Presentation held at the Innovation Forum 2009 with topic microfluidic applications. The presentation gives a view of the broad range of technologies and project methodologies required when developing fully integrated microfluidic devices or lab-on-chips.

1. Bringing microFluidics to Life
Dr.ir. Maurice van der Beek, Dr. Eduard Pelssers
Philips Applied Technologies
Micro Technology Innovation Forum
January 21, 2009

2. Philips Applied Technologies
A leading technology center
Founded in 1968
Sales 2007
EUR 149 Million
Worldwide representation
North America: San José (USA), Andover (USA)
Europe: Eindhoven (NL), Redhill (UK)
Asia: Bangalore (India), Singapore
University degree / Ph.D.s Other type of education
± 390 ± 140 A multinational highly qualified workforce of about
845 people (+ 150 temporary employees)
Bachelors & Engineers
± 315
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 2

3. Contract R&D services
Innovation through turnkey solutions and specialist support
Healthcare Lifestyle Technology
• Medical devices & implants • Multimedia Experience • Home and Building
• Patient monitoring & • Personal Care, Wellness & Automation & Security
connected care Beauty • Instrumentation & Stages
• Medical imaging & therapy • Robotics • Opto-electronic modules
systems • Information, Storage & • RFID
• Molecular healthcare devices Streaming & Retrieval • Energy
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 3

4. R&D for every phase of the innovation process
Concept Design Engineering Industrialization Ramp-up
• Research • Product • Technical • Prototypes • Manufacturing
design Product Docu- support
• Innovation • Industry
consulting • Process mentation
(TPD) consulting • Small volume
design production
• Equipment • Functional
design models
• Quality and
reliability
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 4

5. Customers
Philips Consumer Lifestyle
Philips Lighting
Philips Healthcare
Philips Research
Philips Incubators
Philips IP&S
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 5

6. Magnotech - Cozart and Philips
Cozart (a wholly owned subsidiary of Concateno plc) and Philips are engaged to develop
and commercialize an innovative new handheld drugs-of-abuse testing product based on
Magnotech™ technology
Rapid Drug Screen with
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 6

7. Magnotech - Platform Elements
Disposable Analyser
• Sample taking unit • Magnetic actuation
• Pre-treatment • Signal processing
• Detection • No moving parts
• Embedded software
Bio-chemistry Software
• Magnetic beads • Host software
• Printed spots • Integration with clinical
• Dry reagents IT infrastructure
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 7

8. What is needed: system architecture approach
Disposable
• Sample taking unit
• Pre-treatment
product requirements Low cost / mass manufacturable • Detection
Reaction vessel enabling all processes
functional for the detection of drugs from saliva
architecture
Sample taking Pre-treatment Sample transport Labeling Enabling label detection
architecture Cotton swab Filtering Capillary force Immuno-magnetic SPR
options Paper Chemical Pressure difference Fluorescent Optical
Pipette Enzymatic Electrowetting None Magnetic
etc. etc. etc. etc. Etc.
system
architecture Product concepts
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 8

9. What is needed: a multidisciplinary team
Biochemistry
Mechanics
Transport
(Colloid) phenomena
Chemistry
Physics Optics
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 9

10. What is needed: a structured way-of-working
Stargate – a lifecycle model for business creation
Concept Design Engineering Industrialization Ramp-up
• ?
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 10

11. What is needed: access to techniques for integration
Microfluidic design
Plastics molding
Joining / laser Microfluidic
welding functional testing
Pre-pilot Assembly
Pilot Production
Surface
modification
Bio
spotting
Assay development
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 11

12. Microfluidic design and functional testing
CHALLENGE
• Realization of microfluidic fluid
handling steps realized without
user actuation (autonomous flow)
RESULT
• Sample treatment, aliquoting, and
rapid transport based on capillary
flow
• Realization of an evaluation tool 45
40
35
30
BENEFITS
Spot nr.
25
• Fully autonomous 20
15
• Cost effective implementation in 10
disposables 5
• General use – Microfluidic Platform 0
0 0.5 1 1.5 2 2.5 3
time (s)
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 12

13. Micro injection molding
CHALLENGE
• Mass manufacturing of plastics featuring
submillimeter structures for use in
microfluidic applications
RESULT
• Mold insert creation using various
techniques, i.e. micro-milling, micro-EDM,
laser ablation, focused ion beam,
diamond tip milling
• Replication of micrometer-sized features
into plastics using the process of injection
molding
BENEFITS picture
• Design freedom
• Dimensional accuracy
• High degree of automation
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 13

14. Laser welding of biomedical cartridges
CHALLENGE
• Hermetic sealing of (transparent) polymer cartridges for
biomedical applications
RESULT
• Thermal joining of parts using standard IR fiberlasers.
• Absorption of laser light by addition of carbon black or by
polymer itself dependent on wavelength.
Top view of cartridge
BENEFITS fluid inlet
• High quality joint for polymer parts
laser weld
• No addition of chemicals is required
• Accurate and flexible technique.
• Small heat affected zone; no adverse
channel
effect to pre-immobilized antibodies
filled with
fluid
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 14

15. Deposition and localization of protein activated magnetic
particles
CHALLENGE
• Deposition of small volumes (typical 100 nl)
of a liquid with relative high viscosity
containing protein coated magnetic
particles with high precision of both
volume and location
RESULT
• Selection and modification of existing
dosing system by extending both hardware
(vision) and software of the instrument
BENEFITS
• Cost effective development of equipment
for small scale production with a clear open
route towards larger scale production
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 15

16. Pilot-line production in clean room
CHALLENGE
• Bio-compatible assembly of plastic micro-fluidic
products
RESULT
• Micro-biological clean manufacturing:
• Cross-flow and down-flow boxes
• Clean room class 10000
• Fixed instructions, product tracking
• Automated visual inspection
BENEFITS
• Scalable production
Confidential Philips Applied Technologies, Author, MMMM dd, yyyy, Reference
Philips Applied Technologies, Maurice van der Beek, January 21 2009, Micro Technology Innovation Forum 16