1. A Pictorial representation of what I am currently doing:
MTT assays.
Fig 1: Orange color due to addition of MTT reagent to detect cell viability in each
well
Fig 2: Initial plating and treatment of mammalian cells in 10% FBS media

2. FACS analysis performed with 6 well plates.
Cells per well : 175K
Media: 3 mL in each well
2-Day to 3- Day treatments depending on the purpose
ROS determining agent added on Day 2 of the treatment, prepared fresh.
Also done to determine Annexin-PI binding of cells showing usual apoptosis
(e.g Annexin binds to the phospholipids, facing outside, of a programmed to die
cell and thus this activity can determine apoptosis/necrosis of the treated cells)

3. DRUG PREPARATIONS (High school Chemistry )
DRUGS kept on ice before use
DRUG samples (including stocks) in Storage
FRESH drug dilutions prepared for treatment