FMDV

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Santé & Médecine Technologie

Promising Multiple-Epitope Recombinant Vaccine against Foot-and-Mouth Disease Virus Type O in Swine Jung-Jun Shao, et al. Hong Kong University Of Science and Technology Clinical and Vaccine Immunology: January 2011

Background Foot-and-Mouth Disease Virus (FMDV) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Background Foot-and-Mouth Disease Virus (FMDV) Continues ,[object Object],[object Object],[object Object]

Current Treatments ,[object Object],[object Object],[object Object],[object Object]

Modified FMDV Type O Vaccine ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Methods ,[object Object],[object Object],[object Object],[object Object]

Methods ,[object Object],[object Object],[object Object],[object Object],[object Object]

Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Materials & Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Results Expression and characterization of recombinant RE-scIgG protein Recombinant expression plasmid pET-22b-RE-scIgG constructed successfully Specific band of 52 kDa could be seen by 12% SDS-PAGE; no band found in lysates of E.coli /pET-22b cells In the Ni affinity column (Lane 5), SDS-PAGE showed recombinant protein purity of 95% FIG. 3: Lane 1: protein molecular size markers, Lane 2: plasmid pET-22b induced with IPTG after 4 hours, Lane 3: recombinant plasmid pET-22b-RE-scIgG induced with IPTG after 4 hours FIG. 4: Lane 1: protein molecular size markers, Lane 2: recombinant plasmid pET-22b-RE-scIgG preinduced with IPTG, Lane 3: recombinant plasmid pET-22b-RE-scIgG induced with IPTG after 6 hours, Lane 4: deposition of recombinant protein RE-scIgG after sonification, Lane 5: protein RE-scIgG purified with Ni-NTA agarose resin

Results ,[object Object],[object Object],[object Object],[object Object],[object Object],FIG 5: Lane 1: protein molecular size markers, Lane 2: RE-scIgG reacted with serum negative for FMDV, Lane 3: RE-scIgG reacted with positive serum from cattle infected with FMDV FMDV (Type O) antibodies recognized RE-scIgG

Results ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Results TABLE 2: Titers of antibodies against FMDV and swine challenge results

Results ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Results ,[object Object],[object Object],[object Object],[object Object]

Results ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Discussion ,[object Object],[object Object],[object Object],[object Object],[object Object]

Discussion ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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