Diagnosis
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Diagnosis
- 1. LABORATORY DIAGNOSIS OF PARASITIC INFECTIONS By: Dr.Maha jaafar
- 3. DIAGNOSIS DIRECT INDIRECT MOLECULAR Urine Stool Sputum Biopsy Blood Aspirates PCR DNA probes IHAT LAT IFAT ELISA CFT DEIDT
- 4. Acetic acid RBC haemolysis Clear ova Ether Dissolve fat M.f URINE EXAMINATION MACROSCOPIC MICROSCOPIC colour white smoky Sedimentation concentration Membrane filtration Chyluria Blood Filaria S. haematobium
- 6. URINE EXAMINATION Membrane filtration technique air 10 ml urine Nucleopore filter Eggs of Schistosoma + Saline
- 15. STOOL EXAMINATION Saline sedimentation 10 g stool Saline Mesh wire gauze Conical flask Sediment Emulsify
- 22. STOOL EXAMINATION Kato technique Mesh screen Template Hole Remove the template Cellophane soaked by glycerin (clears faeces( Egg count/ g stool Egg quant. Of: Ascaris, T. trich., Hookworms, S. mansoni
- 23. STOOL EXAMINATION Stoll’s technique NaOH 4 g Stool Erlynmeyer flask 56 CC 60 CC Shake well 0.15 CC Egg count/ slide Eggs/1g= Eggs/slideX100 Egg/day=Eggs/1g X stool wt/g in 24 hrs 24 hr stool Egg quant. Of: Ascaris, T. trich., Hookworms, S. mansoni
- 26. DIAGNOSIS DIRECT INDIRECT MOLECULAR Urine Stool Sputum Biopsy Aspirates Blood PCR DNA probes IHAT LAT IFAT ELISA CFT DEIDT
- 27. NaOH Sputum Centfifuge SPUTUM EXAMINATION MACROSCOPIC MICROSCOPIC Appearance Concentration Bloody (Parag) Rusty brown (Parag)
- 31. DIAGNOSIS DIRECT INDIRECT MOLECULAR Urine Stool Sputum Biopsy Aspirates Blood PCR DNA probes IHAT LAT IFAT ELISA CFT DEIDT
- 32. BLOOD EXAMINATION Blood films Buffy coat films QBC technique Knott’s conc. tech. Thin Thick
- 34. BLOOD EXAMINATION Buffy coat film centrifuge RBC WBC (BC) plasma Citrated bld 30 min Air dry Fix spread Geimsa Tryp., L. donovani
- 35. BLOOD EXAMINATION QBC technique centrifuge RBC RBC +parasite Microhaematocrit tube Acridine orange Malaria, Filaria, Trypanosomes
- 40. INDIRECT IMMUNOLOGICAL METHODS INDIRECT FLUORESCENT ANTIBODY TEST parasite Patient’s serum (?? AB) Anti human AB fluorescein
- 41. INDIRECT IMMUNOLOGICAL METHODS ELISA OPD OPD Flat bottom plastic micrititre plate Ag Patient’s serum (?? AB) Anti human AB Peroxidase E AB
- 42. INDIRECT IMMUNOLOGICAL METHODS CFT Ag Patient’s serum (?? AB) complement Anti sheep AB Sheep’s RBC -ve Ab +ve Ab haemolysis No Sheep RBChaemolysis Tube / microplate AB
- 43. INDIRECT IMMUNOLOGICAL METHODS Double Electro Immuno Diffusion +ve -ve Ag Ab Buffered gel Electric current Line of ppt
- 44. INDIRECT IMMUNOLOGICAL METHODS Immunodiagnostic Strip Test (Dip Stick Test) Ag Nitrocellulose strip Monoclonal Ab Coloured dye Pt bld (?Ag) +ve -ve Malaria, Filaria, African tryp.
- 45. DIAGNOSIS DIRECT INDIRECT MOLECULAR Urine Stool Sputum Biopsy Blood Aspirates PCR DNA probes IHAT LAT IFAT ELISA CFT DEIDT
- 46. MOLECULAR BIOLOGICAL TECHNIQUES DNA Probes DNA Probe Commercially prepared DNA sequence Radio active material Nitrocellulose paper Sample (Serum/ stool) ?? parasite +ve parasite Hybridization Radioactivity
- 47. MOLECULAR BIOLOGICAL TECHNIQUES Polymerase Chain Reaction (PCR) Single stranded DNA Replication Detection T cruzi, T gondii