![Molecular Genetics [C2]
Unit 5. Molecular Genetics (15 days)
Textbook Readings
Chapters 16, 17, 18, 19, 20
Essential Knowledge
2.E.1 Timing and coordination of specific events are necessary for the normal development of an
organism, and these events are regulated by a variety of mechanisms
3.A.1 DNA, and in some cases RNA, is the primary source of heritable information
3.B.1 Gene regulation results in differential gene expression, leading to cell specialization
3.B.2A variety of intercellular and intracellular signal transmissions mediate gene expression.
3.C.1 Changes in genotype can result in changes in phenotype
3.C.3 Viral replication results in genetic variation, and ciral infection can introduce genetic
variation into the hosts
4.A.3 Interactions between external stimuli and regulated gene expression result in specialization
of cells, tissues, and organs
Laboratory Exercises
AP® Biology Laboratory 6: Molecular Biology [C8]
o Goal: To understand bacterial transformation, prepare competent E.coli cells, to transfer
genes using a plasmid, to cut DNA using restriction endonucleases, to use gel
electrophoresis to separate DNA fragments for later analysis (wet lab)
Or
Recombinant DNA paper lab
Or virtual lab](https://image.slidesharecdn.com/moleculargeneticsunitataglance-140109095112-phpapp01/85/molecular-genetics-unit-at-a-glance-1-320.jpg)
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Molecular Genetics Unit Guide
- 1. Molecular Genetics [C2] Unit 5. Molecular Genetics (15 days) Textbook Readings Chapters 16, 17, 18, 19, 20 Essential Knowledge 2.E.1 Timing and coordination of specific events are necessary for the normal development of an organism, and these events are regulated by a variety of mechanisms 3.A.1 DNA, and in some cases RNA, is the primary source of heritable information 3.B.1 Gene regulation results in differential gene expression, leading to cell specialization 3.B.2A variety of intercellular and intracellular signal transmissions mediate gene expression. 3.C.1 Changes in genotype can result in changes in phenotype 3.C.3 Viral replication results in genetic variation, and ciral infection can introduce genetic variation into the hosts 4.A.3 Interactions between external stimuli and regulated gene expression result in specialization of cells, tissues, and organs Laboratory Exercises AP® Biology Laboratory 6: Molecular Biology [C8] o Goal: To understand bacterial transformation, prepare competent E.coli cells, to transfer genes using a plasmid, to cut DNA using restriction endonucleases, to use gel electrophoresis to separate DNA fragments for later analysis (wet lab) Or Recombinant DNA paper lab Or virtual lab
- 2. Important AP topics per chapter Chapter 16: Molecular Basis of Inheritance Transformation DNA replication Leading vs. lagging strand Telomeres Chapter 17: Gene to Protein Codons Transcription vs. translation (summary only) Introns vs. exons Ribozymes Point mutations Chapter 18: Genetics of Viruses and Bacteria Viral structure Lytic cycle vs. lysogenic cycle Reverse transcriptase Viroid vs. prion Transformation vs. conjugation Transposition Operons (Lac vs. Tryp) Chapter 19: Eukaryotic Genomes Nucleosome Histones Methylation Transcription factors RNA processing Chapter 20: DNA Technology DNA cloning overview Restriction enzymes Vector Probe cDNA Gel electrophoresis RFLPs DNA sequencing Gene therapy DNA fingerprint