9. Microfluidique#
• Manipula>on#de#liquides#et#de#par>cules#par#
champ#électrique#:#micropompes,#mélangeurs#
• Manipula>on#de#cellules##
• Manipula>on#de#cellules#
• Séquenceurs#d’ADN#(Puces#à#ADN)#
me of normal saline
mally extracted ISF
o establish electrical
le it is transferred
me of normal saline
E3, shown as the red
e extraction chamber
ctrode pair E3 and
ves. And the volume
blue curve in Figure
)
43 tt
S
−
+ , (1)
crochannel between
the width and height
time for the head of
e pair E1 and E2, t3
ple flowing through
0mm) is the distance
. The volume of
to the difference
efined input volume
ensor for controlling
asy collection of ISF
Collection Chamber,
Saline Chamber, D:
Saline. V1-V3 are
rs of electrodes.
lucose Sensor
concentrations of the
S dielectric glucose
ated electrode and a
acitive detector, is
tem. As shown in
s situated inside a
se-sensitive polymer
a semi-permeable
tween glucose and
) causes permittivity
solution, which is
r to determine the
ISF Transdermal
cose Sensor
top layer of the ISF
volume sensor for
n. A microchamber
(Figure 5) is aligned between the extraction chip and the
glucose sensor for containing the collected ISF sample in
glucose concentration measurement step.
Figure 3: Schematic of the dielectric glucose sensor.
Figure 4: The polymer composition and the mechanism of
interaction with glucose.
Figure 5: Schematic of the integration structure between
ISF transdermal extraction chip with volume sensor
(TECVS) and MEMS dielectric glucose sensor. APGS:
Access Ports for Glucose Sensor.
FABRICATION PROCESS
The microfluidic chip without glucose sensor, which
is designed for ISF transdermal extraction, collection, and
volume measurement, is fabricated from five PDMS layers
using micromolding techniques. 250 m thick epoxy dry
films (SUEX TDFS, DJ DevCorp) and photoresist films
(FX930, DuPont) instead of common photoresists are
utilized for micromolds fabrication. As shown in Figure 6a,
the electrodes of the volume sensor are fabricated with
conductive PDMS (Ag- P/PDMS, 11:2 weight ratio)
similar to the method utilized by Niu et al. [6]. After
molding, the five PDMS layers are aligned and bonded
together using Corona (Laboratory Corona Treater,
BD-20ACV, Electro-Technic Products, Inc.).
For the fabrication of the MEMS dielectric glucose
sensor (Figure 6b), two gold layers are firstly deposited,
patterned, and passivated with Parylene to form the bottom
electrode and perforated electrode. And a sacrificial
photoresist layer is deposited and patterned between the
two gold layers. Then two SU-8 layers are deposited and
patterned to form the diaphragm and the sensor chamber.
366
Mesure#de#glycémie#sur#puce#
Transducer#2013,#Li#et#al.#
#
33#
Microfluidique#
34#
Microfluidique#
• Domaine#scien>fique#rela>vement#nouveau#:##
– Comportement#de#liquides#à#volumes#réduits#
– Interac>on#avec#le#champ#électrique#
– Technologies#(biocompa>bles#?)##
• Domaine#en#plein#essor##
35#
MEMS#pour#op>que:#MOEMS#
• Domaine#:#traitement#d’informa>on#
op>que,#principalement#dans#les#télécoms#
• #Interrupteurs,#miroirs##
Interrupteur#op>que##
Sélec>onneur/mul>plexeur#
op>que#à#microImiroirs# 36#