Amoeba: Pseudopodia spp. Entamoeba histolytica, Entamoeba coli, Iodamoeba butschlii, Dientamoeba fragilis Philippine Medically Important Protozoan species

1. Notes on Philippine Medically Important Protozoans Part 1 (Amoeba)
Meccar Moniem Elino, BSBio IV , Davao Doctors College
Instructor: Mrs. Chalee Sienes-Reyes, RMT, MSMT, Ph.D
Reference: Philippine textbook of Medical Parasitology 2nd ed.(2004) UP. Edited by Vicente Y. Belizario, Jr. and Winifreda U. de Leon
Cyst Tropohozoite
No. of Chromatoidal Chromatoidal
Size/Shape Cystic Wall Cytoplasm Karyosome Size/Shape Cytoplasm Endoplasm Nucleus karyosome
Nucleus Bars Bars
nucleus
membrane is
rounded grayish vacuoles
single, lined by fine,
smooth, ends with 10- violet with bluish black
Entamoeba histolytica 5-20micra/spherical 1 to 4 central spherical regularly small, center
definite glycogen 60micra/irr clear with
nucleus; arranged
mass demarcation ingested rbc
chromatin
granules
15-50micra, spherical with
splintered grayish highly
condensed, thick nuclear irregular
smooth, ends with violet; there vacuolated
Entamoeba coli 10-33micra/spherical 1 to 8 eccentric thickened, membrane chromatin large eccentric
definite glycogen is no clear containing
rounded lined with granules
mass demarcation bacteria
mass coarse
1 to 4 deeply vacuole may
smooth, grayish 6- grayish small/spherical large irregular
Endolimax nana 6-10micra/oval darkly stained contain
definite granular 15micra/irr violet largenucleus centric/eccentric
stained curved rods bacteria
with clear
demarcation
of ecto and with
5-
Entamoeba gingivalis x x x x x x endo, same bacteria and spherical center
35micra/irr
pseudopodia rare rbc,
direction of
E. histolytica
violet-gray
vacuoles
granular absent or
smooth, 6- granular, may contain single large
Iodamoeba butschlii 6 – 15 micra/irr 1 or rarely2 with large with stained
definite 25micra/irr vacuolated bacteria and /spherical central/eccentric
vacuole small rods
yeast cells
unsained
granular,
with 4-8 with 4-8
3- vacuolated
Dientamoeba fragilis x x x x x x chromatin 2 chromatin
22micra/irr with
granules granules
bacteria