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nettle propsal presentation power point Afaf final copy.pptx

  1. ‫الرحيم‬ ‫الرحمن‬ ‫هللا‬ ‫بسم‬ Al-Quds University Faculty of Science & Technology Applied and Industrial Technology Program Extract of Stinging Nettle, Phase behavior and total bioactive compounds Prepared by :Afaf Abuhilal Registration No:2012450 Supervisor: Dr. Ibrahim Kayyali Co Supervisor: Dr. Fuad Al Rimawi
  2. Introduction • stinging nettle, (Urtica dioica), common nettle, weedy perennial plant of the nettle family (Urticaceae). • The stinging trichomes of the leaves and stems have bulbous tips that break off when brushed against, revealing needlelike tubes that pierce the skin. • It is a wild plant, In Palestine we find it in colonies, common ( ‫قريص‬ ) it is known for years in medicine and kitchen use.
  3. Nutritional composition: -Fresh leaves contain approximately: 90% moisture, up to 3.7% proteins, 0.6% fat, 2.1% ash, 6.4% dietary fiber and 7.1% carbohydrates . -sterols , fatty acids, polysaccharides, -minerals such as(iron ,potassium ,manganese , calcium.) -vitamin(A, B, C, D and K). -Protein and amino acids -Poly phenols (Ursolic acid and quercetine).
  4. Polyphenols of Urtica Phenolic compounds from U. dioica roots (9)
  5. Medical benefits of Urtica: Graphical abstract (8)
  6. Medical Benefits of Urtica • It inhibits Corona virus replication, a study was conducted on a mouse model. (10) • When Treated with UDA at 5 mg/kg significantly protected the mice against a lethal infection with mouse-adapted SARS-CoV (p<0.001. • The mechanism of Action of Urtica, it might bind to Nacetylglucosamine-like residues present on the glycosylated envelope glycoproteins, thereby preventing virus attachment to cells.
  7. Side effects and toxicity: • It is considered as a safe plant. • Internal use of Nettle might cause diarrhea , constipation, and upset stomach in some people. • When applied to the skin: Stinging nettle is possibly safe. Touching the stinging nettle plant can cause skin irritation. • Fresh nettle causes stings and can rarely lead to severe allergic reactions in susceptible individuals.
  8. Preparation of plant material: 2- dry the plant for 8-10 days 4- 150 ml ethanol 5% in soxhlet 8 hour 3- crushed into powder 5- evaporator using rotary evaporator 1- collected nettle plant (leaves, stalk & ( roots
  9. Methods of Extraction: Extraction: is the separation of medicinally active portions of plant or animal tissues from inactive or inert components by selective solvents in standard extraction procedures. -method of extraction: 1-Soxhlet Extraction. 2-Ultrasound-assisted Extraction (UAE) 3-Supercritical Fluid Extraction (SFE) 4-Shake extraction 5-Accelerated Solvent Extraction (ASE)
  10. Soxhlet Extraction: In this method ,the finely solid material containing desired compound is placed inside thimble made from thick filter paper,which is loaded into the main chamber of the extractor. The extracting solvent in flask is heated and its vapors condense . The condensed extractant drips into the thimble containing the crude drug and extract it by contact,when the level of liquid in chamber rises to the top of siphon tube ,the chamber automatically empty by siphon side arm into flask.
  11. Method for phenolic acid determination: For separation ,purification and identification of phenolic compound ,numerous chromatography method have been used ,such as: 1-High performance liquid chromatography (HPLC). • 2-Gas chromatography • 3-Capillary electrophoresis
  12. Total content of Phenols • Folin-Ciocalteuassay involves the reduction of the F–C reagent with phenolic compounds in an alkaline medium. The reaction is accompanied by the formation of a blue-colored complex that has a maximum absorbance at 765 nm. The absorbance is directly proportional to the TPC which is reported as Gallic acid equivalent. • Quantitation of total flavonoid content (TFC) is widely performed using the aluminum chloride colorimetric assay against a flavonoid standard assuming equal responses from all flavonoids
  13. Antimicrobial activity • The antimicrobial effects of the plant extracts will be evaluated using Antimicrobial tests which will be carried out by the Disc diffusion method. • This method is based on the diffusion of an antibiotic from a filter paper disc through the solidified culture medium (Muller- Hinton agar) of Petri dishes. • Whatman filter paper will be used to prepare small discs (6 mm in diameter), then sterilized at 121 C for 15 min in an autoclave. The discs were separately impregnated with the extracts for 1-4 hour at room temperature and allowed to dry. • A swap of bacterial broth will be dispersed on the agar plate surface. After drying, the previous discs placed on the Petri dishes and then incubated at 37 C for 24 to 48 hour. Three replicated plates used for each treatment. The diameter of inhibition zone created by each disc will be measured (in mm) using a ruler.
  14. Literature review: • (Kornelia Koszegi, etal, 2014)) applied three kinds of solvents into two type of extraction techniques : • Soxhelt extraction with 96%ethanol,Soxhelt extraction with n-hexane and supercritical fluid extraction (SFE)with CO2,by repeating the measurement and analysis three times, • The result exhibited that Soxhlet extraction with ethanol is the highest while the soxhlet extraction and the super critical extraction has lower yield ,the total polyphenol content and the H-donating ability of these extracts were low .
  15. Literature review • (Semih Otles and Buke, (2011)), different amount of nettle were collected from different region to study its phenolic component and antioxidant activities , nettle is seperated into 3 part:leaves,stalk and leaves. • To analysis them he used HPLC . • Folin –Ciocalteu method to collect total phenolic components ,and DPPH to measure antioxidat activity. • According to the result the component of each part had different quantity and quality for example phenolic content : • Root 7.82/stalk 9.91/leaves 7.62gm
  16. Literature review • ( Hanan Bander et al,(2013)),studied and applied different method and techniques with different solvent for extraction of bioactive compound from Nettle .(maceration,reflux,soxhlet,hydrodistillation,Ultrasound- Assisted Extraction (UAE)and Microwave-Assisted Extraction (MAE)). • Using different solvents (hexane,dichloro methane,aceton,ethanol,water). • The result show that the extraction method , solvent and time had a significant effect on the amount of extracted compound .Ethanol was the most effective organic solvent and soxhelt had the highest extraction yield.
  17. Literature review • Namazi et al,(2011),studied the effect of nettle extract on insulin sensetivity and some inflammatory indicators in type2 diabetic patient ,this was randomized double-blind clinical trial on 50men and woman with type2 diabetes done over an 8 week period . • They received 100mg nettle extract in3 portions a day for 8weeks.interleukin6 (IL-6)and Fasting insulin concentration were measured . • Insulin sensitivity was calculated at the beginning and the end of the study . • After8 weeks, IL-6 showed a significant decrease ,so this finding showed that nettle extraction has decreasing effect on IL-6 in patients with type2 diabetes after 8 week trial.
  18. Literature review • (Capucine Bourgeois et al(2016)),used HPLC method to determined ursolic acid and quercetin in nettle extract, and to evaluated the antioxidant and anti-aging activity of these extract. • The anti-aging potential involves the inhibition of enzyme activity such as elastase and collagenase, and these inhibitory effect due to ursolic acid and quercetin present in nettle extract . • The result also show the extraction parameter such as :extraction time,temperature,and ethanol percentage.
  19. Literature review ( Fedorovska Maryana Ivanivna et ,al,(2018)). Used nettle fresh upper parts of stem with 5-6 leaves to developed gel with nettle juice and study its effect in rats . The result show that increase in the growth of rat wool ,increase in the number of the mast cell and increase in the hair follicle .
  20. Construction of phase diagram: -The pseudo ternary phase diagram consisting of oil, water, surfactant and co-surfactant mixture of different HLB values were constructed using water water titration method . 1-The sample of extracted oil and nonionic surfactant are insert into 10ml glass test tube with screw caps at room temperature at different weight ratios.
  21. Construction of phase diagram 2-mechenical shaking for 1min 3-titration with water is injected into each test tube (drop by drop). 4-vigorous mechanical shaking for 5min ,on the vortex due to the high viscosity of the surfactant and oil in order to guarantee homogenous dispersion . 5-placing the tube within thermostatic water bath to control the temperature. 6- To reach equilibrium left rest for 24hrs then the addition water and analyzing the samples.
  22. Construction of phase diagram 9:1 8:2 7:3 6:4 5:5 4:6 3:7 2:8 1:9 Weight ratio 0:9 0:8 0:7 0:6 0:5 0:4 0:3 0:2 0:1 Surfactant 1:0 2:0 3:0 4:0 5:0 6:0 7:0 8:0 9:0 Oil
  23. Determination of phase behavior : 1-Visual observation: microemulsion will identify by color, texture and transparency. 2- Anisotropy :is detected by the cross polarizer.clear isotropic,one-phase system were designated as microemulsion system .
  24. Time table: July 2023 May 2023 March- APR 2023 Feb 2023 Dec-Jan 2022 Nov 2022 X X 1- Literature Review X 2-Collecting and preparing sample X X X 3-Extraction X 4- writing X 5-Presentation of results
  25. References: 1-Ilhami Gulcin , O. Irfan Kufrevioglu , Munir Oktay ,Mehmet Emin Buyukokuroglu, Antioxidant, Antimicrobial,antiulcer and analgesic activities of nettle ,Journal of Ethnopharmacology (2004) 205-2015. Kornelia koszegi, Gyula Vatia , Erika Bekassy-Molnar, Comparison the Soxhlet and Supercritical Fluid Extraction of Nettle Root, Periodica polytechnica Chemical Engineering, (2015) 168-173. 3-Solvita Zeipina,InaAlsina,Liga Lipese and Mara Duma . Antioxidant Activity in Nettle and Garden Orache (Atriplex Hortensis L) Leaves During Vegetation Period .Chemine Technologija (2015) 28-33. 4-Semih Otles and Buket Yalcin, Phenolic Compound Analysis of Root,Stalk and Leaves of Nettle.The scientific World Journal, 12pages (2012). 5-Sadegh Fattahi ,Ebrahim Zabih,Zeinab Abedian, Roghayeh Pourbagher ,Ali Motevalizadeh Ardekani, Amrollah Mostafazadeh and Haleh Akhavan-Niaki.Total Phenolic and Flavonoid Content of Aqueous Extract of Stinging Nettle and in Vitro Antiproliferative Effect on Hela and BT-474 Cell Lines.Int Jmol Med Spring (2014) 1-
  26. References 6-Capucin Bourgeois, Emilie A.Leclerc ,Cyrielle Corbin, Joel Doussot,Valerie Serrano, Jean-Raymond Vanier,Daniel Auguin, Eric Lain and Christoph Hano. Nettle as asource of antioxidant and anti-aging phytochemicals for cosmetic application ,Comptes Rendus Chemie(2016)1090-1100. 7-Lenka Drostinova, Jana Branisa, Dominika Boncikova and Klaudia Jomova. Effect of Drying Methods on Content of some Natural Pigments in Urtica DioicaL. An Melissa Officinalis L. Journal of Microbiology, Biotechnology and Food SciencemScience,(2015)182-185. 8-Stinging Nettle (Urtica dioica L.): Nutritional Composition, Bioactive Compounds, and Food Functional Properties by Hari Prasad Devkota, Molecules 2022, 27(16), 5219 9-Therapeutic Perspectives of Molecules from Urtica dioica Extracts for Cancer treatment by Sabrina Esposito Molecules 2019,24 (15), 2753 10- Yohichi Kumakia, Miles K. Wanderseea, Aaron J. Smitha, Yanchen Zhoub.Inhibition of severe acute respiratory syndrome coronavirus replication in a lethal SARS-CoV BALB/c mouse model by stinging nettle lectin, Urtica dioica agglutinin.J.Antiviral (2011) 90(1): 22–32.

Notes de l'éditeur

  1. Genus : Urtica, FAMILY: Urticaceae … stinging hair
  2. Higher that other wild plants