These slides are from 3DSIG 2014, presented on July 11.
I describe our investigation of internal symmetry in protein structures. This is quite common (24% of domains), and has many implications for function, folding, and evolution.
I introduce the CE-Symm method, described in
Myers-Turnbull, D., Bliven, S. E., Rose, P. W., Aziz, Z. K., Youkharibache, P., Bourne, P. E., & Prlić, A. (2014). Systematic Detection of Internal Symmetry in Proteins Using CE-Symm. Journal of Molecular Biology, 426(11), 2255–2268. doi:10.1016/j.jmb.2014.03.010
I discuss the results from running CE-Symm across the PDB, as well as some particularly compelling examples.
See also my poster by the same title for more details.
Biopesticide (2).pptx .This slides helps to know the different types of biop...
3DSIG 2014 Presentation: Systematic detection of internal symmetry in proteins
1. Spencer Bliven
July 11, 2014
3DSIG 2014
Myers-Turnbull, D., Bliven, S. E., Rose, P. W., Aziz, Z. K., Youkharibache, P., Bourne, P.
E., & Prlić, A. (2014). Systematic Detection of Internal Symmetry in Proteins Using CE-
Symm. Journal of Molecular Biology, 426(11), 2255–2268. PMID 24681267
6. ! Evolution
! Identify duplications & fusions
! Many examples of homologous quaternary symmetric/
internally symmetric proteins
! Tradeoff between monomer & oligomer
Lee and Blaber. PNAS (2011) vol. 108 (1) pp. 126-30
7. E. Coli DNA polymerase III beta
subunit [1mmi]
! 2 chains (C2 crystal axis)
Human proliferating cell nuclear
antigen [1VYM]
! 3 chains (C3 crystal axis)
8. E. Coli DNA polymerase III beta
subunit [1mmi]
! 2 chains
! 6 domains (pseudo C6)
Human proliferating cell nuclear
antigen [1VYM]
! 3 chains
! 6 domains (pseudo C6)
9. ! 2-3 chains
! 6 domains
! 12 structural repeats (pseudo D6)
Ancient 12-mer?
Ancient 6-mer
Bacterial DimerEukaryotic/Archaeal/
Viral Trimer
Kelman, Z., & O'Donnell, M. (1995). Nucleic Acids Research, 23(18), 3613–3620.
Neuwald, A. F., & Poleksic, A. (2000). Nucleic Acids Research, 28(18), 3570–3580.
10. ! Extends Combinatorial
Extension (CE) algorithm
for structural alignment
! Web server:
source.rcsb.org/
jfatcatserver/symmetry.jsp
! Download & Source code:
github.com/rcsb/symmetry
(LGPL)
Shindyalov, I. N., & Bourne, P. E. (1998). Protein Engineering,
11(9), 739–747.
Jia, Y., Dewey, T. G., Shindyalov, I. N., & Bourne, P. E. (2004).
J Comput Biol, 11(5), 787–799.
11. Fibroblast Growth
Factor [3JUT]
120° 120°
Myers-Turnbull, D., Bliven, S. E., Rose, P. W., Aziz, Z. K., Youkharibache, P., Bourne, P.
E., & Prlić, A. (2014). Journal of Molecular Biology, 426(11), 2255–2268.
12. Fibroblast Growth
Factor [3JUT]
120° 120°
Myers-Turnbull, D., Bliven, S. E., Rose, P. W., Aziz, Z. K., Youkharibache, P., Bourne, P.
E., & Prlić, A. (2014). Journal of Molecular Biology, 426(11), 2255–2268.
15. ! All domains from SCOPe 2.03
! Interactive results:
source.rcsb.org/jfatcatserver/scopResults.jsp
! Underestimate based on conservative thresholds
SCOP Class Superfamilies % Symmetric
α 507 18.5%
β 354 24.6%
α/β 244 16.8%
α+β 551 14.3%
Multi-domain 66 4.5%
Membrane 109 23.8%
Overall 1831 18.0%
16. ! PTS sorbitol transporter subunit IIA
! Novel fold
! Solved by the Protein Structure Initiative
! Structural alignment reveals a conserved sequence
motif between halves
[2F9H]
17. ! 18-24% of domains have internal symmetry
! Symmetry gives clues about duplication events
! Symmetry is deeply tied to protein function
! CE-Symm can accurately detect internal symmetry
d1su3a2 D1pt2a_ d1c5ka1 d1k3ia3 d1h9ya2
18. ! UC San Diego/RCSB
! Douglas Myers-Turnbull
! Andreas Prlić
! Peter Rose
! Zaid Aziz
! Milton Saier
! RCSB & Bourne Lab
members
! NIH
! Philip Bourne
! Philippe Youkharibache
! David Landsman
! Paul Scherrer Institute
! Guido Capitani & Lab
members
Resources:
! source.rcsb.org/jfatcatserver/
symmetry.jsp
! github.com/rcsb/symmetry
! Poster 25
! www.slideshare.net/sbliven
! Funding: NSF, NIH, DOE,
Open Science Grid
19.
20. Glyoxalase I from
Clostridium
acetobutylicum [3HDP]
(Nickel; Dimer)
Glyoxalase I from E.
coli [1F9Z]
(Nickel; Dimer)
1,2-dihydroxy-
naphthalene
dioxygenase from
Pseudomonas sp.
strain C18 [2EHZ]
(Iron; Octamer)
21. Glyoxalase I from
Clostridium
acetobutylicum [3HDP]
(Nickel; Dimer)
Glyoxalase I from E.
coli [1F9Z]
(Nickel; Dimer)
1,2-dihydroxy-
naphthalene
dioxygenase from
Pseudomonas sp.
strain C18 [2EHZ]
(Iron; Octamer)