Cory Klemashevich Resume - PhD Chemical Engineering
Margaret Jewell Cv
1. Dr MARGARET JEWELL
PhD (Genetics); B. Agr. Sc. (Hons 1)
120 Ardoyne Road, Oxley, QLD 4075
M: 0432 395 170E:m.jewell@uq.edu.au
An outgoing and enthusiastic genetic technologist
Highly experienced in advanced PCR, SGS/NGS, qPCR/SDS
Awarded UQ Deans Academic Achievement (2001to 2006)
Pursuingspecialist roles in Next Generation Sequencing
PROFILE
A proven scientific researcher, technician, communicator, and support-person
A sharp, practical,analyticaltroubleshooterable to think both laterally and logically
A fast learner, highly adaptable, and enthusiastic member of the team
Diplomatic, hardworking and loyal, with exemplary interpersonal skills
Positive work ethic, reliable, confident and self–motivated
Multi-tasker with demonstrated ability to satisfy personal ambition and employer success
PROFESSIONAL ASSETS
Natural ability to verbally communicate with fellow staff, clients, and collaborators
Natural ability to communicate written information effectively
Excellent organisation and time management skills
Demonstrated ability to work under pressure and meet demanding deadlines
Facilitation, collaboration, and training experience from scientific and consulting roles
Experience and appreciation of field work, work-related travel, and customer relations
Extremely high level technical scientific computer and analytical skills
Natural Ability to analyse large environmental and molecular datasets in
EDUCATION
PhD in Genetics awarded in May University of Queensland 2012
Bachelor of Agricultural Science Honours Class 1 University of Queensland 2006
High School Certificate (Year 12) OP 8 Brisbane State High School 1994
KEY ACHIEVEMENTS
Undergraduate GPA of 6.8, with Honours Class 1
Academic Awards :
» EUCARPIA 29th Fodder Crop and Amenity Grasses Young Researcher Bursary 2011
» AW Howard Memorial Trust Research Fellowship recipient 2010
» Australian Postgraduate Award – Industry 2008-2011
» Eight Commendations for High Achievement (2001 to 2006)
» The Robert Philip Scholarship 2006
» The Downing Brothers Bursary 2006
» The Bell Memorial Medal 2006
» The E R Butler Prize 2006
PhD thesisawarded in May, 2012. The title„Genetic, ploidy level, and species variation of
CynodonL. C. Rich (Poaceae) in Australia’
Fivepublications in peer reviewed scientific literature
2. Margaret Jewell2
CAREER SUMMARY (RECENT)
University of Queensland (Sch of BiolSci) Postdoctoral Research Fellow Jul 2012 - Current
Duties and Achievements:
ChIP-seq, RNA-seq, and qPCR design, preparation, experimentation and analysis (sequence
detection systems)
Supervision of undergraduate students
Development of strong understanding of next-generation sequencing technologies and
platforms, particularly with regard to ChIP and RNA sequencing in plants.
Liaising with sales representatives and ordering laboratory consumables, reagents, and
equipment.
University of Queensland (Sch of Agri& Food) Research Officer Nov 2011 –July 2012
Duties and Achievements:
TILLING (Targeting Induced Local Lesions in Genomes) design and analysis
High throughput PCR, DNA pooling, and genotyping
PCR optimisation across pooled DNA
Student supervision in field and laboratory (incl. planting, maintenance, harvesting, DNA
extractions, PCR, gel electrophoresis) for a genetic segregation experiment
Research and identification ofmetabolic genes and proteins using bioinformatics software
Planting, harvesting, and threshing of several large scale field plots of Sorghum
University of Queensland (Sch of BiolSci) Research Officer Nov 2011 –July 2012
Duties and Achievements:
Assisted PhD and honours students carry out ecological field work, such as experimental set
up, transplanting, measuring, and maintenance
Planned and implemented a large-scale Senecio transplant experiment
University of Queensland (Sch of BiolSci) Tutor Mar 2009–July 2012
Duties and Achievements:
Taught and facilitated completion of practicals for 16 students per 3-hourly sessions in 1styear
university courses (BIOL1020: Genes, Cells, and Evolution; and BIOL1040: Cells to Organisms)
o PCR, digestion, cloning, transformation, pipetting, sterile technique, pedigree analysis,
genetic inheritance, photosynthesis (C3, C4, CAM), plant vascular systems,
Marked and provided feedback for exams, assignments, and practical worksheet-based assessment
The University of Queensland (Sch of Agri&Foood) RHD-APAI Jul 2007 – May 2008
Duties and Achievements:
High throughput DNA extraction and maintenance of large databases
Developed multiplex PCR reactions using EST-SSRs
Flow cytometry and root tip squashes (incl. microscopy) for ploidy determination
High throughput genotyping, fingerprinting, and sequencing analysis
Creativey analysed relationships between climate, geography, ploidy and genetic variability
Programming and Software: PYTHON, R, IMAGING, GENEMAPPER, FACSDIVA, MODFIT,
GENEIOUS, EXCEL, DIVA-GIS, DARWIN, STRUCTURE, TESS, PAUP, MESQUITE, Mr BAYES, NTSYS,
MEGA, DNASP, GARLI.
Lloyd Consulting Environmental consultant Jul 2007 – May 2008
Duties and Achievements:
Participated in the development of the Cabbage Tree Creek Local Integrated Water Cycle Plan
Assisted in development of the Brisbane Citywide Integrated Water Cycle Plan
Assistend in development of an EIS for a large new development south of Townsville
Developed technical documents including
» Proposals in response to advertised tenders
» Third party review reports for contaminated site assessments
» Environmental Management Plans for mines and refineries
» Quarterly monitoring reports for waste management facilities and transfer stations
Gained experience in project management systems and business management systems; and
Attended meetings with Council and other clients to discuss projects
3. Margaret Jewell 3
CSIRO Plant Industry Research assistant (Placement) Jul 2005 – Aug 2005
Duties and Achievements:
DNA extraction, buffer preparation, leaf grinding using liquid nitrogen, centrifugation, PCR
using a radioactive 33P label,agarose and acrylamide electrophoresis, PCR analysis, cDNA library
development, field collections
RESEARCH SKILLS
Producing published documents and reports Communication with academic staff and the
for use in the scientific community wider research community
Planning and performing experiments within Developing tests to evaluate project
research laboratory environment performance
Independent learning and research Extensive computer-based analytical skills
Using genetic data analysis software Project management
Planning & conducting laboratory work Presenting at international conferences
Recording sample details Radiation safety training
Immediate laboratory workplace safety Wider work environment OH&S
LABORATORY SKILLS
Next Generation Sequencing (ChIP- Molecular diagnosis Flow cytometry
seq, RNA-seq) DNA and RNA extraction Transformation
Second Generation Sequencing Sterile techique Cloning
PCR (high throughput) Restriction digestion Virology
Genotyping (high throughput) Reverse transcription Microscopy
Sequencing (high throughput) Radioactive labelling Tissue culture
Electrophoresis (agarose and SDS) qPCR and SDS Histology/staining
PUBLICATIONS
Published
M. C. Jewell, Y. Zhou, Duc The Ngo, D. Loch, I. D. Godwin, and C. J. Lambrides (2011). “Maximising genetic,
morphological, and geographic diversity in a core collection of Australian bermudagrass”. Crop Science 52:
879-889.
M. C. Jewell, B. C. Campbell, I. D. Godwin (2010)” Transgenic plants for abiotic stress resistance.” In: Kole
C, Abbott AG, Michler CH, Hall TC (eds) Transgenic Crop Plants: utilization and biosafety. Springer, Berlin, pp
67-132.
M. C. Jewell, C. H. Frere, P. J. Prentis, C. J. Lambrides, I. D. Godwin (2010) “Characterisation and
multiplexing of EST-SSR primers in Cynodon (Poaceae) species”. American Journal of Botany Primer Notes
and Protocols 97:e99-e101
R. Kearns, Y. Zhou, S. Fukai, C. Ye, D. S. Loch, I. D. Godwin, T. A. Holton, D. Innes, H. Stirling, N. Cao, M. C.
Jewell, C. J. Lambrides (2009) “Eco-Turf: water use efficient turfgrasses from Australian
biodiversity”.ActaHorticulturae 829:113–118.
M. C. Jewell, C. H. Frere, K. Harris-Shultz, W. F. Anderson, I. D. Godwin, C. L. Lambrides (2012).
“Phylogenetic analysis reveals multiple origins of Cynodonspecies in Australia”. Molecular Phylogenetics
and Evolution. doi: 10.1016/j.ympev.2012.06.026
Accepted for publication
Christopher J Lambrides, Yi Zhou, ShuFukai, Margaret Jewell, Don Loch, Ian Godwin, Tim Holton, David Innes,
Celine Frère, Wayne Hanna, Changrong Ye, Thinh Van Tran, The Ngo, Thu Le, Ninh Cao, Haider Al Dabbagh,
Chris O‟Brien, Matt Roche, Garry Fullelove, Emma Ballard, Ryan Kearns, Will Pearce, Andrew Hacker,
MeenaPuachuay, Max Shelton, Scott Dalzel, Bill Anderson, Karen Harris-Shultz, Julia Cremer. “EcoTurf- a Case
study: Genetic variation and agronomic potential of bermudagrass (Cynodon spp.) germplasm collected
from Australian biodiversity”
In preparation
M. C. Jewell, C. H. Frere, P. J. Prentis, D. S. Loch, W. W. Hanna, D. Innes, T. A. Holton, I. D. Godwin, C. J.
Lambrides (2011). “Elucidating drivers of genetic variation and population structure within species across
managed and wild environments”
4. Margaret Jewell 4
CONFERENCES
The 4th Annual Life Technologies Sequencing Summit, 6-9th October, 2012, Bali, Indonesia.
EUCARPIA 29th Fodder Crops and Amenity Grasses Section Meeting, 4-8th September, 2011, Dublin –
presentation and poster.
OzBio: The Molecules of Life – From Discovery to Biotechnology, 26th September to 1st October,
2010, Melbourne – poster.
PROFESSIONA DEVELOPMENT
Applied Markets (AgBioMetagenomics) Workshop, 9 thOctober, 2012, Bali, Indonesia
The University of Queensland‟s NCI-SF/Barrine cluster for bioinformaticians training session,
31stAugust, 2012, University of Queensland
2012 Winter School in Computational and Mathematical Biology from 2-6 July, 2012, Queensland
Biosciences Precinct University of Queensland
MEDIA
Scope, episode 2/033: More Scientists at Work – Agricultural Scientist (http://ten.com.au/scope-
18070.htm).
ADDITIONAL INFORMATION
Status : Australian citizen
Interests : Cycling, gardening, art & craft (previously professional mosaicist), family
Affiliations : Australian Institute of Agricultural Science and Technology, International
Wheat Genome Sequensing Consortium
Travel : USA, Europe, Africa, Asia
Community : Redhill Paddington Community Centre, paid & volunteer work & fund raising
Computer : Microsoft (Word, Excel, Power Point, Outlook), Genetics analysis software,
Statistical packages
REFEREES
Profess or Ian Godwin Dr Milos Tanurdzic (Current supervisor,
(Principle PhD Supervisor) conflict of interest if contacted)
School of Agriculture and Food Sciences, (Employer/supervisor, Postdoc position)
The University of Queensland School of Biological Sciences
Tel:(w) +61 7 3365 2141 (m) 0409 582 481 The University of Queensland
Email: i.godwin@uq.edu.au Tel: (w) +61 7 3365 2045 (m) 0427 537 819
Email: m.tanurdzic@uq.edu.au
Dr Daniel Ortiz-Barrientos Lois Eden
(Employer, Research Assistant Position) (BIOL1020 and BIOL1040 Practical Co-ordinator)
School of Biological Sciences, School of Biological Sciences,
The University of Queensland The University of Queensland
Tel: (w) +61 73365 1103 (m) 0403 501 826 Tel: (w) +61 73365 2992 (m) 0407 769 569
Email: d.ortizbarrientos@uq.edu.au Email: l.eden@uq.edu.au
Dr Edward Gilding Dr Chris Lambrides
(Supervisor, Research Officer position) (PhD Supervisor)
School of Agriculture and Food Sciences, School of Agriculture and Food Sciences,
The University of Queensland The University of Queensland
Tel: (w) +61 73365 3447 (m)0430 107 696 Tel: (w) +61 73365 1103 (m) 0408247 721
Email: e.gilding@uq.edu.au Email: chris.lambrides@uq.edu.au
The Honourable Patrick Keane Trevor Lloyd
Chief Justice of the Federal Court of Australia (Employer)
Tel: (w) +61 73248 1100 Lloyd Consulting Pty Ltd
Email: qldreg@fedcourt.gov.au Tel: (w) +61 7 3352 7300
Email: trevor@lloydconsulting.com.au
5. Margaret Jewell
120 Ardoyne Road
Oxley, QLD, 4075
Tel: 0432 395 170
E: m.jewell@uq.edu.au
27th October 2012
Kari Vaughan,
Recruiter, HR Department
Life Technologies
30-32 Compark Circuit
Mulgrave, Melbourne, VIC, 3170
Tel: 03 9262 3754
E: Kari.Vaughan@lifetech.com
Dear Ms Vaughan,
When I was conducting my PhD in Genetics, I interacted with Field Application Specialists (FASs) who
helped me troubleshoot instrumentation, software, and other research obstacles. These interactions were vital
for the success of my research and introduced me to an exciting career option – FAS. My PhD was awarded
in May 2012 and I have continued working in plant genetics since submitting my thesis in November 2011. I
am currently working as a postdoctoral researcher in wheat epigenetics. My background has given me great
appreciation for the rapid advancement of second and next generation sequencing (NGS) technologies and,
for many years now, my career goal has been to specialise in the NGS field. Therefore, I would like to apply
for the position of Field Application Support Specialist – NGS with Life Technologies (LifeTech).
LifeTech has been my company of choice since the Ion PGMTM sequencing platform was first introduced. I
am in awe of, and believe in, this transformative technology that is based simply on reading the pH of ions in
tiny micro-wells. Furthermore, the introduction of the Ion ProtonTM, demonstrates how fast the NGS
technologies pioneered by your companies are advancing. I believe these, compared with competitor
platforms, have the potential to dramatically increase the output of all scientists; from staff of multi-national
companies to university students. I understand the science behind competitor platforms (such as Illumina and
454). However, in my opinion, the PGM, Proton, and 5500 (SOLIDTM), combined with the Ion-AmpliSeqTM,
ChefTM, and AvalancheTM, and LifeTech kits and analysis solutions, offer the most comprehensive and
streamlined workflows. I would be extremely proud to champion these products for LifeTech. Lastly, having
met many of your team through work, meetings, and at the Sequencing Summit in Bali, I know that your
team is extremely knowledgeable and friendly and it would be my pleasure to work with them.
My resume provides details of my advanced biological experience, communication skills, and high-level
computer skills. I would also bring the following attributes, skills, and experience to your team:
My knowledge of competitor platforms and strong belief that LifeTech platforms are superior will
enable me to help sell and demonstrate your products with honesty and integrity;
My love of travel and engaging with people will enable me to conduct work wherever it is required;
I have extensive plant genetics experience and, because your clients are predominantly biomedical
and clinical scientists, my skills and fresh ideas regarding workflows could encourage other plant
scientists to adopt your platforms, expanding your client base further in the plant sciences market;
I gain great satisfaction through helping people and my experience with troubleshooting complex
scientific problems (from pre-lab to analysis) will enable me to listen to scientist’s needs and help
them achieve their goals efficiently using LifeTech platforms; and
I am extremely friendly, trustworthy, and outgoing and would thrive in an environment that enabled
me to interact with a broad scope of workers.
In addition to the above, I am a very honest and reliable worker, I enjoy a busy work environment, I will
cheerfully apply myself to any task, and I am a committed, motivated, and extremely loyal employee.
Thank you very much for giving me the opportunity to apply for this position. As you know from our email
communications, I am very excited about this role. I would gratefully accept the opportunity to meet with
you at your earliest convenience. If you would like to contact me, please phone me on 0432 395 170.
Sincerely,
Margaret Jewell