SlideShare une entreprise Scribd logo
1  sur  2
Télécharger pour lire hors ligne
Alexandra Papadopoulou | Molecular analysis of HRAS and KRAS genes in thyroid cancer in the Greek population
1
UNIVERSITY OF THESSALY
FACULTY OF HEALTH SCIENCES
DEPARTMENT OF BIOCHEMISTRYAND BIOTECHNOLOGY
MASTER OF SCIENCE: “MOLECULAR NIOLOGY AND GENETICS
APPLICATIONS - DIAGNOSTIC MARKERS”
MASTER THESIS
MOLECULAR ANALYSIS OF HRAS AND KRAS GENES IN
THYROID CANCER IN THE GREEK POPULATION
ALEXANDRA PAPADOPOULOU
ABSTRACT
Thyroid cancer is the most common type of endocrine malignancy and its frequency
is increasing steadily over the last decades. Based on histopathological characteristics,
thyroid tumors are classified into four major types (papillary, follicular, medullary
and anaplastic), while additional variants exist between these major types, with the
most common being the follicular subtype of papillary carcinoma. Previous studies
have shown that certain signaling pathways play a fundamental role in the
pathogenesis of thyroid cancer, and thus can be considered as potential targets for
therapy. For example, activation of the MAPK signaling pathway, due to genetic
mutations, is commonly found in different types of thyroid cancer. These include
point mutations of the BRAF gene (BRAFV600E
), point mutations of the RAS (HRAS,
KRAS and NRAS) genes (codons 12, 13 and 61) as well as RET / PTC and PAX8 /
PPARγ genetic rearrangements. It should be noted that the above mutations are
reported to be mutually exclusive. Limited data are available on the frequency of
these mutations in the Greek population. The purpose of this study was to investigate
the correlation of point mutations of the KRAS gene (codons 12, 13, 61) and the
HRAS gene (codon 61) with different types of thyroid cancer in the Greek population.
The study involved 33 patients with differentiated thyroid cancer, including 11
papillary, 14 follicular variants of papillary carcinoma, 7 follicular and 2 medullary
carcinomas. The experimental procedure included the isolation of genomic DNA from
paraffin-embedded tissue biopsies, the amplification of specific regions of the RAS
genes using Polymerase Chain Reaction (PCR), and the direct sequencing of the
Alexandra Papadopoulou | Molecular analysis of HRAS and KRAS genes in thyroid cancer in the Greek population
2
purified PCR products. The results of the study did not identify any mutation at
codons 12, 13 and 61 of the KRAS gene and at codon 61 of the HRAS gene in all the
tested samples. However, in order to be able to draw firm conclusions, this study must
be extended to include additional mutations of the RAS and the BRAF genes, as well
as genetic rearrangements, in a larger sample population.

Contenu connexe

Tendances

Disruption of focal adhesion kinase and p53 interaction with small molecule c...
Disruption of focal adhesion kinase and p53 interaction with small molecule c...Disruption of focal adhesion kinase and p53 interaction with small molecule c...
Disruption of focal adhesion kinase and p53 interaction with small molecule c...Enrique Moreno Gonzalez
 
MONOGENIC AND POLYGENIC DISEASES
MONOGENIC AND POLYGENIC DISEASESMONOGENIC AND POLYGENIC DISEASES
MONOGENIC AND POLYGENIC DISEASESJuliana Vargas
 
Cancer therapy at gene level
Cancer therapy at gene levelCancer therapy at gene level
Cancer therapy at gene levelSuganyaPaulraj
 
Comparative analysis of gene expression reulation in mouse rat and human Spri...
Comparative analysis of gene expression reulation in mouse rat and human Spri...Comparative analysis of gene expression reulation in mouse rat and human Spri...
Comparative analysis of gene expression reulation in mouse rat and human Spri...constantina mylona
 
Plegable pp1
Plegable pp1Plegable pp1
Plegable pp1MafeU
 
Plegable pp1
Plegable pp1Plegable pp1
Plegable pp1MafeU
 
DNA structure, chromosomes and reparation
DNA structure, chromosomes and reparationDNA structure, chromosomes and reparation
DNA structure, chromosomes and reparationTatiana Gomez Gonzales
 
The opportunity of stem cell to treat diabetes and cancer
The opportunity of stem cell to treat diabetes and cancerThe opportunity of stem cell to treat diabetes and cancer
The opportunity of stem cell to treat diabetes and cancerResearchsio
 
A 3′-untranslated region KRAS variant and triple-negative breast cancer: a ca...
A 3′-untranslated region KRAS variant and triple-negative breast cancer: a ca...A 3′-untranslated region KRAS variant and triple-negative breast cancer: a ca...
A 3′-untranslated region KRAS variant and triple-negative breast cancer: a ca...UCLA
 
Dna repair mechanism
Dna repair mechanismDna repair mechanism
Dna repair mechanismMabel Tabares
 
Cellular level cancer therapy
Cellular level  cancer therapyCellular level  cancer therapy
Cellular level cancer therapySuganyaPaulraj
 
Trabajo plegable biomol
Trabajo plegable biomol Trabajo plegable biomol
Trabajo plegable biomol juansebastianpz
 
Open Source Pharma /Genomics and clinical practice / Prof Hosur
Open Source Pharma /Genomics and clinical practice / Prof Hosur Open Source Pharma /Genomics and clinical practice / Prof Hosur
Open Source Pharma /Genomics and clinical practice / Prof Hosur opensourcepharmafound
 
RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...
RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...
RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...Alejandro Garzón
 
RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...
RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...
RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...Alejandro Garzón
 

Tendances (19)

Disruption of focal adhesion kinase and p53 interaction with small molecule c...
Disruption of focal adhesion kinase and p53 interaction with small molecule c...Disruption of focal adhesion kinase and p53 interaction with small molecule c...
Disruption of focal adhesion kinase and p53 interaction with small molecule c...
 
MONOGENIC AND POLYGENIC DISEASES
MONOGENIC AND POLYGENIC DISEASESMONOGENIC AND POLYGENIC DISEASES
MONOGENIC AND POLYGENIC DISEASES
 
Cancer therapy at gene level
Cancer therapy at gene levelCancer therapy at gene level
Cancer therapy at gene level
 
Seminario biomol final
Seminario biomol finalSeminario biomol final
Seminario biomol final
 
Single cell pcr
Single cell pcrSingle cell pcr
Single cell pcr
 
Comparative analysis of gene expression reulation in mouse rat and human Spri...
Comparative analysis of gene expression reulation in mouse rat and human Spri...Comparative analysis of gene expression reulation in mouse rat and human Spri...
Comparative analysis of gene expression reulation in mouse rat and human Spri...
 
Plegable pp1
Plegable pp1Plegable pp1
Plegable pp1
 
Plegable pp1
Plegable pp1Plegable pp1
Plegable pp1
 
DNA structure, chromosomes and reparation
DNA structure, chromosomes and reparationDNA structure, chromosomes and reparation
DNA structure, chromosomes and reparation
 
Monogenic Diseases
Monogenic Diseases Monogenic Diseases
Monogenic Diseases
 
The opportunity of stem cell to treat diabetes and cancer
The opportunity of stem cell to treat diabetes and cancerThe opportunity of stem cell to treat diabetes and cancer
The opportunity of stem cell to treat diabetes and cancer
 
A 3′-untranslated region KRAS variant and triple-negative breast cancer: a ca...
A 3′-untranslated region KRAS variant and triple-negative breast cancer: a ca...A 3′-untranslated region KRAS variant and triple-negative breast cancer: a ca...
A 3′-untranslated region KRAS variant and triple-negative breast cancer: a ca...
 
Dna repair mechanism
Dna repair mechanismDna repair mechanism
Dna repair mechanism
 
Cellular level cancer therapy
Cellular level  cancer therapyCellular level  cancer therapy
Cellular level cancer therapy
 
Trabajo plegable biomol
Trabajo plegable biomol Trabajo plegable biomol
Trabajo plegable biomol
 
Genetic disesase
Genetic disesaseGenetic disesase
Genetic disesase
 
Open Source Pharma /Genomics and clinical practice / Prof Hosur
Open Source Pharma /Genomics and clinical practice / Prof Hosur Open Source Pharma /Genomics and clinical practice / Prof Hosur
Open Source Pharma /Genomics and clinical practice / Prof Hosur
 
RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...
RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...
RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...
 
RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...
RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...
RESEARCHERS MAP SIGNIFICANT OF FUNCTIONAL SEQUENCES OF MOUSE GENOME AND ACTIV...
 

Similaire à Molecular analysis of HRAS and KRAS genes in thyroid cancer in the Greek population

A KRAS-variant is a Biomarker of Poor Outcome, Platinum Chemotherapy Resistan...
A KRAS-variant is a Biomarker of Poor Outcome, Platinum Chemotherapy Resistan...A KRAS-variant is a Biomarker of Poor Outcome, Platinum Chemotherapy Resistan...
A KRAS-variant is a Biomarker of Poor Outcome, Platinum Chemotherapy Resistan...UCLA
 
9979-152032-3-PB (2)
9979-152032-3-PB (2)9979-152032-3-PB (2)
9979-152032-3-PB (2)Andrew Hinton
 
Poster_Molecular analysis of BRAF and RAS family genes in thyroid carcinoma i...
Poster_Molecular analysis of BRAF and RAS family genes in thyroid carcinoma i...Poster_Molecular analysis of BRAF and RAS family genes in thyroid carcinoma i...
Poster_Molecular analysis of BRAF and RAS family genes in thyroid carcinoma i...Alexandra Papadopoulou
 
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...JohnJulie1
 
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...AnonIshanvi
 
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...daranisaha
 
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...semualkaira
 
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...semualkaira
 
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...EditorSara
 
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...NainaAnon
 
Clinical Cancer Research Publication
Clinical Cancer Research PublicationClinical Cancer Research Publication
Clinical Cancer Research PublicationDavid W. Salzman
 
Cancer Res-2015-Bonastre-1287-97
Cancer Res-2015-Bonastre-1287-97Cancer Res-2015-Bonastre-1287-97
Cancer Res-2015-Bonastre-1287-97Sara Verdura
 
Collision Tumour-IRB -Final.pptx
Collision Tumour-IRB -Final.pptxCollision Tumour-IRB -Final.pptx
Collision Tumour-IRB -Final.pptxsherin110346
 
全人關懷獎的簡報
全人關懷獎的簡報全人關懷獎的簡報
全人關懷獎的簡報bgbgbg
 
Seminario de bilogia molecular copia
Seminario de bilogia molecular   copiaSeminario de bilogia molecular   copia
Seminario de bilogia molecular copialaurisdurango07
 

Similaire à Molecular analysis of HRAS and KRAS genes in thyroid cancer in the Greek population (20)

A KRAS-variant is a Biomarker of Poor Outcome, Platinum Chemotherapy Resistan...
A KRAS-variant is a Biomarker of Poor Outcome, Platinum Chemotherapy Resistan...A KRAS-variant is a Biomarker of Poor Outcome, Platinum Chemotherapy Resistan...
A KRAS-variant is a Biomarker of Poor Outcome, Platinum Chemotherapy Resistan...
 
9979-152032-3-PB (2)
9979-152032-3-PB (2)9979-152032-3-PB (2)
9979-152032-3-PB (2)
 
Poster_Molecular analysis of BRAF and RAS family genes in thyroid carcinoma i...
Poster_Molecular analysis of BRAF and RAS family genes in thyroid carcinoma i...Poster_Molecular analysis of BRAF and RAS family genes in thyroid carcinoma i...
Poster_Molecular analysis of BRAF and RAS family genes in thyroid carcinoma i...
 
Cadher
CadherCadher
Cadher
 
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
 
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
 
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
 
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
 
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
 
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
 
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
Deadenylase Expression in Small Cell Lung Cancer Related To Clinical Characte...
 
Clinical Cancer Research Publication
Clinical Cancer Research PublicationClinical Cancer Research Publication
Clinical Cancer Research Publication
 
Annals of Mutagenesis
Annals of Mutagenesis Annals of Mutagenesis
Annals of Mutagenesis
 
Genome wide study
Genome wide studyGenome wide study
Genome wide study
 
Cancer Res-2015-Bonastre-1287-97
Cancer Res-2015-Bonastre-1287-97Cancer Res-2015-Bonastre-1287-97
Cancer Res-2015-Bonastre-1287-97
 
Collision Tumour-IRB -Final.pptx
Collision Tumour-IRB -Final.pptxCollision Tumour-IRB -Final.pptx
Collision Tumour-IRB -Final.pptx
 
MCR_Article_JW
MCR_Article_JWMCR_Article_JW
MCR_Article_JW
 
LYS.pdf
LYS.pdfLYS.pdf
LYS.pdf
 
全人關懷獎的簡報
全人關懷獎的簡報全人關懷獎的簡報
全人關懷獎的簡報
 
Seminario de bilogia molecular copia
Seminario de bilogia molecular   copiaSeminario de bilogia molecular   copia
Seminario de bilogia molecular copia
 

Molecular analysis of HRAS and KRAS genes in thyroid cancer in the Greek population

  • 1. Alexandra Papadopoulou | Molecular analysis of HRAS and KRAS genes in thyroid cancer in the Greek population 1 UNIVERSITY OF THESSALY FACULTY OF HEALTH SCIENCES DEPARTMENT OF BIOCHEMISTRYAND BIOTECHNOLOGY MASTER OF SCIENCE: “MOLECULAR NIOLOGY AND GENETICS APPLICATIONS - DIAGNOSTIC MARKERS” MASTER THESIS MOLECULAR ANALYSIS OF HRAS AND KRAS GENES IN THYROID CANCER IN THE GREEK POPULATION ALEXANDRA PAPADOPOULOU ABSTRACT Thyroid cancer is the most common type of endocrine malignancy and its frequency is increasing steadily over the last decades. Based on histopathological characteristics, thyroid tumors are classified into four major types (papillary, follicular, medullary and anaplastic), while additional variants exist between these major types, with the most common being the follicular subtype of papillary carcinoma. Previous studies have shown that certain signaling pathways play a fundamental role in the pathogenesis of thyroid cancer, and thus can be considered as potential targets for therapy. For example, activation of the MAPK signaling pathway, due to genetic mutations, is commonly found in different types of thyroid cancer. These include point mutations of the BRAF gene (BRAFV600E ), point mutations of the RAS (HRAS, KRAS and NRAS) genes (codons 12, 13 and 61) as well as RET / PTC and PAX8 / PPARγ genetic rearrangements. It should be noted that the above mutations are reported to be mutually exclusive. Limited data are available on the frequency of these mutations in the Greek population. The purpose of this study was to investigate the correlation of point mutations of the KRAS gene (codons 12, 13, 61) and the HRAS gene (codon 61) with different types of thyroid cancer in the Greek population. The study involved 33 patients with differentiated thyroid cancer, including 11 papillary, 14 follicular variants of papillary carcinoma, 7 follicular and 2 medullary carcinomas. The experimental procedure included the isolation of genomic DNA from paraffin-embedded tissue biopsies, the amplification of specific regions of the RAS genes using Polymerase Chain Reaction (PCR), and the direct sequencing of the
  • 2. Alexandra Papadopoulou | Molecular analysis of HRAS and KRAS genes in thyroid cancer in the Greek population 2 purified PCR products. The results of the study did not identify any mutation at codons 12, 13 and 61 of the KRAS gene and at codon 61 of the HRAS gene in all the tested samples. However, in order to be able to draw firm conclusions, this study must be extended to include additional mutations of the RAS and the BRAF genes, as well as genetic rearrangements, in a larger sample population.