1. Production of S-containing Compounds (H 2 S, SO 2 , mercaptans, DMS) by Yeasts

3. INTRODUCTION OF TERMS and DESCRIPTORS H 2 S - Hydrogen Sulfide rotten eggs SO 2 - Sulfur Dioxide SO 4 - Sulfate HSO 3 - Sulfite Also consider that Salts containing the HSO 3 − ion are termed bisulfites Disulphides = represent the 'latent' form of sulphides in wine

4. More terms.......... Mercaptans – also known as thiols – if hydrogen sulfide isn't removed quickly can result in mercaptans – cabbagey, burnt rubber, struck flint Sulfur analogues of alcohols, -SH. Named by adding - thiol to alkane name. The -SH group is called mercapto . DMS – dimethyl sulfide (CH 3 ) 2 S - cabbage like smell - dimethyl sulfate (CH 3 O) 2 SO 2 . - onion-like odour in high concn. VOSC - Volatile Organic Sulfur compound - normally effective at very low concn (ppb or less) Examples: 3-(methylthio)-1-propanol methanethiol dimethyl disulfide Dimethyl trisulfide Allyl methyl sufide

9. Management

10. Yeast growth late-phase or non-growth H2S growth Extended and throughout ferment Early stage growth (solid) Prolonged H2S growth (dotted)

12. H2S Production in the late phase of ferment - occurs after yeast growth has finished, late in ferment, usually when sugar levels are below 50-100g/L - varies among varieties: Syrah can be bad, Cab can be good - varies among yeast strains - typically unresponsive to DAP additions - can respond well to aeration or vitamins, though only lowered and not eliminated - it is thought that it this time the yeast cells are trying to get N for cell maintenance activities by mobilizing reserves of org. Sulphur compounds (previously mentioned methionine, cysteine and also glutathione). And again, breaking these down results in H 2 S production. - aeration isn't the simple answer as it can lead to the formation of disulfides if mercaptans are also present. (good winemakers have good palates) -disulfides are less volatile but can revert into mercaptans in the right 'conditions' Recent studies show that early H 2 S production ceased b/f midpoint of ferment it was not detected in final wine (cold settled, no copper addition)

14. Indigenous 'wild' and other yeasts Older literature and possibly a myth about indigenous yeasts causing more H2S production seems to be just that, a myth. That is, no more or less a factor than with most inoculated yeasts. That also applies to the idea that wild yeasts are the cause of H 2 S production in wild yeasts. There are only theories and no real literature on the role of non- Saccharomyces yeasts effects on the ferment. General knowledge is that these yeasts are in decline by the middle stages of the ferment it is not considered a factor, again relating to the time of the H 2 S production. More Yeast issues: Some yeasts are known to act as a source of mercaptans and related VSCs. Different results in different musts in relation to DAP additions. Once again, grape must and wine is a complex matrix. Some yeasts are known to cause issues in certain types of musts. More research is being done.

16. DAP addition - varies within yeast strain and conditions - can effect varietal characteristics such as 3MH and 3MHA in SB because synthesis only occurs in conditions when DAP might be needed - can only delay H2S production in some yeast strains and leave residual levels in the wine, because once again timing is crucial. So bumping up the YAN levels with DAP in low nutrient musts can actually hurt the wine in some cases. And this biochemical mechanism is unclear But more additions later on can help this out showing that different patterns of H2S production can result from DAP additions. - yeasts that respond well to DAP when the initial juice is YAN is about 250mg/L but just getting the YAN level up to that level is not that simple. Remember, DAP is only 20% N. So what's the rest?... And what if DAP or other N supplements fail to cease H2S productions or worse yet increase H2S production? First a test can be done but then...... The mgmt techniques....

17. Vitamins - vitamin deficiency can be directly related to H2S production (Henschke et al. ) - sound, healthy fruit is a key issue. Can relate to vineyard disease but also handling during harvest, transport, destemming/crushing, etc. - avoid microbial growth or wild yeast growth prior to inoculation to avoid depletion of thiamine, leading to sluggish ferments - use a careful balance of SO2 during transport and handling of fruit. - use of yeast vitamin preparations at or before inoculation along with a small amount of DAP can control H2S production

18. Commercial nutrients There are many of these on the market and many wineries use these during yeast inoculation (starter culture) and during the ferment. However, some of these do not contain sufficient amounts of Nitrogen to help with production of H2S. Copper additions - Copper sulphate can effectively remove H2S and mercaptans, it does not remove disuphides - Also can strip wine of varietal characters such as other important thiols For instance, the 3MH and 3MHA, which can be more important to the wine than the presence of some other unwanted thiols

19. Another 'underlying' issue is that of closure type We don't want the 'new cork taint' Though consumers may be more accepting of mercaptans in their wines, the rate of 2.2% of screwcapped wines is alarming. Clearly why AWRI has ongoing research trials.

20. References: Goode, J. Mercaptans and other volatile sulfur compounds in wine. Retrieved 26 July 2010 from http://www.wineanorak.com/mercaptansinwine.htm Jackson, R. Wine Science.3 rd Edition 2008. Theron, C. Wynboer Dec 2009 The practical management of Hydrogen Sulphide Moore, D. Various course handouts. Structural and Metabolic Biochemistry. Eastern Institute of Technology. Ugliano, M., Winter, G., Coulter, A.D., Henschke, P.A. The Australian & New Zealand Grapegrower and Winemaker. Annual Technical Issue 2009. Practical management of hydrogen sufide during fermentation – an update . Various resources on www.wikipedia.org Dobson, J., 2010. personal communications.