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Chapter Five Protein Purification and  Characterization Techniques
Isolation of Proteins from Cells ,[object Object],[object Object],[object Object]
How We Get Proteins Out of Cells
Salting Out ,[object Object],[object Object],[object Object],[object Object],[object Object]
Differential Centrifugation ,[object Object],[object Object]
Column Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Column Chromatography
Size-Exclusion/Gel-Filtration ,[object Object],[object Object],[object Object],[object Object]
Size Exclusion/Gel-filtration (Cont’d)
Affinity Chromatography ,[object Object],[object Object]
Ion Exchange ,[object Object],[object Object],[object Object]
Electrophoresis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Electrophoresis (Cont’d) ,[object Object],[object Object],[object Object]
Isoelectric Focusing ,[object Object],[object Object],[object Object],[object Object]
Primary Structure Determination ,[object Object],[object Object],[object Object],[object Object],[object Object]
Primary Structure Determination
Protein Cleavage ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Peptide Digestion
Cleavage by CnBr ,[object Object]
Determining Protein Sequence ,[object Object],[object Object],[object Object]
Peptide Sequencing ,[object Object],[object Object],[object Object]
Peptide Sequencing

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Campbell6e lecture ch5