1. Gene transcription is coordinated with, but not
dependent on, cell divisions during C. elegans
embryonic fate specification!
Gautham
Nair,
Travis
Walton,
John
Isaac
Murray,
and
Arjun
Raj
University
of
Pennsylvania
Department
of
Bioengineering
University
of
Pennsylvania
Department
of
GeneDcs

2. RNA-FISH!
10µm!
RNA!

3. Automated
Lineaging!

4. Gut genes!
Wild type
20C!

7. Transcription
is slowed
LESS?

8. Transcription
is slowed
MORE?

9. Transcription
is slowed
THE SAME?

10. Transcription
is slowed
THE SAME

11. Transcription
is slowed
THE SAME
- many other genes
- clk-1 mutant slowdown

12. Transcription
is slowed
THE SAME
- many other genes
- clk-1 mutant slowdown

13. Transcription
is slowed
THE SAME
Total Number of Cells in Embryo

14. Total Number of Cells in Embryo

15. Total Number of Cells in Embryo
Transcription is
DEPENDENT
on cell divisions?

16. emb-29(g52)
mutant
Total Number of Cells in Embryo
Transcription is
DEPENDENT
on cell divisions?
NO

17. Transcription is
DEPENDENT
on cell divisions?
NO
- elt-2 in E lineage
- hlh-1 and
unc-120
in muscle
pathway

18. Transcription is
DEPENDENT
on cell divisions?
NO
- elt-2 in E lineage
- hlh-1 and
unc-120
in muscle
pathway

19. Transcription is
DEPENDENT
on cell divisions?
NO
- elt-2 in E lineage
- hlh-1 and
unc-120
in muscle
pathway

20. Gene transcription is coordinated with, but not
dependent on, cell divisions during C. elegans
embryonic fate specification!

21. Gene transcription is coordinated with, but not
dependent on, cell divisions during C. elegans
embryonic fate specification!
Funding:
HHMI
Life
Sciences
Research
FoundaDon
Fellowship
Burroughs-­‐Wellcome
Fund
NIH
New
Innovator
Award
Penn
Genome
FronDers
InsDtute
Pennsylvania
Department
of
Health