Soumettre la recherche
Mettre en ligne
Vector delivery
•
0 j'aime
•
364 vues
Z
zwiegers
Suivre
Signaler
Partager
Signaler
Partager
1 sur 45
Recommandé
Investigating viral vector targeting and future plans in gene therapy.
Discovering my research interest by eric garson sheffield university 2017
Discovering my research interest by eric garson sheffield university 2017
Eric Garson
DNA copy number variations (CNVs) play an important role in the pathogenesis and progression of cancer. While array comparative genomic hybridization (aCGH) has generally been used to identify CNVs in the whole genome, next-generation sequencing (NGS) provides an opportunity to characterize CNVs genome-wide with unprecedented resolution, even at the single cell level. However, CNV detection in single cells is faced with various challenges, such as incomplete genome coverage, introduction of sequence errors, GC bias and false positives. In this new poster, we show a method for capturing the entire genomic complexity of a single cell, overcoming these challenges and ensuring accurate detection of CNVs.
Enabling CNV Studies from Single Cells Using Whole Genome Amplification and L...
Enabling CNV Studies from Single Cells Using Whole Genome Amplification and L...
QIAGEN
SANGER SEQUENCING - 1st generation sequencing Sanger Sequencing Workflow: PCR amplification (target enrichment) PCR purification (primer, dNTPs) Sequencing reaction (bi-directional) Sequencing purification (primer, dNTPs, ddNTPs) Electrophoretic run on sequencer Sequencing lecture Alignment to reference SANGER SEQUENCING: LIMITATIONS Analytical sensitivity*: 99% PCR-Based no detection deletion/duplication rearrangements del/dup BRCA = 4-28% of all BRCA mutations in most population** Level of mosaicism > 20% Low throughput (82496 capillary tubes) Labor intensive Time consuming High cost (large size gene or more genes)
20160219 - S. De Toffol - Dal Sanger al NGS nello studio delle mutazioni BRCA