1. Into the lab
DEG, SSH, degenerative PCR
Lecture
FISH 507a Bioinformatics
2. Stuff
• Homolog Project
• Query Files
• question: movies
• extra credit
2
3. Taking the molecular approach
• evolution
– comparing sequences, markers
• physiological differences
– comparing phenotypes
• differences in expression level
– first have to find the genes
3
5. How do you find genes
• Entrez (qualifers)
• Sequence similarity (BLAST)
• Large scale BLAST on ESTs
• Degenerative PCR
• SSH, DEG
• Sequencing (can include SSH etc)
5
6. Top down approaches to Gene Discovery
Differential Display and Suppression Subtraction
Useful for characterizing transcriptome differences in
different treatments, tissues, stages of development
when there is limited genomic information or a desire to
use a non-hypothesis gene target model.
As opposed to…..