The following is a presentation to supplement a genetics class. The intention is to help build interest by showing dog breeding and how the scientific method has uncovered remarkable information about the morphological changes in dog breeds.

1. What can your Dog teach you about Molecular Genetics?

3. Why have dogs become very interesting to molecular geneticists?

7. Quick Review of Genetic Terms and Principles Review of Genetics Terms and Principles

8. Review - Big Ideas in Genetics One gene – One phenotypic trait Recessive genes can hide under Dominant Dominant phenotype One phenotype – different genotypes! or BB Bb

9. Review - Big Ideas in Genetics * http://en.wikipedia.org/wiki/Qtl QTL – Quantitative Trait Loci 1908 Nilson-Ehle Many genes + environment One quantitative trait =

10. Review - Big Ideas in Genetics Single Nucleotide Polymorphism * http://en.wikipedia.org/wiki/Qtl Animation http://www.dnai.org/text/mediashowcase/index2.html?id=1083

11. only P1 and P4 have the DNA from grandparents G1 and G4 and thus, it is possible that the disease gene might be somewhere near ? . ~85% probability of finding a simple recessive trait. Review - Gene Markers for Beginners Now imagine that grandparents G1 and G4 and puppies P1 and P4 have the disease and we're looking at a recessive mode of inheritance. We would then look at the DNA markers we have and see that at position ?

13. Founders Effect Original Population Survivors are selected for breeding New Population

14. Population BottleNeck

15. Bottlenecked Gene Pool Wolf Dog AKC Breeds Wade “The Dog Genome:Sequence, Evolution, and Haplotype Structure” Broad Institute MIT

18. Documented PWD breeding lines Lark Web Site: Genetics of quantitative traits in Portuguese Water Dogs http://64.226.94.9/npwd.htm

19. Who’s your Daddy’s Daddy’s Daddy?

20. With high level of inbreeding all dog would look the same? Polymorphic

21. Genetic Resource Lark Web Site: Genetics of quantitative traits in Portuguese Water Dogs http://64.226.94.9/npwd.htm

22. Zero Correlation Pedigree Markers

23. Is there a correlation between genetic markers in PWD and breeding lineage? Lark Web Site: Genetics of quantitative traits in Portuguese Water Dogs http://64.226.94.9/npwd.htm

24. Results - QTL linking the skull, legs ,hip Courtesy Sanger Inst http://www.georgieproject.com/x-ray/x-ray.htm

25. Results - QTL Size IGF1 Chrom 15 http:// www.ncbi.nlm.nih.gov/mapview/map_search.cgi?taxid =9615&build= current&advsrch = off&query =igf1

26. Elegance of the Georgie experiment Free living population of animals versus a colony of laboratory dogs that are inbred.

27. Georgie and my dog Are there similar DNA sequences in my dog that will make him susceptible to autoimmune disease? Georgie McGyver

28. If you can find a population

29. If you can build a database of BioMarkers

30. Biomarker Research Protein Identification Protein Digest Sample Handling Data Analysis Peptide Separation Data Storage Cells or Tissue Thermo Scientific Laboratory Workflow Sample Preparation Data Interpretation & Storage Sample Analysis Robotics Centrifuges Concentrators Liquid Chromatography/Mass Spectrometry Lab Information Management System Microplate Readers Software Xcalibur Protein Fractionation Freezers Biomarker Identified