14. PBS
Chemicals used in PBS: PBS and H2O or D2O
• Dissolve 1 table in
200mL of H2O or D2O
15. BGB
Blotting Grade Blocker
To make 5mg/ml BGB
solution in popping
buffer:
•First wiight out 15mg of
BGB poweder
•Add 1.5 ml of 1x pop
and mix by vortexing
•Run through .2μM
syringe filter using 3ml
syringe.
•Now store 5mg/ml BGB
in 1x pop buffer (minus
amount of protein that
stuck to filter) at 3C.
This should be good for
few weeks.