The document analyzes regulatory elements in pathogenic genes of Pseudomonas syringae Pv. tomato DC3000 and Mycobacterium tuberculosis H37Rv. Gene expression data and clustering analysis were used to identify genes that are likely coregulated. Upstream regions of clusters containing virulence genes were analyzed for regulatory motifs like promoters and transcription factor binding sites using tools like RSAT. The analysis found most gene clusters in the two bacteria have upstream regions 50-500bp in length. M. tuberculosis was found to have more dyad patterns than oligo-patterns in the upstream regions compared to P. syringae. The results provide insight into gene regulatory networks of pathogenic genes in these bacteria.

1. Under the guidance of :- Mr. Pramod Katara Presented by :- Supriya Karkra Ganga Jeena Ishu Sharma Mugdha Agarwal Prediction of Regulatory Elements for Pathogenic Genes of P. syringae Pv. tomato DC3000 and M. tuberculosis H37Rv.

3. Introduction

9. Review of Literature

11. Materials and Methodology

13. Search for Virulent Genes P.syringae Pv. tomato DC3000 M.tuberculosis H37Rv Domain of Virulent Genes Domain of Virulent Genes SMD Data Clusters Phlyogenetic Tree Clusters Clusters of Probable virulence genes RSAT Upstream region Oligo-nucleotides Spaced Dyads

14. Results

21. http://genome –www5.stanford.edu/ http://www.tigr.org/ http://rana.lbl.gov/ http://www.mgc.ac.cn/VFs/ http://rsat.ulb.ac.be/rsat/ http://www.phylogeny.fr/ http://ncbi.nlm.nih.gov/ URL References :

22. THANKS