Lab amylase Presence and action of salivary amylase and the effect of pH Main previous concepts: Starch / Amylase / Iodine test Starch (or amylum) is a carbohydrate consisting of a large number of glucose units joined together. Amylase is an enzyme that breaks starch down into sugar. Amylase is present in human saliva, where it begins the chemical process of digestion. Amylase splits starch to glucose. The Iodine test (or lugol) is used to test for the presence of starch. Iodine solution reacts with starch producing a blue black color.

1. Presence and action
of salivary amylaseamylaseamylaseamylase
and the effect of pHpHpHpH
Main previous concepts: Starch / Amylase / Iodine test
Starch (or amylum) is a carbohydrate consisting of a large number of glucose
units joined together.
Amylase is an enzyme that breaks starch down into sugar. Amylase is present
in human saliva, where it begins the chemical process of digestion.
Amylase splits starch to glucose.
The Iodine test (or lugol) is used to test for the presence of starch.
Iodine solution reacts with starch producing a blue black color.
MATERIALSMATERIALSMATERIALSMATERIALS. (Drawing and name of materials)

2. 1 2 3 4
Water Bread
water
Bread
water
saliva
Bread
water
saliva
acid
Iodine test
(adding
Lugol)
Colour
Test result
+ or - ?
Presence of
starch?
RESULTSRESULTSRESULTSRESULTS
Explain the results for each tube.
http://www.biotopics.co.uk/nutrition/amylex.html
http://www.biologyjunction.com/amylase_on_starch_lab.htm
http://www.medbio.info/Horn/Sugars4Kids/flour_and_wood.htm