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B ZERO
AFLA B1
48 det. format
96 det. format

MA221
MA220

48 det. format
96 det. format

ELISA tools for the quantitative detection of
aflatoxin B1 in cereals, feed, nuts, spices
www.tecnalab.com

MA222
MA224
PERFORMANCES



Quickness



High sensitivity, in compliance
with worldwide requirements
for food and feedstuffs



High accuracy



High reproducibility



Cost-effectivness
without
forgoing
robustness,
accuracy and precision



Development of an ELISA test
kit format suitable for those
implementing a few samples
per session with no necessity
of installing a lateral flow
working
station
(no
dedicated reader)

Ease of use



ECONOMY

www.tecnalab.com
B ZERO
AFLA B1

•

15 minutes assays for the quantitative detection of aflatoxin B1 in
cereals, high moisture maize, silage, mash, feed, nuts, dried fruits, DDGS,
cottonseeds

•

Detection limit: 1 ppb aflatoxin B1

•

Specificity of the assays:
•Aflatoxin B1 100%
•Aflatoxin B2 5%
•Aflatoxin G1 19%
•Aflatoxin G2 < 1%

•

www.tecnalab.com

Sample preparation: grinding, extraction in methanol-water, filtration
(EasyPur purification for cottonseeds) For silage and mash pH
adjustment of the methanol extracts is advisable.
MAIZE Performances
SPECIFICITY

SENSITIVITY

Cut Off < 1 ppb

LOQ 1 ppb

materials left over FAPAS proficiency tests

PROFICIENCY
TEST RESULTS

Proficiency

C1 (maize)

Assigned AfB1 value (ppb)

Mean result (ppb)

T0497

3.9 (sat. range 2.2 – 5.6)

4.3 + 0.2

T0473

4.7 (sat. range 2.6 – 6.7)

5.8 + 0.8

5.2 (sat. range 2.3 – 7.5)

5.8 + 0.5

Assigned value (ppb)

Celer AFLA B1 result (ppb)

Z-Score

AIA 2008

0.03

< 1.00

Satisfactory

C2 (maize)

AIA 2008

36.85

43.67

0.63

C3 (maize)

AIA 2008

19.36

22.99

0.73

C4 (maize)

AIA 2008

11.41

13.39

0.62

C1 (maize)

AIA 2009

10.81

9.90

-0.30

C2 (maize)

AIA 2009

36.97

37.29

0.02

C3 (maize)

AIA 2009

20.78

20.18

-0.09

C4 (maize)

AIA 2009

0.10

< 1.00

Satisfactory

C1 (maize)

AIA 2010

0.13

< 1.00

Satisfactory

C2 (maize)

AIA 2010

5.74

7.55

0.78

C3 (maize)

AIA 2010

10.96

13.30

0.43

C4 (maize)
www.tecnalab.com

Sample cod.

Code

T04105

TRUENESS assessed on reference

AIA 2010

24.38

24.61

0.02

MA1350-1 (maize)

PTS 2013

1.59

1.27

-0.48

MA1350-2 (maize)

PTS 2013

2.61

1.85

-0.75
FEED Performances

Code

materials left over FAPAS proficiency tests

PRECISION

Assigned AfB1 value (ppb)

Mean result (ppb)

T0491

11.3 (sat. range 6.4 – 16.3)

11.9 + 1.8

T04102

18.1 (sat. range 10.2 – 26.1)

15.4 + 0.9

T0486

TRUENESS assessed on reference

23.0 (sat. range 12.8 – 33.1)

24.8 + 2.5

Code

Intra-assay CV (%), n = 9

Inter-assay CV (%) n = 3

T0491

5.2

12.1

T04102

2.8

3.9

T0486

6.1

2.0

Intra-assay CV was assessed by running 3 times the sampe sample in 3 sessions.
Inter-assay CV was assessed by analysing the samples in 3 different days.

www.tecnalab.com
NUTS & SPICES Performances
SPECIFICITY (Cut Off)

Hazelnuts

< 1 ppb

Pistachio nut < 1 ppb
Raisins
Figs

SENSITIVITY (LOQ)

< 1 ppb
< 1 ppb

Hazelnuts

1 ppb

Pistachio nut 1 ppb
Raisins

2 ppb

Figs

2 ppb

TRUENESS assessed on reference
materials left over FAPAS proficiency tests
Code

Assigned AfB1 value (ppb)

Mean result (ppb)

T0484

Peanut

1.54 (sat. range 0.7 – 2.2)

1.4 + 0.9

T0483

www.tecnalab.com

Type
Fig

4.4 (sat. range 2.4 – 6.3)

4.4 + 0.0
Why a MASTER-CURVE version of Celer AFLA B1?
Cost comparison in terms of wells when running 3 samples in a session

COMMON ELISA KIT

B ZERO TEST KIT

Zero standard

Zero standard

Standard 1

SAMPLE 1

Standard 2

SAMPLE 2

Standard 3

SAMPLE 3
Total expence: 4 wells

Standard 4
SAMPLE 1
SAMPLE 2
SAMPLE 3

Calculate the B/Bo of each
sample and interpolate
onto the provided virtual
curves to find the
concentration.

Total expence: 8 wells
RAFA 2013
B ZERO
AFLA B1
has been compared in terms of
performances on maize screening
to Celer AFLA B1.
(Effects of “master curve calibrations”
on the performances of some ELISAs for
food contaminants, Rosar et al, 2013,
poster presentation @ Recent
Advances In Food Analysis symposium,
4-9 Nov 2013, Prague, Czech Republic)
B ZERO
MAIZE Performances
AFLA B1
SPECIFICITY

SENSITIVITY

Cut Off < 1 ppb

LOQ 2 ppb

TRUENESS
assessed on a Reference
Material left over FAPAS proficiency tests

Code

Assigned AfB1 value (ppb)

Mean result (ppb)

T0497

3.9 (sat. range 2.2 – 5.6)

4.2 + 0.3

on a Trilogy Reference Material

Code

Assigned AfB1 value (ppb)

Mean result (ppb)

18.8

16.5 + 0.6

Assigned AfB1 value (ppb)

Mean result (ppb)

MA1350-1

1.6 (sat. range 0.2 – 2.9)

1.5 + 0.2

MA1350-2

2.6 (sat. range 0.6 - 4.6)

2.0 + 0.2

TR_MTC9999D

and on two Progetto Trieste Reference
Materials

www.tecnalab.com

Code
B ZERO
AFLA B1
ALL GOALS FULFILLED

Quickness, easiness, high
specificity and sensitivity were
obtained.
Trueness was high and
proficiency tests z-scores were
always satisfactory.

www.tecnalab.com

ALL GOALS FULFILLED

Cost-effectiveness was
obtained without forgoing
robustness, accuracy and
precision in a suitable format for
costumers implementing a few
samples per session.
More…
Tecna’s port-folio of rapid and sensitive tools for mycotoxin
screening in cereals

B ZERO
Available for aflatoxin B1, deoxynivalenol and zearalenone NEW!

available for total aflatoxins, aflatoxin B1, fumonisins,
zearalenone, deoxynivalenol, T2/HT2 toxins
available for aflatoxins, ochratoxin A and fumonisins NEW!

SMART STRIP

NEW quantitative lateral flow for deoxynivalenol
SOON AVAILABLE for aflatoxin B1
www.tecnalab.com

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Reliable analysis of aflatoxin B1

  • 1. B ZERO AFLA B1 48 det. format 96 det. format MA221 MA220 48 det. format 96 det. format ELISA tools for the quantitative detection of aflatoxin B1 in cereals, feed, nuts, spices www.tecnalab.com MA222 MA224
  • 2. PERFORMANCES  Quickness  High sensitivity, in compliance with worldwide requirements for food and feedstuffs  High accuracy  High reproducibility  Cost-effectivness without forgoing robustness, accuracy and precision  Development of an ELISA test kit format suitable for those implementing a few samples per session with no necessity of installing a lateral flow working station (no dedicated reader) Ease of use  ECONOMY www.tecnalab.com
  • 3. B ZERO AFLA B1 • 15 minutes assays for the quantitative detection of aflatoxin B1 in cereals, high moisture maize, silage, mash, feed, nuts, dried fruits, DDGS, cottonseeds • Detection limit: 1 ppb aflatoxin B1 • Specificity of the assays: •Aflatoxin B1 100% •Aflatoxin B2 5% •Aflatoxin G1 19% •Aflatoxin G2 < 1% • www.tecnalab.com Sample preparation: grinding, extraction in methanol-water, filtration (EasyPur purification for cottonseeds) For silage and mash pH adjustment of the methanol extracts is advisable.
  • 4. MAIZE Performances SPECIFICITY SENSITIVITY Cut Off < 1 ppb LOQ 1 ppb materials left over FAPAS proficiency tests PROFICIENCY TEST RESULTS Proficiency C1 (maize) Assigned AfB1 value (ppb) Mean result (ppb) T0497 3.9 (sat. range 2.2 – 5.6) 4.3 + 0.2 T0473 4.7 (sat. range 2.6 – 6.7) 5.8 + 0.8 5.2 (sat. range 2.3 – 7.5) 5.8 + 0.5 Assigned value (ppb) Celer AFLA B1 result (ppb) Z-Score AIA 2008 0.03 < 1.00 Satisfactory C2 (maize) AIA 2008 36.85 43.67 0.63 C3 (maize) AIA 2008 19.36 22.99 0.73 C4 (maize) AIA 2008 11.41 13.39 0.62 C1 (maize) AIA 2009 10.81 9.90 -0.30 C2 (maize) AIA 2009 36.97 37.29 0.02 C3 (maize) AIA 2009 20.78 20.18 -0.09 C4 (maize) AIA 2009 0.10 < 1.00 Satisfactory C1 (maize) AIA 2010 0.13 < 1.00 Satisfactory C2 (maize) AIA 2010 5.74 7.55 0.78 C3 (maize) AIA 2010 10.96 13.30 0.43 C4 (maize) www.tecnalab.com Sample cod. Code T04105 TRUENESS assessed on reference AIA 2010 24.38 24.61 0.02 MA1350-1 (maize) PTS 2013 1.59 1.27 -0.48 MA1350-2 (maize) PTS 2013 2.61 1.85 -0.75
  • 5. FEED Performances Code materials left over FAPAS proficiency tests PRECISION Assigned AfB1 value (ppb) Mean result (ppb) T0491 11.3 (sat. range 6.4 – 16.3) 11.9 + 1.8 T04102 18.1 (sat. range 10.2 – 26.1) 15.4 + 0.9 T0486 TRUENESS assessed on reference 23.0 (sat. range 12.8 – 33.1) 24.8 + 2.5 Code Intra-assay CV (%), n = 9 Inter-assay CV (%) n = 3 T0491 5.2 12.1 T04102 2.8 3.9 T0486 6.1 2.0 Intra-assay CV was assessed by running 3 times the sampe sample in 3 sessions. Inter-assay CV was assessed by analysing the samples in 3 different days. www.tecnalab.com
  • 6. NUTS & SPICES Performances SPECIFICITY (Cut Off) Hazelnuts < 1 ppb Pistachio nut < 1 ppb Raisins Figs SENSITIVITY (LOQ) < 1 ppb < 1 ppb Hazelnuts 1 ppb Pistachio nut 1 ppb Raisins 2 ppb Figs 2 ppb TRUENESS assessed on reference materials left over FAPAS proficiency tests Code Assigned AfB1 value (ppb) Mean result (ppb) T0484 Peanut 1.54 (sat. range 0.7 – 2.2) 1.4 + 0.9 T0483 www.tecnalab.com Type Fig 4.4 (sat. range 2.4 – 6.3) 4.4 + 0.0
  • 7. Why a MASTER-CURVE version of Celer AFLA B1? Cost comparison in terms of wells when running 3 samples in a session COMMON ELISA KIT B ZERO TEST KIT Zero standard Zero standard Standard 1 SAMPLE 1 Standard 2 SAMPLE 2 Standard 3 SAMPLE 3 Total expence: 4 wells Standard 4 SAMPLE 1 SAMPLE 2 SAMPLE 3 Calculate the B/Bo of each sample and interpolate onto the provided virtual curves to find the concentration. Total expence: 8 wells RAFA 2013
  • 8. B ZERO AFLA B1 has been compared in terms of performances on maize screening to Celer AFLA B1. (Effects of “master curve calibrations” on the performances of some ELISAs for food contaminants, Rosar et al, 2013, poster presentation @ Recent Advances In Food Analysis symposium, 4-9 Nov 2013, Prague, Czech Republic)
  • 9. B ZERO MAIZE Performances AFLA B1 SPECIFICITY SENSITIVITY Cut Off < 1 ppb LOQ 2 ppb TRUENESS assessed on a Reference Material left over FAPAS proficiency tests Code Assigned AfB1 value (ppb) Mean result (ppb) T0497 3.9 (sat. range 2.2 – 5.6) 4.2 + 0.3 on a Trilogy Reference Material Code Assigned AfB1 value (ppb) Mean result (ppb) 18.8 16.5 + 0.6 Assigned AfB1 value (ppb) Mean result (ppb) MA1350-1 1.6 (sat. range 0.2 – 2.9) 1.5 + 0.2 MA1350-2 2.6 (sat. range 0.6 - 4.6) 2.0 + 0.2 TR_MTC9999D and on two Progetto Trieste Reference Materials www.tecnalab.com Code
  • 10. B ZERO AFLA B1 ALL GOALS FULFILLED Quickness, easiness, high specificity and sensitivity were obtained. Trueness was high and proficiency tests z-scores were always satisfactory. www.tecnalab.com ALL GOALS FULFILLED Cost-effectiveness was obtained without forgoing robustness, accuracy and precision in a suitable format for costumers implementing a few samples per session.
  • 11. More… Tecna’s port-folio of rapid and sensitive tools for mycotoxin screening in cereals B ZERO Available for aflatoxin B1, deoxynivalenol and zearalenone NEW! available for total aflatoxins, aflatoxin B1, fumonisins, zearalenone, deoxynivalenol, T2/HT2 toxins available for aflatoxins, ochratoxin A and fumonisins NEW! SMART STRIP NEW quantitative lateral flow for deoxynivalenol SOON AVAILABLE for aflatoxin B1 www.tecnalab.com