Fiber-light i invented for Olympus microscope for Optical Tweezers.

1. Fiber-light for Olympus Microscope
Optical Tweezers
Fiber-light system for
Olympus IX71 microscope
How to make a fiber light system for your microscope?

2. Optical Tweezers enclosure
Microscope wall

3. Microscope wall temperature inside enclosure compared: Halogen lamp Vs Fiber-light

4. Why fiber-light is important ?
• DNA experiments are really sensitive to temperature (thermal
energy) even few degrees make a big difference.
• Microscope (optical trap) is enclosed inside an enclosure (to avoid
airborne noise and currents), so within few hours temperature of the
microscope wall rises almost twice due to the halogen lamp used for
illumination, this heats up the entire enclosure so as the sample.
• Temperature hike may make two similar data sets which are taken
some time apart different.
• Fiber light solves the problem of temperature hike by providing clean
light and by removing heat production through the lamp and
conduction through the microscope skin.
• With fiber light temperature inside the enclosure goes from 23.7 to
25.5 total 1.8 degree Celsius hike in comparison to halogen lamp
where temperature goes from 23.7 to 49 total 25.3 degree hike over a
period of 10 hours (see slide 3).

5. Construction of Fiber-light system
Fiber light has 4 parts
Olympus power
supply Model
TH4-100
Dolan Jenner
optical fiber bundle
Fiber-light feeder
Illuminator & fiber
plugin adapter

6. Olympus Power supply
Model TH4-100
TH4-100 has 4 holes
Connect +ve 12V and
ground with a wire
at one end and other
end is banana
connectors

7. Construction of fiber plugin adapter at
illuminator
Illuminator
Fiber plugin
adapter

8. Construction Steps
Remove illuminator from microscope
Remove the Halogen lamp Remove all the enclosures

9. Assemble the fiber plugin
ER2-thorlsba 2’’ cage assembly rod
AD11F-thorlabs adapter
S1TM08-thorlabs lens cell adapter
SM1L03-thorlabs lens tube
CP02-thorlabs cage plate

10. Put lens cell adapter on the cage plate Put lens tube on different cage plate and assemble
Put other lens cell on one end of cage plate and adapter on other end

11. Fixing the Illuminator
center
wall
Little stage
Make 2 notches aligned with center of holes on the cage

12. Make a through hole ¾ cm from the notch and from the edge

13. Make a metal piece looks like in the picture the hole are aligned with each other

14. Put the piece such the hole are aligned

15. Put the plugin on the illuminator
Put the screws

16. Put a screw and a nut

17. Illuminator with fiber plugin adapter is complete

18. Cushion
Z- adjustment
Screws
Put a rubber cushion (optional) and insert the fiber through the opening and tight the screws

19. Construction of Fiber-feeder
Lamp mount
Lens assembly Fiber outlet adapter
6 X3X2.5 inches aluminum box

20. Banana connectors Prepare the box
3/8’’ hole for 3/8’’ holes for rubber legs
lamp mount
1.25’’
2.25’’ .5’’
.5’’
50mm
.25’’
Lamp side
Bottom side
¼’’ hole for lens assembly
3/8’’ hole for fiber outlet adapter
1’’ hole for fiber outlet
Fiber outlet side

21. Preparing Lamp Mount
PCB plates
Banana connectors
Metal plates
Halogen lamp
4/40’’ blind hole ½’’ long in PCB
WARNING: Lamp gets really HOT so DON’T use plastic, wood or anything which burns below 500o Celsius

22. 3/8’’ through holes
• I used 6 fold of PCB plates to
mount the metal plates with ½’’
long blind screws.
• Metal plates are aluminum plates
which I machined to fit the lamp
wire connectors.
• Metal plates are attached into
PCB plates with 4/40’’ blind screws.
• PCB plates are screwed to the wall
with 3/8’’ screws.
• PCB plate can tolerate high
temperatures and provide
good isolation for the metal plates.
4/40’’ screw on 3/8’’ holes
in metal

23. Preparing Fiber outlet adapter
CP02T-thorlabs cage plate
SM1T2-Thorlabs coupler SM1A1-Thorlabs adapter

24. Insert adapter into coupler and then
coupler into cage plate
• On wood side cage plate do not use
the wood like shown in the picture.
• Put another cage plate on that side
with another adapter and coupler.
• I did not have extra set in my lab
so I used wood.

25. Mount in with 3/8’’ screw to the box
Do not use wood use 2 cage plates with 2 couplers and 2 adapters

26. Preparing Lens assembly
CP02T-thorlabs cage plates
LA1951-A thorlabs f25mm lens
RA90-thorlabs post clamp
ER2-thorlsba 3’’ cage assembly rod
TR2-thorlabs post
Lens is to focus the light on the fiber inlet

27. Put lens assembly on the box
Slide in z-direction to get optimum spot on the fiber inlet
50mm
xy-adjustment to center the spot (lamp image) on the fiber inlet
Lens assembly gives 3-D adjustment to the lens

28. Bad alignment
Good alignment

29. Spot can be further optimized by looking into illuminator

30. Illuminator at low intensity

31. Prepare the cover
• Halogen lamp heats up the box
• I did not put any fan inside because
fan generates vibrations which can
travel through the fiber bundle to
the microscope and make optical
trap noisy.
• So fan has to be outside with no
physical contact with the box.
• To accommodate that I made 2’’
diameter holes.
• Fan will be mounted from top
trough the roof.

32. Mounted Fan

33. Bundle is now complete

34. Mount the illuminator on the microscope

35. Fiber-light with Optical Tweezers