4. Introduction Each kind of vegetables has its own specific nutrition and benefit. The vegetables under our study are Chilli ( Capsicum frutescens L. var. frutescens ) , Celery ( Apium graveolens L.) , Parsley ( Erygium foetidum Linn. ) , Dill ( Anethum gaveolens L.) and Bitter Cucumber ( Momordica charantia L.) . All of which are abundant and easy to obtain in the Salaya area and, moreover, the study in the level of tissue of these plants are not widely known
5. Introduction Since all the study in anatomy of plants are always on the basis of tissue of these plants are not widely known. Since all the study in anatomy of plants are always on the basis of tissue level study, the data of this project can be used as the basis of other kinds of studies regarding vegetables, such as the study of the relation between the plants’ structure and the ability to collect antioxidant substance.
30. Methods Search for information Select and preserve the plant specimens in FAA Dehydrate the tissue by preserving it in Tertiary Butyl Alcohol (TBA)
31. Methods Preserve the tissue in melting paraplast at 60 o C Embedding Cut it with rotary microtome Stretch the specimens by using 3% formalin and Haupt’s decisive
32. Methods Staining with Safrinin O and Fast green Wash the stain out Mounting with mounting medium (Permount), Drop it and cover it with cover glass
43. Plants which people admire to eat their leafs, celery had larger area of dermal tissue than dill and celery, respectively. While the ratio of ground tissue area and vascular tissue area, celery had much value than celery and dill, respectively. In another group that people admire to eat their fruits, chili had larger area of dermal tissue than bitter cucumber. But bitter cucumber had more value of ratio of ground tissue area and vascular tissue area than chili.
44. References Consortium of Thai Medical Schools. 1997. Local Plant. The War Veterans Organization Press, Bangkok C. Brickell. 2003. The Royal Horticultural Society A-Z of GARDEN PLANT volume1: A-J. Dorling Kindersley L imited, London. L. Bremness. 1994. HERBS. Dorling Kindersley Limited, London. L. Bremness. 2000. HERBS. Dorling Kindersley Limited, London. Faculty, Kasetsart University, Bangkok
45. References Prof. Ass. Dr. Prasart Keurmanee. 2006. Plant Tissue Technique. Botany Department, Science Faculty, Kasetsart University, Bangkok R.F. Evert. 2006. Esau’s Plant Anatomy 3rd edition. John Wiley & Sons, Inc., New Jersey. Rost T . L . , M . G . Barbour, C . R . Stocking, and T . R . Murphy . Plant Biology . 2nd edition . Thomson Higher Education, USA .
46. References Sylvia Lee-Huang, Paul L. Huang, Philip L. Huang, Aldar S. Bourinbaiar, Hao-Chia Chen and Hsiang-Fu Kung. 1995. Inhibition of The Integrase of Human Immunodeficiency Virus (HIV) Type 1 by Anti-HIV Plant Proteins MAP30 and GAP31. M.S. thesis, Department of Biochemistry, New York University School of Medicine, New York, NY 10016 ; tAmerican BioSciences, New York, NY 10021 ; INational Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 ; and IlLaboratory of Biochemical Physiology, Biological Response Modifiers Program, Division of Cancer Treatment, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD. Faculty, Kasetsart University, Bangkok