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BT-202 Netaji Subhas Institute of Technology, Dwarka, New Delhi. Dr. Amita Pandey Aug 26, 2011
Learning check! ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Structure of proteins Proteins perform diverse functions in the cell.
Primary Structure ,[object Object],[object Object],[object Object],[object Object]
Polymorphic proteins ,[object Object],[object Object],[object Object]
First protein Sequence ,[object Object],[object Object],[object Object]
End Group Analysis ,[object Object],[object Object],[object Object],[object Object]
End Group Analysis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Edman degradation ,[object Object],[object Object],[object Object],[object Object]
Sequencing larger proteins ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Breaking disulfide bond ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Breaking disulfide bond ,[object Object],[object Object],[object Object],[object Object]
Enzymatic and Chemical Cleavage ,[object Object],[object Object],[object Object],[object Object]
peptide fragments Sequencing Edman procedure Ordering of peptides Cleaved again with different enzyme or  chemical
 
Learning check ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Protein sequencing ,[object Object],[object Object],[object Object],[object Object]
Genome sequencing organism Size of genome Year  Haemophilus  1.8 Mb 1995 Saccharomyces 12.1 Mb 1996 C. elegans 100Mb 1998 Fruit fly 139 Mb 2005 Human 3.5 Gb 2006
Consensus sequences
Synthesis of peptides Three  ways to obtain a peptide are -from the tissue -genetic engineering -chemical synthesis
Chemical synthesis 9-fluorenylmethoxycarbonyl
 
Proteins ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Protein folding ,[object Object],[object Object]
Non-covalent bonds in protein folding
Globular protein
Hydrogen Bonds in Proteins
Protein folding ,[object Object],[object Object],[object Object],[object Object]
Protein folding ,[object Object],[object Object],[object Object]
Protein folding  -helix – protein turns like a spiral – fibrous proteins (hair, nails, horns)  -sheet – protein folds back on  itself as in a ribbon –globular protein
   Sheets ,[object Object],[object Object],[object Object],[object Object],[object Object]
   Helix ,[object Object],[object Object],[object Object],[object Object]
Protein structure
Domains ,[object Object],[object Object],[object Object],[object Object]
Domains
Protein Families ,[object Object],[object Object]
Proteins – Multiple Peptides ,[object Object],[object Object],[object Object],[object Object],[object Object]
Single Subunit Proteins
Different Subunit Proteins ,[object Object],[object Object],[object Object]
Protein Assemblies ,[object Object],[object Object],[object Object]
Types of Proteins ,[object Object],[object Object],[object Object],[object Object],[object Object]
Important Fibrous Proteins ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Collagen and Elastin
Stabilizing Cross-Links ,[object Object],[object Object]
Proteins at Work ,[object Object],[object Object],[object Object],[object Object]
Ligand Binding
Formation of Binding Site ,[object Object],[object Object]
Antibody Family ,[object Object],[object Object],[object Object]
Antibodies ,[object Object],[object Object],[object Object]
Antibodies
Binding Strength ,[object Object],[object Object],[object Object],[object Object]
Equilibrium Constant ,[object Object],[object Object]
Enzymes as Catalysts ,[object Object],[object Object],[object Object],[object Object],[object Object]
Enzymes at Work ,[object Object],[object Object],[object Object],[object Object]
Lysozyme ,[object Object]
Features of Enzyme Catalysis
 

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Aug 26 2011