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Ian Pringle
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Ian A. Pringle GeneMedicine Group University of Oxford & The UK Cystic Fibrosis Gene Therapy Consortium http://www.cfgenetherapy.org.uk Zero CpG plasmids eliminate the inflammatory response associated with lung non-viral gene transfer
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[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Alton et al ., 1999. Lancet, 353, 947-953. Cationic lipid-mediated CFTR gene transfer to the lungs and nose of patients with Cystic Fibrosis: a double-bling placebo controlled trial.
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],Backbone - Similar To That Used In Multiple Phase I Trials In Mid 1990’s
[object Object],Backbone - Similar To That Used In Multiple Phase I Trials In Mid 1990’s = 1 CpG Motif
[object Object],Backbone - Reduced CpG Content Overall 40 % fewer CpGs Backbone - Similar To That Used In Multiple Phase I Trials In Mid 1990’s
[object Object],Backbone - Similar To That Used In Multiple Phase I Trials In Mid 1990’s Backbone - Reduced CpG Content Overall 40 % fewer CpGs
[object Object],BALB/c n=10 GL67 Instillation Cells IFN-  IL-12 TNF- 
[object Object],BALB/c n=10 GL67 Instillation Cells IFN-  IL-12 TNF- 
[object Object],BALB/c n=10 GL67 Instillation Cells IFN-  IL-12 TNF- 
[object Object],[object Object],BALB/c n=10 GL67 Instillation Mann Whitney P>0.05 Cells IFN-  IL-12 TNF- 
[object Object],[object Object],[object Object],[object Object]
[object Object],BALB/c n=10 GL67 Instillation Cells IFN-  IL-12 TNF- 
[object Object],BALB/c n=10 GL67 Instillation Cells IFN-  IL-12 TNF- 
[object Object],BALB/c n=10 GL67 Instillation Cells IFN-  IL-12 TNF-  ,[object Object],Mann Whitney P>0.05
[object Object],[object Object],[object Object],[object Object]
[object Object]
[object Object],BALB/c n=10 GL67 Instillation Cells IFN-  IL-12 TNF- 
[object Object],BALB/c n=10 GL67 Instillation Cells IFN-  IL-12 TNF- 
[object Object],BALB/c n=10 GL67 Instillation Cells IFN-  IL-12 TNF-  ,[object Object],Mann Whitney P<0.05
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],US FDA Docket No 96N-0400. Points to consider on Plasmid DNA Vaccines for Preventative Infectious Disease Indications
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],BALB/c n=6 GL67 aerosol
[object Object],BALB/c n=6 GL67 aerosol
[object Object],BALB/c n=6 GL67 aerosol
[object Object],BALB/c n=6 GL67 aerosol
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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  • 1. Ian A. Pringle GeneMedicine Group University of Oxford & The UK Cystic Fibrosis Gene Therapy Consortium http://www.cfgenetherapy.org.uk Zero CpG plasmids eliminate the inflammatory response associated with lung non-viral gene transfer
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