SlideShare une entreprise Scribd logo
1  sur  15
Télécharger pour lire hors ligne
from strawberries
 1 resealable plastic bag
 Strawberries (fresh or frozen)
 2 teaspoons of dish detergent
 1 teaspoon of salt
 ½ cup of deionized water
 2 plastic cups
 Filtering apparatus
 Ice cold 90 percent rubbing alcohol
 1 wooden popsicle stick or plastic coffee
stirrer
 Gloves
 1 vial
 Pull off any green leaves on the strawberries.
 Put some strawberries into the plastic bag,
remove the air and seal it.
 Gently smash the strawberries and crush
them completely. This starts to break open
the cells and release the DNA.
It's all about Science!
 In a plastic cup make your buffer solution:
mix together the deionized water, the salt
and the detergent.
 Add 2 teaspoons of the buffer solution into
the bag with the strawberries. This will
further break open the cells.
 Reseal the bag and continue smashing the
strawberries gently for a while.
It's all about Science!
 Place the coffee filter inside the other
plastic cup.
 Open the bag and pour the strawberry liquid
into the filter. You can gently squeeze the
remaining liquid into the cup.
 Put approximately 4 ml of the liquid into the
vial.
It's all about Science!
 Tilt the vial a little bit and then pour slow
and carefully about 16 ml of cold rubbing
alcohol (until it reaches 20 ml). Do NOT mix
or stir. You have just isolated the DNA from
the rest of the material contained in the
cells of the strawberry.
 Within a few seconds, watch for the
development of a white cloudy substance
(DNA) in the top layer above the strawberry
extract layer.
 Pick up the DNA using a plastic coffee stirrer
or a wooden stick.
It's all about Science!
This is your DNA…
It's all about Science!
You can also save the DNA you
extracted into a small vial
containing alcohol. In the vial with
the strawberry liquid and the
alcohol, DNA will continue appearing
and if you shake it a little bit, it will
have a smooth reddish color.
It's all about Science!
You can keep both vials in normal
atmosphere conditions without
damaging the DNA. Just have in
mind to change the alcohol after
a few years!

Contenu connexe

Similaire à It's all about Science!

DrinksSum16_Zen and Tonic
DrinksSum16_Zen and TonicDrinksSum16_Zen and Tonic
DrinksSum16_Zen and TonicBrittany Falk
 
2 Dna Extraction Lab
2  Dna Extraction Lab2  Dna Extraction Lab
2 Dna Extraction LabBob Smullen
 
Easy fruit & herb flavored waters
Easy fruit & herb flavored watersEasy fruit & herb flavored waters
Easy fruit & herb flavored watersAndrew Forster
 
Food Preservation Strawberries
Food Preservation   StrawberriesFood Preservation   Strawberries
Food Preservation StrawberriesHome Makers
 
Secret document
Secret documentSecret document
Secret documentssagal
 
Ginger beer
Ginger beerGinger beer
Ginger beereflcafe
 
C12 Dna Extraction
C12 Dna ExtractionC12 Dna Extraction
C12 Dna Extractionswalden01
 
Dna Extraction From Wheat Germ
Dna Extraction From Wheat GermDna Extraction From Wheat Germ
Dna Extraction From Wheat GermTeriT
 
WORLD CLASS Canada Bartender of the Year Cocktail Booklet - Grant Sceney OP ENG
WORLD CLASS Canada Bartender of the Year Cocktail Booklet - Grant Sceney OP ENGWORLD CLASS Canada Bartender of the Year Cocktail Booklet - Grant Sceney OP ENG
WORLD CLASS Canada Bartender of the Year Cocktail Booklet - Grant Sceney OP ENGKris Steed
 
Lesson 1 - Cleaning and Maintenance.pptx
Lesson 1 - Cleaning and Maintenance.pptxLesson 1 - Cleaning and Maintenance.pptx
Lesson 1 - Cleaning and Maintenance.pptxTeacherDennis1
 
Cleaning products presentation
Cleaning products presentationCleaning products presentation
Cleaning products presentationalijsheridan
 

Similaire à It's all about Science! (20)

DrinksSum16_Zen and Tonic
DrinksSum16_Zen and TonicDrinksSum16_Zen and Tonic
DrinksSum16_Zen and Tonic
 
Cleaners Class Slides
Cleaners Class SlidesCleaners Class Slides
Cleaners Class Slides
 
Bar notes
Bar notesBar notes
Bar notes
 
Clean Home Guide
Clean Home GuideClean Home Guide
Clean Home Guide
 
2 Dna Extraction Lab
2  Dna Extraction Lab2  Dna Extraction Lab
2 Dna Extraction Lab
 
Easy fruit & herb flavored waters
Easy fruit & herb flavored watersEasy fruit & herb flavored waters
Easy fruit & herb flavored waters
 
00 Water- Emergency Drinking Water- mirror
00  Water- Emergency Drinking Water- mirror00  Water- Emergency Drinking Water- mirror
00 Water- Emergency Drinking Water- mirror
 
Food Preservation Strawberries
Food Preservation   StrawberriesFood Preservation   Strawberries
Food Preservation Strawberries
 
Ecological perfumes
Ecological perfumesEcological perfumes
Ecological perfumes
 
Secret document
Secret documentSecret document
Secret document
 
Ginger beer
Ginger beerGinger beer
Ginger beer
 
Xmas party
Xmas partyXmas party
Xmas party
 
Bar management
Bar managementBar management
Bar management
 
C12 Dna Extraction
C12 Dna ExtractionC12 Dna Extraction
C12 Dna Extraction
 
Dna Extraction From Wheat Germ
Dna Extraction From Wheat GermDna Extraction From Wheat Germ
Dna Extraction From Wheat Germ
 
WORLD CLASS Canada Bartender of the Year Cocktail Booklet - Grant Sceney OP ENG
WORLD CLASS Canada Bartender of the Year Cocktail Booklet - Grant Sceney OP ENGWORLD CLASS Canada Bartender of the Year Cocktail Booklet - Grant Sceney OP ENG
WORLD CLASS Canada Bartender of the Year Cocktail Booklet - Grant Sceney OP ENG
 
Lesson 1 - Cleaning and Maintenance.pptx
Lesson 1 - Cleaning and Maintenance.pptxLesson 1 - Cleaning and Maintenance.pptx
Lesson 1 - Cleaning and Maintenance.pptx
 
Cleaning products presentation
Cleaning products presentationCleaning products presentation
Cleaning products presentation
 
Dna from a banana lab report
Dna from a banana lab report Dna from a banana lab report
Dna from a banana lab report
 
Dna from a banana lab report
Dna from a banana lab report Dna from a banana lab report
Dna from a banana lab report
 

Dernier

Exploration Method’s in Archaeological Studies & Research
Exploration Method’s in Archaeological Studies & ResearchExploration Method’s in Archaeological Studies & Research
Exploration Method’s in Archaeological Studies & ResearchPrachya Adhyayan
 
RCPE terms and cycles scenarios as of March 2024
RCPE terms and cycles scenarios as of March 2024RCPE terms and cycles scenarios as of March 2024
RCPE terms and cycles scenarios as of March 2024suelcarter1
 
Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...
Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...
Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...Sérgio Sacani
 
Alternative system of medicine herbal drug technology syllabus
Alternative system of medicine herbal drug technology syllabusAlternative system of medicine herbal drug technology syllabus
Alternative system of medicine herbal drug technology syllabusPradnya Wadekar
 
Role of Herbs in Cosmetics in Cosmetic Science.
Role of Herbs in Cosmetics in Cosmetic Science.Role of Herbs in Cosmetics in Cosmetic Science.
Role of Herbs in Cosmetics in Cosmetic Science.ShwetaHattimare
 
geometric quantization on coadjoint orbits
geometric quantization on coadjoint orbitsgeometric quantization on coadjoint orbits
geometric quantization on coadjoint orbitsHassan Jolany
 
PSP3 employability assessment form .docx
PSP3 employability assessment form .docxPSP3 employability assessment form .docx
PSP3 employability assessment form .docxmarwaahmad357
 
Substances in Common Use for Shahu College Screening Test
Substances in Common Use for Shahu College Screening TestSubstances in Common Use for Shahu College Screening Test
Substances in Common Use for Shahu College Screening TestAkashDTejwani
 
biosynthesis of the cell wall and antibiotics
biosynthesis of the cell wall and antibioticsbiosynthesis of the cell wall and antibiotics
biosynthesis of the cell wall and antibioticsSafaFallah
 
IB Biology New syllabus B3.2 Transport.pptx
IB Biology New syllabus B3.2 Transport.pptxIB Biology New syllabus B3.2 Transport.pptx
IB Biology New syllabus B3.2 Transport.pptxUalikhanKalkhojayev1
 
001 Case Study - Submission Point_c1051231_attempt_2023-11-23-14-08-42_ABS CW...
001 Case Study - Submission Point_c1051231_attempt_2023-11-23-14-08-42_ABS CW...001 Case Study - Submission Point_c1051231_attempt_2023-11-23-14-08-42_ABS CW...
001 Case Study - Submission Point_c1051231_attempt_2023-11-23-14-08-42_ABS CW...marwaahmad357
 
CW marking grid Analytical BS - M Ahmad.docx
CW  marking grid Analytical BS - M Ahmad.docxCW  marking grid Analytical BS - M Ahmad.docx
CW marking grid Analytical BS - M Ahmad.docxmarwaahmad357
 
MARSILEA notes in detail for II year Botany.ppt
MARSILEA  notes in detail for II year Botany.pptMARSILEA  notes in detail for II year Botany.ppt
MARSILEA notes in detail for II year Botany.pptaigil2
 
Main Exam Applied biochemistry final year
Main Exam Applied biochemistry final yearMain Exam Applied biochemistry final year
Main Exam Applied biochemistry final yearmarwaahmad357
 
Q3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptx
Q3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptxQ3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptx
Q3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptxArdeniel
 
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdfSUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdfsantiagojoderickdoma
 
Application of Foraminiferal Ecology- Rahul.pptx
Application of Foraminiferal Ecology- Rahul.pptxApplication of Foraminiferal Ecology- Rahul.pptx
Application of Foraminiferal Ecology- Rahul.pptxRahulVishwakarma71547
 

Dernier (20)

Applying Cheminformatics to Develop a Structure Searchable Database of Analyt...
Applying Cheminformatics to Develop a Structure Searchable Database of Analyt...Applying Cheminformatics to Develop a Structure Searchable Database of Analyt...
Applying Cheminformatics to Develop a Structure Searchable Database of Analyt...
 
Exploration Method’s in Archaeological Studies & Research
Exploration Method’s in Archaeological Studies & ResearchExploration Method’s in Archaeological Studies & Research
Exploration Method’s in Archaeological Studies & Research
 
RCPE terms and cycles scenarios as of March 2024
RCPE terms and cycles scenarios as of March 2024RCPE terms and cycles scenarios as of March 2024
RCPE terms and cycles scenarios as of March 2024
 
Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...
Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...
Digitized Continuous Magnetic Recordings for the August/September 1859 Storms...
 
Alternative system of medicine herbal drug technology syllabus
Alternative system of medicine herbal drug technology syllabusAlternative system of medicine herbal drug technology syllabus
Alternative system of medicine herbal drug technology syllabus
 
Role of Herbs in Cosmetics in Cosmetic Science.
Role of Herbs in Cosmetics in Cosmetic Science.Role of Herbs in Cosmetics in Cosmetic Science.
Role of Herbs in Cosmetics in Cosmetic Science.
 
geometric quantization on coadjoint orbits
geometric quantization on coadjoint orbitsgeometric quantization on coadjoint orbits
geometric quantization on coadjoint orbits
 
PSP3 employability assessment form .docx
PSP3 employability assessment form .docxPSP3 employability assessment form .docx
PSP3 employability assessment form .docx
 
Substances in Common Use for Shahu College Screening Test
Substances in Common Use for Shahu College Screening TestSubstances in Common Use for Shahu College Screening Test
Substances in Common Use for Shahu College Screening Test
 
Data delivery from the US-EPA Center for Computational Toxicology and Exposur...
Data delivery from the US-EPA Center for Computational Toxicology and Exposur...Data delivery from the US-EPA Center for Computational Toxicology and Exposur...
Data delivery from the US-EPA Center for Computational Toxicology and Exposur...
 
biosynthesis of the cell wall and antibiotics
biosynthesis of the cell wall and antibioticsbiosynthesis of the cell wall and antibiotics
biosynthesis of the cell wall and antibiotics
 
IB Biology New syllabus B3.2 Transport.pptx
IB Biology New syllabus B3.2 Transport.pptxIB Biology New syllabus B3.2 Transport.pptx
IB Biology New syllabus B3.2 Transport.pptx
 
001 Case Study - Submission Point_c1051231_attempt_2023-11-23-14-08-42_ABS CW...
001 Case Study - Submission Point_c1051231_attempt_2023-11-23-14-08-42_ABS CW...001 Case Study - Submission Point_c1051231_attempt_2023-11-23-14-08-42_ABS CW...
001 Case Study - Submission Point_c1051231_attempt_2023-11-23-14-08-42_ABS CW...
 
CW marking grid Analytical BS - M Ahmad.docx
CW  marking grid Analytical BS - M Ahmad.docxCW  marking grid Analytical BS - M Ahmad.docx
CW marking grid Analytical BS - M Ahmad.docx
 
MARSILEA notes in detail for II year Botany.ppt
MARSILEA  notes in detail for II year Botany.pptMARSILEA  notes in detail for II year Botany.ppt
MARSILEA notes in detail for II year Botany.ppt
 
Main Exam Applied biochemistry final year
Main Exam Applied biochemistry final yearMain Exam Applied biochemistry final year
Main Exam Applied biochemistry final year
 
Q3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptx
Q3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptxQ3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptx
Q3W4part1-SSSSSSSSSSSSSSSSSSSSSSSSCI.pptx
 
Cheminformatics tools and chemistry data underpinning mass spectrometry analy...
Cheminformatics tools and chemistry data underpinning mass spectrometry analy...Cheminformatics tools and chemistry data underpinning mass spectrometry analy...
Cheminformatics tools and chemistry data underpinning mass spectrometry analy...
 
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdfSUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
SUKDANAN DIAGNOSTIC TEST IN PHYSICAL SCIENCE ANSWER KEYY.pdf
 
Application of Foraminiferal Ecology- Rahul.pptx
Application of Foraminiferal Ecology- Rahul.pptxApplication of Foraminiferal Ecology- Rahul.pptx
Application of Foraminiferal Ecology- Rahul.pptx
 

It's all about Science!

  • 2.  1 resealable plastic bag  Strawberries (fresh or frozen)  2 teaspoons of dish detergent  1 teaspoon of salt  ½ cup of deionized water  2 plastic cups  Filtering apparatus  Ice cold 90 percent rubbing alcohol  1 wooden popsicle stick or plastic coffee stirrer  Gloves  1 vial
  • 3.  Pull off any green leaves on the strawberries.  Put some strawberries into the plastic bag, remove the air and seal it.  Gently smash the strawberries and crush them completely. This starts to break open the cells and release the DNA.
  • 5.  In a plastic cup make your buffer solution: mix together the deionized water, the salt and the detergent.  Add 2 teaspoons of the buffer solution into the bag with the strawberries. This will further break open the cells.  Reseal the bag and continue smashing the strawberries gently for a while.
  • 7.  Place the coffee filter inside the other plastic cup.  Open the bag and pour the strawberry liquid into the filter. You can gently squeeze the remaining liquid into the cup.  Put approximately 4 ml of the liquid into the vial.
  • 9.  Tilt the vial a little bit and then pour slow and carefully about 16 ml of cold rubbing alcohol (until it reaches 20 ml). Do NOT mix or stir. You have just isolated the DNA from the rest of the material contained in the cells of the strawberry.  Within a few seconds, watch for the development of a white cloudy substance (DNA) in the top layer above the strawberry extract layer.  Pick up the DNA using a plastic coffee stirrer or a wooden stick.
  • 11. This is your DNA…
  • 13. You can also save the DNA you extracted into a small vial containing alcohol. In the vial with the strawberry liquid and the alcohol, DNA will continue appearing and if you shake it a little bit, it will have a smooth reddish color.
  • 15. You can keep both vials in normal atmosphere conditions without damaging the DNA. Just have in mind to change the alcohol after a few years!