Obsessed with Noni Research

1. David Wadsworth Vice President Global Product Strategy

2. A Passion for Noni Research

5. 7 bottles/day/7 years 2155 days 17,885 bottles 4,471 cases 7 Liters of TNJ/Day

6. $300,000. 2 Years.

9. Obsessed with Noni Research

12. Placebo is TNJ without Noni

13. TNJ is a Superior Antioxidant

14. TNJ Aids in Quality of Life

15. TNJ Increases Energy & Physical Performance

20. 2001-2006 Journal Publications

21. 2007 Journal Publications

22. 2008 Journal Publications—so far

24. TNI and Its Research on Noni Johannes Westendorf University of Hamburg Germany

25. Soon after its foundation, Morinda Inc. began with systematic research on noni. They built up their own Department of Research and Development (R&D) and a Health and Technical Advisory Board (HTAB) , later replaced by the Global Advisory Board (GAB).

26. Foundation of the company Morinda Inc. Ralph Heinicke publication on noni Furusawa and Hirazumi Anti-cancer effects of noni

27. Topic of research No. of publications % of total ______________________________________________________ Ethnopharmacology 84 19.5 Chemistry 74 17.2 Toxicology 42 9.76 Cancer&Immunsystem 37 8.60 Pharmacology 33 7.67 Inflammation 25 5.81 Antimicrobial 24 5.58 Antioxidant 24 5.58 Review articles 24 5.58 Botany of Noni 18 4.18 Other topics 17 3.95 Noni Plant cell culture 16 3.72 Hormonal action of Noni 7 1.62 Product quality 5 1.16 Total 430 100

28. Ethno-Pharmacology

29. The old Polynesians took noni juice to increase their endurance at long fishing trips. Do we have evidence that noni juice increases the endurance?

30. Experiment on athletes (N=20)

31. Cortisol plasma level Day time Cortisol blood level (ng/ml) Day light Thalamus Hypothalamus Pituitary Adrenals Cortisol ACTH CRF Start End

32. 200 ml TNJ

34. Jonas Student Susanne Student Johannes Supervisor

35. Simla Postdoc

36. Cortisol blood level (mean of N=4) Values are Mean of 4

37. The endurance is very much dependent on the body's load with antioxidants. Endogenous antioxidants Katalase Superoxid dismutase Glutathion NADH/NADPH Exogenous antioxidants Vitamin C Vitamin E Flavonoids Catechines Selenium et c.

38. Exogenous antioxidants have to be absorbed and distributed to the site, where the radicals are, in order to be effective Endogenous antioxidants are always present in our body. Their concentration can be influenced by exogenous compounds (food ingredients)

39. Role of Antioxidants O 2 H 2 O + CO 2 SAR Hydroxyl- radicals Tissue damage Antioxidants destroy Glucose Inflammation Ageing Cancer

40. Depletion of antioxidants causes exhaustion (fatigue)

41. Measurement of Antioxidant capacity The measurement of the antioxidant capacity (AOA) should be performed inside of our body in order to be predictive

42. Antioxidative potency of blood, one hour after intake of 200ml of test compound 20 volunteers per group Serum Red blood cells O.118 0.055 0.6 2.7

45. Animals on the Marquesa islands eat noni leaf and fruit. They are healthy, and especially their liver is very clean.

46. As a result of TNI’s intense studies on the safety of TNJ, the EU commission stated: “ Noni juice is safe for human consumption”

47. This statement was confirmed by the EU after some publications stated that noni juice could damage the liver.

48. Research on the quality of TNJ TNI invested enormous money and effort in the definition and maintenance of the quality of their noni products. The company controls and defines every step from the harvest until distribution of the products. Other companies often buy noni concentrates prepared at tropical islands far from their control, dilute it and add preservatives and artificial flavoring compounds.

49. CPX = (2 E ,4 Z ,7 Z )-Decatrienoic acid) Other polyunsaturated fatty acids (PUFA) Quercetin Scopoletin HPLC-chromatogram of a TNJ ethylacetat extract with marker compounds indicated

50. HPLC-Analysis of a competitor noni (black) and TNJ (red) Collection of peak and further analysis bc GC/MS

51. GC/MS analysis of peak isolated from HPLC run of the competitor noni

56. Two Additional Novel Food Applications

57. Extension of Use of Fruit

58. Noni Leaf as Food

59. Benefits of Noni Leaf

60. Green tea infusion Noni leaf infusion

61. World Class Research