prezentare power point herpes bla bla bla :)

1. HERPESVIRIDAE herpein: Greek: to creep

2. Herpes simplex 1 and 2 (To “The sound of silence” Simon and Garfunkle) Hello, herpes, our old friend Will be with us to the end. ‘ Cause the virus softly creeping Left its genes while we were sleeping Not integrated into our genome, It will roam that episome. The DNA of herpes.

4. The case of the elephant herpesviruses (Richman et al. 1999. Science 283:1171) Asian elephant herpesvirus (“cold sores”) Fatal hemorrhagic Disease in African elephants African elephant herpesvirus (“cold sores”) Fatal hemorrhagic Disease in Asian elephants

5. The elephant herpesvirus Richman et al. 1999. Science 283:1171

6. Viruses with ds DNA genomes Poxviridae African swine fever virus Herpesviridae Adenoviridae Papovaviridae bovine herpesvirus-1,2 porcine cytomegalovirus equine herpesviruses -1,4 malignant catarrhal fever virus

7. CLASSIFICATION

8. CLASSIFICATION

9. GENERAL CHARACTERISTICS

10. Herpesvirus Family Tree (Genetic) CLASSIFICATION GAMMAHERPESVIRUSES EHV2 HVS HHV8 EBV HSV1 HSV2 EHV1 PRV VZV ALPHAHERPESVIRUSES BETAHERPESVIRUSES HHV7 HHV6 HCMV

11. Structure nucleocapsid envelope tegument glycoproteins (gB, gC…gL)

12. GENERAL CHARACTERISTICS

13. GENERAL CHARACTERISTICS

14. BIOLOGICAL PROPERTIES

15. REPLICATION The genome structure of herpesviruses: (A) Alphaherpesvirus genomes comprise two regions designated long (L) and short (S). Terminal repeat (TR) and internal repeat (IR) sequences may bracket unique sequences (U L , U S ) of both L and S or only S. Repeat sequences are shown as boxes and are encoded as indicated by the direction of the arrows. Repeat sequences allow the DNA they bracket to invert relative to the rest of the genome such that where both U L and U S are bracketed by repeat sequences, four isomers are made and packaged in equimolar amounts into virions. Where only S is bracketed by repeat sequences, two equimolar isomers are made. (B) The genome of equine herpesvirus 2, a betaherpesvirus, contains terminal direct repeat structures. (C) The genome of bovine herpesvirus 4, a gammaherpesvirus, contains multiple direct terminal repeat sequences (small boxes) in a nonequal, variable number of copies.

16. Replicative cycle inclusion body cell-cell spread

17. REPLICATION Diagram representing transcription, translation, and DNA replication of a typical herpesvirus. Transcription and posttranscriptional processing occur in the nucleus, translation in the cytoplasm, and some of the  and  proteins are involved in further transcription and some  proteins in DNA replication.

18. Productive and latent infections

19. Viral DNA Latency and Reactivation LAT IE genes (regulatory) E genes (enzymes) L genes (structural) stress immunosuppression corticosteroids cAMP reactivation

20. Herpesviruses That Cause Diseases in Domestic Animals

21. DISEASES CAUSED BY HERPESVIRUSES

22. Bovine herpesviruses

24. Infection in the animal reactivation from latency replication in epithelial cells (rhinotracheitis) Infection of susceptible animal systemic cell-associated spread infection of the fetus -> abortion encephalitis replication in epithelial cells establishment of latency (infection for the first time)

25. Primary lesion

26. Clinical and immunological parameters

31. Intranasal vs intramuscular vaccines Route of Inoculation Advantages Disadvantages Intra nasal Stimulates mucosal and systemic immunity. Stimulates interferon. Less affected by maternal antibody. Immunity not as long-lasting as IM. Serum antibody titres lower. Difficult to administer. Intra muscular Longer lasting, higher levels of immunity. Susceptible to maternal antibody.

33. Vaccines for eradication gB gB gB gB gene for gE deleted gE gB infected vaccinated ELISA for antibodies infected vaccinated gD subunits gE gD gD gD

34. DISEASES CAUSED BY HERPESVIRUSES

35. PRV - Aborted Fetuses DISEASES CAUSED BY HERPESVIRUSES

36. Neonatal: Infection DISEASES CAUSED BY HERPESVIRUSES

37. CNS Signs DISEASES CAUSED BY HERPESVIRUSES

38. PRV Encephalitis - in situ DISEASES CAUSED BY HERPESVIRUSES

39. PRV - Pneumonia DISEASES CAUSED BY HERPESVIRUSES

40. PRV - Infected tonsils - in situ DISEASES CAUSED BY HERPESVIRUSES

41. DISEASES CAUSED BY HERPESVIRUSES

42. DISEASES CAUSED BY HERPESVIRUSES

44. Pruritis in Mice DISEASES CAUSED BY HERPESVIRUSES

45. DISEASES CAUSED BY HERPESVIRUSES

46. DISEASES CAUSED BY HERPESVIRUSES

50. Pathogenesis of EHV-1 abortions: bronchioles EHV infects endothelial cells and leukocytes cell-associated viremia IL2 induces Adhesion molecules inflammation Thrombotic occlusion vasculitis Virus infects fetus Endometrial vessel

52. efferent lymphatic EHV-1 epithelial invasion and generation of viremia Infected endothelial cells in epithelial lamina propria Infected T lymphocytes in drainage lymph nodes Viremia Infection of epithelium and lamina propria (Kydd et al., 1994)

56. Pathogenesis of abortion viremia endometrial endothelial cell infection endometrial vasculitis and thrombosis

57. Pathogenesis of abortion viremia endometrial endothelial cell infection endometrial vasculitis and thrombosis extensive infarction: virus negative fetus

58. Pathogenesis of abortion viremia endometrial endothelial cell infection endometrial vasculitis and thrombosis extensive infarction: virus negative fetus limited infarction: virus positive fetus

62. DISEASES CAUSED BY HERPESVIRUSES

65. DISEASES CAUSED BY HERPESVIRUSES

66. DISEASES CAUSED BY HERPESVIRUSES

68. DISEASES CAUSED BY HERPESVIRUSES

70. DISEASES CAUSED BY HERPESVIRUSES

71. malignant catarrhal fever (gamma herpesvirus) other ruminants ? other species malignant catarrhal fever

73. acute MCF

74. IMMUNE RESPONSES TO HERPESVIRUSES

75. IMMUNE RESPONSES TO HERPESVIRUSES

76. IMMUNE RESPONSES TO HERPESVIRUSES

77. IMMUNE RESPONSES TO HERPESVIRUSES 1 st Generation  - Conventional killed - Modified-live - e.g. PR- Vac, Pseudo vax 2 nd Generation  - Gene-deleted, i.e. virulence genes - e.g. Omni Vac-1 3 rd Generation - Gene-deleted, i.e. differential marker - e.g. PRV-marker, Tolvid Omnivac II 4 th Generation  - Multiple marker genes - e.g. PRV-Gold ?? 

78. IMMUNE RESPONSES TO HERPESVIRUSES