1. Squamous
Cell Lung
Carcinoma
Looking for
tell-tale
Nacho
signs Caballero

2. P2-N

3. P1 P2
P4 P3

4. Normal
P2
Intermediate Tumor

5. P2-N

6. Human height follows
a normal distribution

7. Distribution of the number of reads
that align to each gene
# reads

8. RNA Seq data
can be modeled using a
negative binomial distribution
# reads

9. Significance
High
Low
- 0 +
Log Fold Change

10. Genes with significant
count differences between
normal and intermediate samples
Normal
Intermediate
Tumor
Patient 1
Patient 2
Patient 3
Patient 4
100K counts
10K counts
1K counts
100 counts
10 counts
1 counts

11. CDON counts are significantly smaller
in intermediate than in normal
Patient 1
Patient 2
Patient 3
Patient 4

12. Number of significant genes
71 1267
Intermediate Tumor
vs vs
Normal Normal

13. Number of significant genes
4 patients 3 patients
4 patients 3 patients
43 28 15 226 1041 217
Intermediate Tumor
vs vs
Normal Normal

14. Interpreting
the tell-tale
signs