SlideShare a Scribd company logo
1 of 33
NEMATODES: A LABORATORY
PERSPECTIVE
GENERAL CHARACTERISTICS OF NEMATODES

   They assume three basic morphologic
   forms: Egg, Larvae and Adult worms



   Reproduce sexually with male and
   female worms


   Complete digestive tract and
   complete reproductive systems
TAXONOMIC CLASSIFICATION
                             Intestinal Species

                                 E. vermicularis
                                 T. trichura
                                 N. americanus
                                 A. duodenale
Phylum                           A. lumbricoides
                  Class          S. stercoralis
Nemathelminthes
                  Nematoda


                             Intestinal- Tissue
                             Species

                                 T. spiralis
                                 D. medinensis
ASCARIS LUMBRICOIDES




                        Ascaris lumbricoides
        Common Name          Giant Intestinal Roundworm
         Infective Stage     Embryonated Egg
             Habitat         Small Intestine
      Mode of Transmission   Ingestion of contaminated food/ water
      Diagnostic Specimen    Feces
ASCARIS LUMBRICOIDES 2




           A. lumbricoides Adult worms
ENTEROBIUS VERMICULARIS




                        Enterobius vermicularis
     Common Name        Pinworm
      Infective Stage   Embryonated Egg
          Habitat       Large Intestine
   Mode of Transmission Ingestion of egg/ autoinfection
   Diagnostic Specimen Feces/ Cellophane Tape prep
TRICHURIS TRICHURA




                             Trichuris trichura
     Common Name        Whipworm
      Infective Stage   Embryonated Egg
          Habitat       Large Intestine
   Mode of Transmission Ingestion of egg via contaminated food/ water
   Diagnostic Specimen Feces
TRICHURIS TRICHURA 2
NECATOR AMERICANUS AND ANCYLOSTOMA
DUODENALE




                 Necator americanus and Ancylostoma duodenale
  Common Name         Hookworm
   Infective Stage    Third Stage Filariform Larvae
       Habitat        Small Intestine
Mode of Transmission Skin Penetration of Infective Larvae
Diagnostic Specimen Feces
HOOKWORMS 2




    A. duodenale   N. americanus
STRONGYLOIDES STERCORALIS




                            Strongyloides stercoralis
   Common Name        Threadworm
    Infective Stage   Third Stage Filariform Larvae
        Habitat       duodenum and upper jejunum
 Mode of Transmission Skin Penetration of Infective Larvae/ autoinfection
 Diagnostic Specimen Feces
TRICHINELLA SPIRALIS




                                  Trichinella spiralis
      Common Name        Trichinia worm
       Infective Stage   Encysted Larvae
           Habitat       Striated muscle tissue
          Mode of
        Transmission    Ingestion of raw/ undercooked contaminated meat
    Diagnostic Specimen skeletal muscle biopsy/ Blood (LDH, Aldolase, CPK, eo ct)
CAPILLARIA PHILIPPINENSIS




                                Capillaria philippinensis
     Common Name          NA
      Infective Stage     Encysted Larvae
          Habitat         Small Intestine
   Mode of Transmission   Ingestion of raw/ undercooked contaminated fish
   Diagnostic Specimen    Feces
THE FILARIAE- GENERAL CHARACTERISTICS
  Two morphologic forms: Adult worms and
  Microfilariae

  Adult worms are usually creamy
  white and assume a filariform shape

  Have both male and female
  forms
  Females lay live microfliariae

  Require arthropod vectors

  Exhibit periodicity
  (Nocturnal/ diurnal)
WUCHERERIA BANCROFTI




                                 Wuchereria bancrofti
    Common Name        Bancroft's Filaria
     Infective Stage   L3 Larva
         Habitat       Lymphatics and blood
  Mode of Transmission Bite from infected mosquito (Culex, Aedes or Anopheles)
  Diagnostic Specimen Giemsa stained smear (collected at night)/ Knott's Technique
BRUGIA MALAYI




                                     Brugia malayi
   Common Name        Malayan Filaria
    Infective Stage   L3 Larva
        Habitat       Lymphatics and Blood
                      Bite from infected mosquito ( Anopheles, Aedes, Mansonia,
 Mode of Transmission Amigeres)
 Diagnostic Specimen Giemsa stained smear (collected at night)/ Knott's Technique
WUCHERERIA BANCROFTI V.S. BRUGIA MALAYI

       Distinguishing         Wuchereria            Brugia malayi
       Characteristics         bancrofti
      Sheath in Giemsa         unstained                 Pink
           Nuclei           Regularly spaced,     Irregularly spaced
                           separately situated     and overlapping
            Tail                  none           Single row of nuclei
                                                 that reach tail’s end
       Terminal Nuclei           None               2 nuclei, which
                                                   bulge the cuticle;
                                                     conspicuously
                                                        placed
     Appearance in Blood    Smoothly curved              Kinky
            film
LOA LOA




                                        Loa loa
  Common Name       African Eye Worm
  Infective Stage   L3 Larva
      Habitat       Subcutaneous tissue, the eye and bridge of nose
      Mode of
   Transmission     Bite from infected Chrysops silacea or C. dimidiata (Deer Fly)
    Diagnostic      Giemsa stained smear (collected at midday 10:15to 2:15)/ Knott's
     Specimen       Technique
ONCHOCERCA VOLVULUS




                             Onchocerca volvulus
     Common Name        Blinding Filaria
      Infective Stage   L3 Larva
          Habitat       Subcutaneous tissue, nodules and eyes
   Mode of Transmission Bite from infected Simulium Blackfly
   Diagnostic Specimen Giemsa stained smear of skin snips
MANSONELLA OZZARDI AND MANSONELLA
PERSTANS




                        Mansonella ozzardi and Mansonella perstans
   Common Name Ozzard's Filaria and Perstans Filaria
    Infective Stage   L3 Larva
        Habitat       Blood and various tissue
 Mode of Transmission Bite from infected Simulium Blackfly or Culicoides (sucking midge fly)
 Diagnostic Specimen Fresh Giemsa stained blood smear
Long Quiz next meeting?




                             The End
PART 2: KATO-KATZ, KATO-THICK AND
CONCENTRATION TECHNIQUES
KATO KATZ TECHNIQUE




                      Step 2
     Step 1
KATO-KATZ TECHNIQUE CONT’D




                Step 3
KATO-KATZ TECHNIQUE CONT’D




     Step 4                  Step 5
KATO-KATZ TECHNIQUE CONT’D




               Step 6
KATO-KATZ TECHNIQUE CONT’D




              Step 7
KATO-KATZ TECHNIQUE CONT’D
CONCENTRATION TECHNIQUES




Flotation                         Sedimentation
Techniques                        Techniques
       Acid Ether Concentration       Zinc Sulfate FT
       Technique
                                      Sheather’s Sugar
      Formalin-Ether CT               FT
                                      Brine Flotation T
FORMALIN- ETHER CONCENTRATION TECHNIQUE
(FECT)



                          Ethyl
                          Acetate



                         Plug of debris



                          Formalin


                         Sediment
ACID ETHER CONCENTRATION TECHNIQUE
(AECT)



                          Ethyl
                          Acetate



                         Plug of debris



                          HCl


                         Sediment
ZINC SULFATE FLOTATION TECHNIQUE

                       Floating Material




                          Zinc Sulfate Sol’n
HARADA MORI CULTURE

                      Culture Tube



                        Strip of filter paper




                      Smear of Feces




                       water

More Related Content

What's hot

Classification of cestodes
Classification of cestodesClassification of cestodes
Classification of cestodesShilpa k
 
Schistosoma ppt dr somesh 2015 - Parasitology - Trematodes
Schistosoma ppt dr somesh 2015 - Parasitology - TrematodesSchistosoma ppt dr somesh 2015 - Parasitology - Trematodes
Schistosoma ppt dr somesh 2015 - Parasitology - TrematodesSOMESHWARAN R
 
Schistosomes
SchistosomesSchistosomes
Schistosomesraj kumar
 
Ancylostoma duodenale (hookwormi)2015
Ancylostoma duodenale (hookwormi)2015Ancylostoma duodenale (hookwormi)2015
Ancylostoma duodenale (hookwormi)2015RaNa MB
 
Taenia solium pork tapeworm
Taenia solium pork tapewormTaenia solium pork tapeworm
Taenia solium pork tapewormAparna Venkat
 
Trichinella Spiralis
Trichinella SpiralisTrichinella Spiralis
Trichinella SpiralisAhmed Essam
 
Intestinal trematodes
Intestinal trematodesIntestinal trematodes
Intestinal trematodesBruno Mmassy
 
Strongyloides stercoralis
Strongyloides stercoralisStrongyloides stercoralis
Strongyloides stercoralismohamed fathy
 
Balantidium Coli
Balantidium ColiBalantidium Coli
Balantidium ColiOsama Zahid
 
Filarial worms dr. rs 28 08-2017
Filarial worms dr. rs 28 08-2017Filarial worms dr. rs 28 08-2017
Filarial worms dr. rs 28 08-2017SOMESHWARAN R
 

What's hot (20)

Classification of cestodes
Classification of cestodesClassification of cestodes
Classification of cestodes
 
Ancylostoma duodenale
Ancylostoma duodenaleAncylostoma duodenale
Ancylostoma duodenale
 
Taenia saginata
Taenia  saginataTaenia  saginata
Taenia saginata
 
Schistosoma ppt dr somesh 2015 - Parasitology - Trematodes
Schistosoma ppt dr somesh 2015 - Parasitology - TrematodesSchistosoma ppt dr somesh 2015 - Parasitology - Trematodes
Schistosoma ppt dr somesh 2015 - Parasitology - Trematodes
 
Schistosomes
SchistosomesSchistosomes
Schistosomes
 
Trichinella spiralis
Trichinella spiralisTrichinella spiralis
Trichinella spiralis
 
Fasciolopsis buski
Fasciolopsis buskiFasciolopsis buski
Fasciolopsis buski
 
Ancylostoma duodenale (hookwormi)2015
Ancylostoma duodenale (hookwormi)2015Ancylostoma duodenale (hookwormi)2015
Ancylostoma duodenale (hookwormi)2015
 
Trichinella spiralis
Trichinella  spiralisTrichinella  spiralis
Trichinella spiralis
 
Taenia solium pork tapeworm
Taenia solium pork tapewormTaenia solium pork tapeworm
Taenia solium pork tapeworm
 
Balantidium coli
Balantidium coliBalantidium coli
Balantidium coli
 
Trichinella Spiralis
Trichinella SpiralisTrichinella Spiralis
Trichinella Spiralis
 
F.buski
F.buskiF.buski
F.buski
 
Taenia solium
Taenia soliumTaenia solium
Taenia solium
 
Intestinal trematodes
Intestinal trematodesIntestinal trematodes
Intestinal trematodes
 
Nematode
NematodeNematode
Nematode
 
Trematodes
TrematodesTrematodes
Trematodes
 
Strongyloides stercoralis
Strongyloides stercoralisStrongyloides stercoralis
Strongyloides stercoralis
 
Balantidium Coli
Balantidium ColiBalantidium Coli
Balantidium Coli
 
Filarial worms dr. rs 28 08-2017
Filarial worms dr. rs 28 08-2017Filarial worms dr. rs 28 08-2017
Filarial worms dr. rs 28 08-2017
 

Viewers also liked

Viewers also liked (20)

Nematodes
NematodesNematodes
Nematodes
 
Bezmugurkaulnieki 1
Bezmugurkaulnieki 1Bezmugurkaulnieki 1
Bezmugurkaulnieki 1
 
Bezmugurkaulnieki 2
Bezmugurkaulnieki 2Bezmugurkaulnieki 2
Bezmugurkaulnieki 2
 
Ascaris.ppt
Ascaris.pptAscaris.ppt
Ascaris.ppt
 
Nematodos
Nematodos Nematodos
Nematodos
 
Primary and Secondary Lymphoid Organs
Primary and Secondary Lymphoid OrgansPrimary and Secondary Lymphoid Organs
Primary and Secondary Lymphoid Organs
 
Lymphoid organs ppt
Lymphoid organs  pptLymphoid organs  ppt
Lymphoid organs ppt
 
Nematodos
NematodosNematodos
Nematodos
 
Phylum nematoda
Phylum nematodaPhylum nematoda
Phylum nematoda
 
Phylum Nematoda
Phylum NematodaPhylum Nematoda
Phylum Nematoda
 
Flagella
FlagellaFlagella
Flagella
 
Roundworms (ascaris)
Roundworms (ascaris)Roundworms (ascaris)
Roundworms (ascaris)
 
Round worms
Round wormsRound worms
Round worms
 
Giardia lamblia
Giardia lamblia Giardia lamblia
Giardia lamblia
 
Malaria(Plasmodium falciparum)- Epidemiology, Life Cycle, Prevention and Erad...
Malaria(Plasmodium falciparum)- Epidemiology, Life Cycle, Prevention and Erad...Malaria(Plasmodium falciparum)- Epidemiology, Life Cycle, Prevention and Erad...
Malaria(Plasmodium falciparum)- Epidemiology, Life Cycle, Prevention and Erad...
 
Plasmodium malarial parasite
Plasmodium   malarial parasitePlasmodium   malarial parasite
Plasmodium malarial parasite
 
Plasmodium falciparum
Plasmodium falciparumPlasmodium falciparum
Plasmodium falciparum
 
flagella structure and function
flagella structure and functionflagella structure and function
flagella structure and function
 
Lecture nematodes
Lecture   nematodesLecture   nematodes
Lecture nematodes
 
Entamoeba histolytica
Entamoeba histolyticaEntamoeba histolytica
Entamoeba histolytica
 

Similar to Nematodes (20)

Filariasis
FilariasisFilariasis
Filariasis
 
The protozoans
The protozoansThe protozoans
The protozoans
 
Amoebiasis by dr najeeb
Amoebiasis by dr najeebAmoebiasis by dr najeeb
Amoebiasis by dr najeeb
 
Intestinal nematodes
Intestinal nematodesIntestinal nematodes
Intestinal nematodes
 
2_5465670154190857740-Copy.pdf
2_5465670154190857740-Copy.pdf2_5465670154190857740-Copy.pdf
2_5465670154190857740-Copy.pdf
 
Rhinosporidiosis
RhinosporidiosisRhinosporidiosis
Rhinosporidiosis
 
Copmlete kingdom animalia topic
Copmlete kingdom animalia topicCopmlete kingdom animalia topic
Copmlete kingdom animalia topic
 
Helminthes
HelminthesHelminthes
Helminthes
 
Leishmania
LeishmaniaLeishmania
Leishmania
 
Other Filarial Worms
Other Filarial WormsOther Filarial Worms
Other Filarial Worms
 
Parasitology-Lab.pdf
Parasitology-Lab.pdfParasitology-Lab.pdf
Parasitology-Lab.pdf
 
7 anthelmintic drugs
7   anthelmintic drugs7   anthelmintic drugs
7 anthelmintic drugs
 
Nematode
Nematode Nematode
Nematode
 
Introduction to Tissue Nematodes and Filarial Worms
Introduction to Tissue Nematodes and Filarial WormsIntroduction to Tissue Nematodes and Filarial Worms
Introduction to Tissue Nematodes and Filarial Worms
 
Introduction to Protozoan parasites
Introduction to Protozoan parasitesIntroduction to Protozoan parasites
Introduction to Protozoan parasites
 
medical-parasitology-power-point-lab8 part2
medical-parasitology-power-point-lab8 part2medical-parasitology-power-point-lab8 part2
medical-parasitology-power-point-lab8 part2
 
Intestinal Nematodes
Intestinal NematodesIntestinal Nematodes
Intestinal Nematodes
 
Introduction to nematodes
Introduction to nematodesIntroduction to nematodes
Introduction to nematodes
 
Cestodes and trematodes
Cestodes and trematodesCestodes and trematodes
Cestodes and trematodes
 
Helminths.pdf
Helminths.pdfHelminths.pdf
Helminths.pdf
 

More from Angelica Nhoj Gemora

More from Angelica Nhoj Gemora (6)

Electrochemistry, electrophoresis, ise
Electrochemistry, electrophoresis, iseElectrochemistry, electrophoresis, ise
Electrochemistry, electrophoresis, ise
 
Community profiling and analysis copy
Community profiling and analysis   copyCommunity profiling and analysis   copy
Community profiling and analysis copy
 
Qsgender09q4
Qsgender09q4Qsgender09q4
Qsgender09q4
 
Qsgender09q4
Qsgender09q4Qsgender09q4
Qsgender09q4
 
Qsgender09q4
Qsgender09q4Qsgender09q4
Qsgender09q4
 
Blood collection
Blood collectionBlood collection
Blood collection
 

Nematodes

  • 2. GENERAL CHARACTERISTICS OF NEMATODES They assume three basic morphologic forms: Egg, Larvae and Adult worms Reproduce sexually with male and female worms Complete digestive tract and complete reproductive systems
  • 3. TAXONOMIC CLASSIFICATION Intestinal Species E. vermicularis T. trichura N. americanus A. duodenale Phylum A. lumbricoides Class S. stercoralis Nemathelminthes Nematoda Intestinal- Tissue Species T. spiralis D. medinensis
  • 4. ASCARIS LUMBRICOIDES Ascaris lumbricoides Common Name Giant Intestinal Roundworm Infective Stage Embryonated Egg Habitat Small Intestine Mode of Transmission Ingestion of contaminated food/ water Diagnostic Specimen Feces
  • 5. ASCARIS LUMBRICOIDES 2 A. lumbricoides Adult worms
  • 6. ENTEROBIUS VERMICULARIS Enterobius vermicularis Common Name Pinworm Infective Stage Embryonated Egg Habitat Large Intestine Mode of Transmission Ingestion of egg/ autoinfection Diagnostic Specimen Feces/ Cellophane Tape prep
  • 7. TRICHURIS TRICHURA Trichuris trichura Common Name Whipworm Infective Stage Embryonated Egg Habitat Large Intestine Mode of Transmission Ingestion of egg via contaminated food/ water Diagnostic Specimen Feces
  • 9. NECATOR AMERICANUS AND ANCYLOSTOMA DUODENALE Necator americanus and Ancylostoma duodenale Common Name Hookworm Infective Stage Third Stage Filariform Larvae Habitat Small Intestine Mode of Transmission Skin Penetration of Infective Larvae Diagnostic Specimen Feces
  • 10. HOOKWORMS 2 A. duodenale N. americanus
  • 11. STRONGYLOIDES STERCORALIS Strongyloides stercoralis Common Name Threadworm Infective Stage Third Stage Filariform Larvae Habitat duodenum and upper jejunum Mode of Transmission Skin Penetration of Infective Larvae/ autoinfection Diagnostic Specimen Feces
  • 12. TRICHINELLA SPIRALIS Trichinella spiralis Common Name Trichinia worm Infective Stage Encysted Larvae Habitat Striated muscle tissue Mode of Transmission Ingestion of raw/ undercooked contaminated meat Diagnostic Specimen skeletal muscle biopsy/ Blood (LDH, Aldolase, CPK, eo ct)
  • 13. CAPILLARIA PHILIPPINENSIS Capillaria philippinensis Common Name NA Infective Stage Encysted Larvae Habitat Small Intestine Mode of Transmission Ingestion of raw/ undercooked contaminated fish Diagnostic Specimen Feces
  • 14. THE FILARIAE- GENERAL CHARACTERISTICS Two morphologic forms: Adult worms and Microfilariae Adult worms are usually creamy white and assume a filariform shape Have both male and female forms Females lay live microfliariae Require arthropod vectors Exhibit periodicity (Nocturnal/ diurnal)
  • 15. WUCHERERIA BANCROFTI Wuchereria bancrofti Common Name Bancroft's Filaria Infective Stage L3 Larva Habitat Lymphatics and blood Mode of Transmission Bite from infected mosquito (Culex, Aedes or Anopheles) Diagnostic Specimen Giemsa stained smear (collected at night)/ Knott's Technique
  • 16. BRUGIA MALAYI Brugia malayi Common Name Malayan Filaria Infective Stage L3 Larva Habitat Lymphatics and Blood Bite from infected mosquito ( Anopheles, Aedes, Mansonia, Mode of Transmission Amigeres) Diagnostic Specimen Giemsa stained smear (collected at night)/ Knott's Technique
  • 17. WUCHERERIA BANCROFTI V.S. BRUGIA MALAYI Distinguishing Wuchereria Brugia malayi Characteristics bancrofti Sheath in Giemsa unstained Pink Nuclei Regularly spaced, Irregularly spaced separately situated and overlapping Tail none Single row of nuclei that reach tail’s end Terminal Nuclei None 2 nuclei, which bulge the cuticle; conspicuously placed Appearance in Blood Smoothly curved Kinky film
  • 18. LOA LOA Loa loa Common Name African Eye Worm Infective Stage L3 Larva Habitat Subcutaneous tissue, the eye and bridge of nose Mode of Transmission Bite from infected Chrysops silacea or C. dimidiata (Deer Fly) Diagnostic Giemsa stained smear (collected at midday 10:15to 2:15)/ Knott's Specimen Technique
  • 19. ONCHOCERCA VOLVULUS Onchocerca volvulus Common Name Blinding Filaria Infective Stage L3 Larva Habitat Subcutaneous tissue, nodules and eyes Mode of Transmission Bite from infected Simulium Blackfly Diagnostic Specimen Giemsa stained smear of skin snips
  • 20. MANSONELLA OZZARDI AND MANSONELLA PERSTANS Mansonella ozzardi and Mansonella perstans Common Name Ozzard's Filaria and Perstans Filaria Infective Stage L3 Larva Habitat Blood and various tissue Mode of Transmission Bite from infected Simulium Blackfly or Culicoides (sucking midge fly) Diagnostic Specimen Fresh Giemsa stained blood smear
  • 21. Long Quiz next meeting?  The End
  • 22. PART 2: KATO-KATZ, KATO-THICK AND CONCENTRATION TECHNIQUES
  • 23. KATO KATZ TECHNIQUE Step 2 Step 1
  • 29. CONCENTRATION TECHNIQUES Flotation Sedimentation Techniques Techniques Acid Ether Concentration Zinc Sulfate FT Technique Sheather’s Sugar Formalin-Ether CT FT Brine Flotation T
  • 30. FORMALIN- ETHER CONCENTRATION TECHNIQUE (FECT) Ethyl Acetate Plug of debris Formalin Sediment
  • 31. ACID ETHER CONCENTRATION TECHNIQUE (AECT) Ethyl Acetate Plug of debris HCl Sediment
  • 32. ZINC SULFATE FLOTATION TECHNIQUE Floating Material Zinc Sulfate Sol’n
  • 33. HARADA MORI CULTURE Culture Tube Strip of filter paper Smear of Feces water