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Evolution of DNA Sequencing - talk by Jonathan Eisen for the Bodega Workshop in Applied Phylogenetics
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Talk by Jonathan Eisen on the Evolution of DNA Sequencing Methods
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Evolution of DNA Sequencing by Jonathan Eisen (for UC Davis Workshop in Applied Phylogenetics at Bodega Bay Marine Lab)
Evolution of DNA Sequencing by Jonathan Eisen
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Next generation Sequencing or massive parallel sequencing is a high throughput approach to sequence genetic material using the concept of massively parallel processing. It is also called second generation sequencing.This enables researchers a wide variety of applications & study biological systems.
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these slides analyze the rapid improvements in DNA sequencers and the implications for these rapid improvements for drug discovery, new crops, materials creation, and new bio-fuels. Many of the rapid improvements are from "reductions in scale." As with integrated circuits, reducing the size of features on DNA sequencers has enabled many orders of magnitude improvements in them. Unlike integrated circuits, the improvements are also due to changes in technology. For example, changes from pyrosequencing to semiconductor and nanopore sequencing have also been needed to achieve the reductions in scale. Second, pyrosequencing also benefited from improvements in lasers and camera chips.
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Evolution of DNA Sequencing by Jonathan Eisen (for UC Davis Workshop in Applied Phylogenetics at Bodega Bay Marine Lab)
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Jonathan Eisen
Next generation Sequencing or massive parallel sequencing is a high throughput approach to sequence genetic material using the concept of massively parallel processing. It is also called second generation sequencing.This enables researchers a wide variety of applications & study biological systems.
Next Generation Sequencing
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Amritha S R
these slides analyze the rapid improvements in DNA sequencers and the implications for these rapid improvements for drug discovery, new crops, materials creation, and new bio-fuels. Many of the rapid improvements are from "reductions in scale." As with integrated circuits, reducing the size of features on DNA sequencers has enabled many orders of magnitude improvements in them. Unlike integrated circuits, the improvements are also due to changes in technology. For example, changes from pyrosequencing to semiconductor and nanopore sequencing have also been needed to achieve the reductions in scale. Second, pyrosequencing also benefited from improvements in lasers and camera chips.
DNA sequencing: rapid improvements and their implications
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Jeffrey Funk
ASLO 2017 Aquatic Sciences Meeting: Mountains to the Sea; Feb 26 - Mar 3; Honolulu, Hawai’i, USA; Invited talk
A decade into Next Generation Sequencing on marine non-model organisms: curre...
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SANGER SEQUENCING - 1st generation sequencing Sanger Sequencing Workflow: PCR amplification (target enrichment) PCR purification (primer, dNTPs) Sequencing reaction (bi-directional) Sequencing purification (primer, dNTPs, ddNTPs) Electrophoretic run on sequencer Sequencing lecture Alignment to reference SANGER SEQUENCING: LIMITATIONS Analytical sensitivity*: 99% PCR-Based no detection deletion/duplication rearrangements del/dup BRCA = 4-28% of all BRCA mutations in most population** Level of mosaicism > 20% Low throughput (82496 capillary tubes) Labor intensive Time consuming High cost (large size gene or more genes)
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