http://persatuangenetikmalaysia.com/index_files/Page19319.html

1. HANIF BIN ABDUL BAHAR
INSTITUTE OF TROPICAL AGRICULTURE
UNIVERSITI PUTRA MALAYSIA
Supervisory committee

2. A VITAMIN E GENE FROM OIL PALM:
ISOLATION OF
GAMMA-TOCOPHEROL METHYLTRANSFERASE
FULL GENE STRUCTURE

3. INTRODUCTION &
LITERATURE REVIEW

4. Introduction
Oil Palm Cultivation
• Oil Palm is a tropical perennial monocot species in the
order Arecales of the family Aracaceae.
• The African Oil Palm, Elaeis guineensis is native to
West Africa, occurring between Angola and Gambia,
while the American Oil Palm Elaeis oleifera is native to
tropical Central America and South America.
(Dransfield et al., 2005)
• Oil palm was first brought to Malaya in early twentieth
century as ornamental plant. It is now one of the main
commodity crop in Malaysia.

5. Vitamin E in Oil Palm
• Vitamin E consists of tocopherols and
tocotrienols .
• Vitamin E in palm oil is made up of 70%
tocotrienol and 30% of tocopherol.
• Palm oil is nature’s richest source of tocotrienols.
• Whereas most other vegetable oils consists of
only tocopherols.
(Koh, 2003)

6. Main Objectives
To isolate full length cDNA of gama-
tocopherol methyltransferase (γ-TMT)
gene from oil palm.
To obtain γ-TMT gene full gene structure
DNA sequence.
To identify single nucleotide polymorphism
(SNP) in γ-TMT gene.

7. Literature Review
Vitamin E Chemical Structure
Tocopherol Tocotrienol
• Tocopherols and tocotrienols consists of a chromanol head
group with 1,2 or 3 methyl groups and an isoprenoid side
chain. (Chen et al., 2006).
• Tocopherols have a saturated phytyl tail, while tocotrienols
have a 3-fold unsaturated side chain. (DellaPenna & Pagson,2005).
• Tocopherols and tocotrienols differ only in the number and
position of methyl substituents on the aromatic ring with α-
having three, β- and γ- having two, and δ- tocopherol having
only one. (Hunter & Cahoon, 2007).

8. Vitamin E biosynthesis pathway:

9. Health Benefits of Vitamin E
Inhibit growth of
Reduce blood cancer cells
cholesterol
Health
benefits
antioxidant/ neuroprotection
antiaging
inhibit
angiogenesis

10. METHODOLOGY

11. Overview Methodology
A. Full length cDNA Gene Isolation
Oil Palm RNA RACE technique
mesocarp extraction
Full length cDNA gene

12. B. Full length Gene Structure
Oil DNA PCR GenomeWalker
Palm extraction
leaves
Full length gene
sturcture

13. RESULTS &
DISCUSSIONS

14. For more information, please contact me via email:
hanif_biotech@yahoo.com
Or Facebook:
fb.me/hanif85

15. Acknowledgement