
phe
his
cys
arg
val
val
pro
asp
tyr
arg
STOP
Send to the ribosome, through the nuclear membrane.
---------------------------cut -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
3. Identify the codons and write the amino acid sequence. You are the ribosome.
Sequence:
See above.
How many codons?13
How many amino acids?12
How many base pairs?39 (in the coding section of the gene)
Stephen Taylor
i-Biology.net
Resources: http://i-biology.net/ibdpbio/01-cells-and-energy/transcription-and-translation-inc-ahl-73-74/
SET A Answers

4. Protein synthesis running dictation
1. Complete the DNA.
Sense
Antisense
(COMPLEMENTARY)
SET B Answers
A T T A T G T T C C G A G A T T A C C A G A T T A C C A C G G A G T C A C C G T A A
t a a t a c a a g g a t c t a a t g g t c t a a t g g t g c c t c a g t g g c a t t
-------------------------------cut ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2. Transcription (you are RNA polymerase)
mRNA
SET B Answers
a u u A U G U U C C G A G A U U A C C A G A U U A C C A C G G A G U C A C C G U A A
Find start codon
MET
phe
arg
asp
tyr.
gln
lie
lhr
lhr
glu
ser
pro
STOP
Send to the ribosome, through the nuclear membrane.
---------------------------------cut ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
3. Identify the codons and write the amino acid sequence. You are the ribosome.
Sequence:
As above.
How many codons? 13
How many amino acids? 12
How many base pairs? 39 (in the coding section of the gene)
Stephen Taylor
i-Biology.net
Resources: http://i-biology.net/ibdpbio/01-cells-and-energy/transcription-and-translation-inc-ahl-73-74/
SET B Answers