1. 1.1.3 Cell Division, Cell Diversity and cellular organisation Objectives Cell cycle – stages (Go, S, G1, M); mitosis and meiosis. Budding in yeast; binary fission (bacteria) Significance of mitosis and meiosis; Mitosis – produces clones; growth, asexual reproduction, cancer. Meiosis – gametes; maintain chromosome number; cell produced not genetically identical DNA, genes and packaging information Clones and stem cells (embryonic and adult) Differentiation of stem cells – blood cells (RBC, neutrophils); cambium (xylem + phloem) Cell specialisation - epithelial cells (squamous + ciliated); sperm; palisade; root hair cell; guard cell; neurones Cells, tissues, organs, organ systems HW – Past paper question

2. The Cell Cycle, Mitosis, and Meiosis New cells can only be made when existing cells divide. All cells have the ability to divide – but some cells lose this ability. Intestinal epithelial lining - replaced every five days by cell division Liver cells - divide only to repair damage, and then stop dividing Bone marrow cells - divide repeatedly to produce red and white blood cells Meristem cells (tips of roots and shoots) – divide to produce new growth Cambium cells (plants) – divide to form vascular tissue (xylem and phloem) These are relatively unspecialised cells. Specialised cells often go through the cell cycle only once - the nerve cells, once formed cannot divide again. In eukaryotic cells, there are two types of cell division – mitosis and meiosis . Mitosis is used to produce new cells for growth and repair. Meiosis is used in the formation of gametes only. In prokaryotes (bacteria), cell division does not involve mitosis or meiosis – bacteria reproduce asexually, by a type of cell division termed by binary fission. Yeasts reproduce asexually by budding. The cell cycle is the process that all body cells from multicellular organisms use to grow and divide. The cell cycle starts when a cell has been produced by cell division and ends with the cell dividing to produce two identical cells.

3. http://biolibogy.com/cells.html 1 A bud is formed at the surface of the cell 2 Interphase – DNA and organelles replicated 3 Set of duplicated chromosomes – each set contained within a nucleus 4 One daughter nucleus migrates into the bud 5 Bud increases in size and eventually separates from parent cell – producing a new, genetically identical yeast cell Binary Fision (Bacteria) Budding (Yeast)

4. The cell cycle can be divided into stages: G1 (“growth phase” 1) - Cells prepare for DNA replication S (“synthesis”) - DNA replication occurs G2 (“growth phase” 2)- Short gap before mitosis M Mitosis (relatively short) Affected by availability of nutrients Between each stage the cell “checks” to see if it is OK to proceed to the next stage. “ Proof-reading” enzymes check the copied chromosomes for mistakes (mutations) – the cell may kill itself (undergo “suicide”) if harmful mutations are – a process known as apoptosis. Bacterial cells complete the cycle every 20 minutes. Muscle cells never complete the cycle – “terminal differentiation” Uncontrolled and repeated cell division by mitosis results in cancer (tumours) Eukaryotic cells have a well- defined cell cycle of growth and division (mitosis). The length of the cycle varies (from minutes to hours, or , longer) ending with mitosis. Each phase of the cycle involves specific activities, and varies in length from one organism to another. The Cell Cycle

5. MITOSIS (M) Process by which a nucleus divides into two – each with an identical set of chromosomes – the nuclei are genetically identical Four phases – prophase, metaphase, anaphase, and telophase Followed by cytokinesis – division of the cell into two genetically identical daughter cells INTERPHASE Period of cell growth; cell prepares cell for cell division (mitosis); genetic material (DNA) is copied and checked for errors – prevents mutations being passed on No apparent activity New organelles and proteins are made Divided into three phases (G1, S, and G2 phase) CELL CYCLE G1 S phase G2 Mitosis (M) Two daughter cells – genetically identical

6. G1 + S + G2 = INTERPHASE No apparent observable activity Cytokinesis – cell divides into two DNA content = 20 G1 - First growth phase – longest phase Protein synthesis – cell “grows” Most organelles produced Volume of cytoplasm increases Cell differentiation (switching on or off of genes) Length depends on internal and external factors If cell is not going to divide again it remains in this phase DNA content = 20 (arbitary) S - Replication phase DNA replication – this must occur if mitosis is to take place The cell enters this phase only if cell division is to follow DNA content = 40 G2 - Second growth phase - short Short gap before mitosis (cell division) Cytoskeleton of cell breaks down and the protein microtubule components begin to reassemble into spindle fibres – required for cell division DNA content = 40 The Cell Cycle

7. Mitosis occurs rapidly during growth and repair to make new cells. The rate is controlled according to the situation – e.g., during growth and repair the rate may be fast, but when growth or repair is achieved the rate declines or mitosis stops. Cancers (tumours and diffuse) are formed through uncontrolled cell division by mitosis Mitosis Mitosis occurs wherever an increase in number of cells is needed. It is important in the growth and repair of multicellular organisms, and in the population growth of unicellular organisms During mitosis a cell produces two copies of itself. Each is genetically identical to the other and to the parent cell from which they were formed. The parent cell copies its chromosomes by replication prior to cell division.

8. Chromosomes A duplicated and condensed eukaryotic chromosome with two sister chromatids Homologous Chromosomes Chromosomes occur in pairs – there are 23 pairs of chromosomes. Each pair consists of a chromosome from the male (father) and one from the female (mother). The chromosomes that make up each pair of the 22 pairs are the same size and have the same or alternative versions of genes for particular characteristics. Each individual pair of similar chromosomes is called a homologous pair.

10. Before a cell divides, its chromosomes are copied exactly in INTERPHASE. This process is called replication; ATP is synthesised – provides energy for cell division; organelles are replicated and proteins are made PROPHASE The DNA of each chromosome is copied to form two chromatids (“sister” chromosomes); chromosomes condense – becoming shorter and fatter – visible under LM; nuclear envelope breaks down; chromosomes lie freely in cytoplasm; centrioles move to opposite ends of the cell, forming protein (tubulin) fibres across it called a spindle – fibres extend to the equator of the cell METAPHASE Chromosomes line up at the equator; the spindle fibres from each pole become attached to the centromere of the chromosomes ANAPHASE The spindle fibres contract; the centromeres are split and the pairs of sister chromatids are separated and dragged to opposite poles assuming a “V” shape – the centromeres lead; a complete set of chromosomes is therefore found at each pole; energy (ATP) is required TELOPHASE Chromatids reach their respective poles and uncoil – become thin and long again – now called chromosomes again – no longer visible under LM; spindle fibres break down; nuclear envelope forms around each group of chromosomes – forming two nuclei; cytokinesis follows – cytoplasm divides and a plasma membrane forms two form two individual cells; cell enters interphase once again

11. Cytokinesis Division of the cytoplasm (cytokinesis) into two equal parts follows mitosis A “waist” forms in the middle of the cell. Eventually, the plasma membrane from one side of the cell joins that of the opposite side of the cell and the two new cells separate. The two daughter cells are genetically identical. The equator of the cell is constricted by a ring of contractile proteins (actin) in the process of cleavage, to create two cells. In plant cells, the Golgi apparatus and associated secretory vesicles assemble at the equator. Their contents are deposited to form a plate (the cell plate). Some vesicles remain intact and make connecting channels, termed plasmodesmata, through the new cell wall.

12. Prophase

13. Metaphase

14. Anaphase Telophase

15. Meiosis Meiosis is a type of cell division used in the formation of gametes (spermatozoa and ova) in animals, and spore formation (which precedes gamete formation) in plants - it is termed reduction division. Meiosis creates genetically different cells and variation within species The number of chromosomes is halved - from diploid (46) to haploid (23). Involves two distinct divisions Meiosis I – homologous pairs of replicated chromosomes are separated Meiosis II – reduction division (chromatids separated into individual haploid gametes) Four cells (gametes/spores), genetically different from each other, result from one parent cell – each cell containing half the original number of chromosome Production of haploid (n) gametes ensures the maintenance of the diploid (2n) number in subsequent generations Meiosis involves certain “mixing” events, and these, along with the random fusion of gametes in fertilisation to form a diploid zygote, results in genetic variation in the offspring which may be of adaptive advantage.

17. The DNA of each chromosome is replicated to form two chromatids. chromosomes arrange themselves into homologous pairs (both coding for the same characteristics), and prepare for cell division. At this point maternal and paternal chromatids can exchange bits of DNA to recombine their genetic material and increase the potential for variation. Homologous pairs of chromosomes separate and move to the poles of the parent nucleus. For each of the 23 pairs there is a 50-50 chance as to which pole the paternal or maternal pair of chromatids go. With over 8 million possibilities there are many opportunities for variation. Nucleus now divides to form two daughter nuclei, each with a mixture of paternal and maternal chromosomes but with half the full complement of genetic material (and no pairs at all). This division is called Meiosis 1. The two daughter nuclei themselves divide to form gametes . This second division - Meiosis 2 - works just like mitosis. The chromosomes (really pairs of chromatids) split apart to form the genetic material of the four new cells. The end result is four sex cells each with a complete but single set of 23 chromosomes. On fertilisation the nuclei of the sperm and the egg join to form a new nucleus, called the zygote . The zygote contains 23 pairs of chromosomes - 23 single chromosomes from the sperm, and 23 single chromosomes from the egg.

18. Meiosis leads to variation among the offspring of sexual reproduction in 3 ways: Crossing over – crossing over of genes from one chromatid of one chromosome to the chromatid of the other homologous chromosome Reduction and fusion of gametes from different individuals – gametes have haploid number of chromosomes – allows a gamete from one of these cells to fuse with another cell with a different haploid set, producing a zygote which has the normal diploid number of chromosomes but a new combination of genes Independent (random) assortment – when the chromosomes line up as pairs at the equator (metaphase I) of the spindle, it is by chance which “way round” each pair lies.

19. Mitosis and Meiosis Compared Mitosis Meiosis Purpose To make daughter cells identical to the parent cells - eg during growth and repair To produce sex cells ( gametes ) Takes place .. In all cells apart from gametes In the reproductive organs (ovaries and testes) Produces how many cells? Two daughter cells Four gametes What happens to number of chromosomes? Same number as in parent cell Diploid = 46 (in pairs) Half as many as in parent cell (The original number of chromosomes is restored when two gametes fuse to form a zygote .) Haploid = 23 (single) How do parent and daughter cells differ genetically? Not at all - genetic material is copied exactly (replicated) Contain a mixture of chromosomes from two parent gametes - so cannot be identical Variation between daughter cells? No - they are clones of each other Yes - they are genetically different from each other because chromosomes get shuffled up during division

20. The drugs vincristine and vinblastine from the periwinkle plant inhibit spindle assembly – used in the treatment of cancers (leukaemia and lymphomas) and gout Taxol form the Pacific ewe inhibits depolymerisation of spindle fibres (de-assembly), which effectively stops cell division – used to treat ovarian cancers

21. Stem Cells Multicellular organisms function as a result of many different cell types that are specialised for their function – e.g: Neurones (nerve cells) – specialised for the transmission of electrical nerve impulses Red blood cells (erythrocytes) – specialised for the carriage of oxygen aroung the body Vascular tissue (xylem and phloem) in plants - cells in the cambium tissue of plants differentiate to form vascular tissue – specialised for transport All cells in multicellular organisms originate from stem cells. These are unspecialised cells that divide to become new cells, which then differentiate to become specialised into different cell types. Differentiation is achieved due to the switching on and off of relevant genes