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L24
1. Lecture 24 Differentiation and stem cells *Stem cells and differentiation in plants Totipotency Stem cells in animals Therapeutic use Cloning Therapeutic Reproductive Therapeutic cloning in humans
2. Stem cells Stem cells - undifferentiated cells that divide and give rise to cells that differentiate into specialized cells of plant and animal tissues Self-renewal Differentiation ECB 21-35
4. Cell fate in root is determined by position Meristem renewal Differentiation Cells leave meristem and enter files (colors) and differentiate into specific fates (stele, endodermis, cortex etc.) endodermis cortex stele
5. Cells of adult plants remain totipotent: cloning a carrot Moore et al Figure 9.2 Wm C Brown Publishing 1 mm 3 fragments (“explants”) from adult root… Culture explants in liquid culture medium… Cells “dedifferentiate” and begin to divide, forming “callus” tissue… Induce with hormones to initiate shoot and root formation… Culture “embroid” in liquid culture, then agar… Move to soil… Regenerated adult plant…
6. Cells from young animal embryos are also totipotent Totipotent - capable of forming all differentiated cells of adult Pluripotent - capable of forming more than 1 differentiated cell type ECB 21-40 Embryonic stem cells (ES cells)
10. Differences in gene expression make all cell types of organism unique ECB 8-15 Genes A, B , C, D smooth muscle transcribes A, B hepatocytes A, C Lymphocytes B, C, D 35,000 -40,000 genes allow nearly infinite combinations to define cell type
11. Stem cells that resupply differentiated cells are pluripotent: example blood Hemopoetic stem cell: Divides to renew itself for lifespan of animal Can form a limited number of cell types (pleuripotent) But not differentiated Blood cells must be renewed but not capable of cell division (red blood cells lack a nucleus) ECB 21-39
13. Lecture 24 Differentiation and stem cells Stem cells and differentiation in plants Totipotency Stem cells in animals Therapeutic use Cloning Therapeutic Reproductive Therapeutic cloning in humans
14. Stem Cells -- therapeutic use? • Embryonic stem cells donated embryos from In Vitro Fertilization clinics • 4-5 days old (blastocyst stage) • cultured cells grow in petri plates (30 cells --> millions after ~6 months • Conduct research to try to induce them to differentiate into specialized cell type of interest • Great potential for therapeutic uses: -inject patient with stem cells that are induced to differentiate into defective cell, tissue
15. Parkinson’s disease Loss of dopamine-producing cells in the brain Goal: stem cell replacement Using embryonic stem cells from patient would eliminate risk of rejection Hope for treatment of diabetes, osteoarthritis etc. Mouse embryonic stem cells -- cured mouse Parkinson’s disease (model system)
16. Federal Regulations G.W. Bush: August 2001: federally-funded research - can only use previously isolated ES cells (~17 lines in use, most in private laboratories) 2 issues with ES cells: 1. The source 2. The potential to clone humans
17. Two types of cloning: reproductive and therapeutic ECB 21-41 Reproductive cloning has been accomplished for large mammals, not humans Therpeutic cloning in humans reported two months ago Somatic nucleus must be reprogrammed to embryonic program by egg cytoplasm Somatic cell nuclear transplant (SCNT)
18. Reproductive cloning of Dolly the sheep Q: other animal species cloned? A: Mice, pigs, cats, cows, mule, horse etc
19. Banteng: endangered cow species San Diego Zoo: frozen tissue Used dolly-type cloning, frozen nucleus implanted into a regular cow cell
21. Rhesus Monkey model for primate cloning, no success! Problems in mitosis following nuclear transplant Tripolar spindle Regular fertilized egg. Green = centrosome protein In primates, removal of nucleus also removes most of the spindle proteins. Aberrant cell division--> gross chromosomal segregation defects.
22. Existing ES lines created by in vitro fertilization In vitro fertilization (IVF): use normal human egg/sperm for fertilization followed by lab culture until young embryo and then implant into female Rather than implant, these embryos can be used to isolate ES cells About half of embryos made by IVF yield ES cell lines But no success with nuclear transplant method until recently…….. Hwang et al., Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst. ScienceExpress 12 Feb 2004 Go to Marriot library, and log onto http://www.sciencemag.org/cgi/rapidpdf/1094515v1
23. Experimental procedure for therapeutic human cloning ECB 21-41 Somatic cell nuclear transplant (SCNT) Cumulus cells from ovary (2N) Poke hole in eggs and gently extrude spindle No needles! Electrofusion of cells 242 eggs from 16 women: Voluntary donors 20 blastocyst embryos 1 ES line (much lower than 50% of blastocysts using IVF)
24. Images of enucleation and ES colonies Spindles before enucleation After: spindles outside egg Light microscopy of human ES cell colonies Immunofluorescence for nestin (marker of ES cells) Karyotype (2N)
25. Human ES cells cause teratomas in immunodeficient mice Teratoma = cancerous tissue containing lots of different cell types glandular epithelium with smooth muscle and connective tissue Neuroepithelial rosset pigmented retinal epithelium ostoid island showing bony differentiation cartilage Shows pleuripotency of human ES line