The document summarizes several experiments studying the bacteria Vibrio tubiashii. Key findings include:
1) Protein gel and RNA isolation results found more proteins and RNA present when V. tubiashii was grown in the presence of clam foot tissues compared to without tissues.
2) qPCR results found higher levels of certain V. tubiashii genes expressed when bacteria were grown in the presence of oyster hemocytes compared to their absence.
3) Exposure experiments varying pH levels and presence of live vs autoclaved oysters showed some effects on V. tubiashii protein and gene expression over time.
![Foot Experimet ,[object Object],Protein Gel: distinct bands in the samples without foot; smear in the samples with foot, but differnt size bands evident- possible proteases Gel was repeated – same results Isolation of the RNA: There is significantly more RNA in the foot samples mass of the pellet: VT+foot-------0.86g VT no foot---0.22g Spectrophotomer analysis: VT+foot-------1669.37 ng/ul Vt no foot-----351.01 ng/ul Both samples were diluted to the concenration of app. 150 ng/ul PCR of the samples: Primers used: 1. Vtubi_16s 2. Vtubi_chitinase 3. Vtubi_vthB VthB and Chitinase produced bands. Interesing absence of 16s bands](data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7)