1. BOROŹYME TECHNOLOGiES
Presents
BOROŹYME ¦ R ‘DZ-1X’
Concept Presentation on :
Enzyme Based Soil Stabilization
Nucleation Center Marketed by Large-Scale Production

2. INTRODUCTION.
 BORON INNOVATIONS PVT. LTD., Bhavnagar is an identity
created by panel of high qualified specialized scientists,
whose aim is to protect and deliver the environment, with
Nucleation Center at CHARUSAT University & Large - Scale
production at DHARA BIOTECH, innovative Enzymes
Technology has been developed for disposal of sewage
wastes, making earthen roads and constructing low cost
earthen mud houses.
 Company’s Vision is to make this technology reach ‘the
common people’. Go Green With
Bio-Enzymes

3. ENZYME BASED SOIL STABILIZATION?
 A process of using multi-enzyme
based formulation developed as an
effective aid to the workability,
mixibility, binding and compaction of
SOIL
Go Green With
Bio-Enzymes

4. WHERE CAN ENZYMES BE USED IN CONSTRUCTION?
 Making ROADS
 DAMS
Constructing earthen
 Making of Bricks
 MANY OTHER RELATED PROJECTS
Go Green With
Bio-Enzymes

5. WHAT IS “BOROŹYME¦R – DZ-1X” ?
IT IS THE FIRST GENERATION
FORMULATION OF SOIL
STABILIZATION ENZYMES FROM
DHARA BIOTECH
Go Green With
Bio-Enzymes

6. WHAT ARE THE BENEFITS OF USING
BOROŹYME¦R – DZ-1X?
A. Reduced construction costs
B. Longer useful lives
C. Minimal road maintenance costs
D. Better wear qualities
E. Increased transport capacity
F. Greater passenger comfort
G. Lower vehicle operating costs
H. Reduced traffic accidents
I. Excellent environmentally safety
Go Green With
Bio-Enzymes

7. WHAT ARE THE APPLICATIONS OF USING
BOROŹYME¦R – DZ-1X?
A. Load Support - SBC
B. Stabilized Base for Asphalt & Concrete
C. Slope Protection
D. Channel Protection
E. Earth Retention
F. Erosion Control
G. Dusting Control
H. Soil Sealing
I. Roadways Shoulder
J. Surface Flow Protection
Go Green With
Bio-Enzymes

8. PROPERTIES:
A. Increased compressive strength
B. Reduced compaction effort and improved soil
workability
C. Increased soil density
D. Lowered water permeability
Go Green With
Bio-Enzymes

9. ADVANTAGES:
A. Environmentally safe
B. Cost effective - Simple to use
C. Maximum Use of Locally available materials.
D. Reduces the swelling capacity
E. Increase in CBR, MDD, SBC.
Go Green With
Bio-Enzymes

10. ‘BOROŹYME¦R – DZ-1X’ DELIVERS:
For making sub-base for paved/unpaved roads with
‘BOROŹYME¦R – DZ-1X’:
1. IT IMPROVES THE SOIL STRUCTURE
SUFFICIENTLY TO ACHIEVE CONFIRMED
SAVINGS OF GREATER THAN 25%
Go Green With
Bio-Enzymes

11. ‘BOROŹYME¦R – DZ-1X’ DELIVERS:
For making sub-base for paved/unpaved roads with
‘BOROŹYME¦R – DZ-1X’:
2. STRENGHTENS SUB-BASE LAYER
IN PREPARATION FOR CHIP-SEAL
OPERATIONS
Go Green With
Bio-Enzymes

12. ‘BOROŹYME¦R – DZ-1X’ DELIVERS:
For making sub-base for paved/unpaved roads with
‘BOROŹYME¦R – DZ-1X’:
3. INCREASES THE CBR OF SUB-BASE TO
SUPPORT HEAVY TRAFFIC LOADS AT
LOWER CRUST THICKNESS.
Go Green With
Bio-Enzymes

13. BOROŹYME¦R DELIVERS:
For making sub-base for paved/unpaved roads with
BOROŹYME¦R:
4. REDUCES SUCH COMMON ROAD
SURFACE PROBLEMS AS CRACKING AND
SURFACE LOOSENING
Go Green With
Bio-Enzymes

14. BOROŹYME¦R DELIVERS:
For making sub-base for paved/unpaved roads with
BOROŹYME¦R:
5. REDUCES THE COST OF PURCHASING AND
TRANSPORTING CONSTRUCTION MATERIALS
FOR ROAD RECONDITIONING BY REUSING IN-
SITU MATERIALS AND IMPROVING POORER
Go Green With
QUALITY LOCAL SOILS
Bio-Enzymes

15. BOROŹYME¦R DELIVERS:
For making sub-base for paved/unpaved roads with
BOROŹYME¦R:
6. EXTENDSTHE USEFUL LIFE OF
ASPHALT AND CONCRETE ROAD
SURFACES AND STABILIZES ROAD
SHOULDERS
Go Green With
Bio-Enzymes

16. BOROŹYME¦R DELIVERS:
For making sub-base for paved/unpaved roads with
BOROŹYME¦R:
7. DUE TO THE HIGH LEVELS OF COMPACTION
ACHIEVED, IT HELPS IN PREVENTING
UPWARD CAPILLARY MOVEMENT OF WATER
FROM BELOW THE SUB-BASE, THEREBY
PROTECTING THE ROADS FROM COLLAPSING
DUE TO WATER STRESSES.
Go Green With
Bio-Enzymes

17. Expected changes in soil CBR with the treatment of BoroŹyme¦R DZ-1X:
Plasticity Corresponding Expected Expected Expected Plasticity Corresponding Expected Expected Expected
index of % CBR of soil range of new % CBR ranged of index of % CBR of soil range of new % CBR ranged of
soil variation after DZ-1X variation in soil variation after DZ-1X variation in
in % CBR Treatment % CBR of soil in % CBR Treatment % CBR of soil
of soil (±) after DZ-1X of soil (±) after DZ-1X
treatment (+) treatment (+)
0 100 100 24 5.0 2 12.1 4
5 39.3 40.0 25 5.1 2 12.8 4
10 18.5 2 38.0 5 26 4.7 2 10.7 4
11 16.0 3 34.7 5 27 4.5 2 10.0 4
12 14.0 2 31.7 5 28 4.4 2 9.5 4
13 12.3 2 29.0 5 29 4.3 2 8.9 4
30 4.3 2 8.5 4
14 10.8 2 39.3 5
31 4.2 2 8.1 4
15 7.7 2 19.3 5
32 4.2 2 7.7 4
16 8.7 2 22.3 5
33 4.1 2 7.4 4
17 7.9 2 20.6 5
34 4.1 2 7.0 3
18 7.2 2 19.0 5 35 3.5 2 8.8 3
19 6.6 2 17.5 5 36 4.0 2 6.5 3
20 6.2 2 16.2 5 37 4.0 2 6.3 3
21 5.8 2 15.0 4 38 4.0 2 6.1 3
22 5.5 2 14.0 4 39 4.0 2 5.9 3
23 5.2 2 13.0 4 40 0.9 2 2.1 3

18. Graphical Representation:-
100
90
80
70
60
50
40
30
20
10
0
0 5 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40
Corresponding % CBR of soil Expected new % CBR after DZ-1X Treatment
Power (Corresponding % CBR of soil) Power (Expected new % CBR after DZ-1X Treatment)

19. Financial Savings..
Quantity of DZ-1X required per square meter of constructed base
CVPD (30MT)
All values are
All Values are in
in mm depth liters required CVPD (30MT)
required 0-15 15-45 45-150 150-450 CBR 0-15 15-45 45-150 150-450
2 275 365 370 455 2 0.06 0.08 0.08 0.10
3 200 265 330 320 3 0.04 0.06 0.07 0.07
4 125 200 260 315 4 0.03 0.04 0.06 0.07
5 100 165 210 260 5 0.02 0.04 0.05 0.06
6 60 115 150 175 6 0.01 0.03 0.03 0.04
7 60 115 150 175 7 0.01 0.03 0.03 0.04
Corresponding 10 30 70 85 125 corresponding 10 0.01 0.02 0.02 0.03
CBR of base 15 30 50 75 CBR of base 15 0.00 0.01 0.01 0.02
soil 20 30 50 soil 20 0.00 0.00 0.01 0.01

20. Cost of BoroŹyme¦R ‘DZ-1X’ required per square meter of
constructed base
CVPD (30MT)
0-15 15-45 45-150 150-450
2 60.00 80.00 80.00 100.00
3 40.00 60.00 70.00 70.00
4 30.00 40.00 60.00 70.00
5 20.00 40.00 50.00 60.00
6 10.00 30.00 30.00 40.00
7 10.00 30.00 30.00 40.00
10 10.00 20.00 20.00 30.00
Cost : Rs.1000/liter
corresponding CBR of 15 0.00 10.00 10.00 20.00
base soil 20 0.00 0.00 10.00 10.00

21. Taking an example:-
CBR value 2, CVPD of 15-45 vehicles:
 Parameters used:
 100 meter of road
 6 meter width of road
 Base depth of 365mm (0.365 mts)
 Cost of enzyme: Rs.1000 per liter
 Soil is used from on site, and a mixture of 20% metal stones are
used.
Go Green With
Bio-Enzymes

22.  THEN,
 Conventional cost is estimated to be at: Rs. 345.99 per sq.
meter
 Enzyme based cost is estimated to be at: Rs. 186.92 per sq.
meter
 SAVING (IN INR): Rs. 159.07 per sq. meter
 SAVINGS (IN %): 45.975% per sq. meter
Go Green With
Bio-Enzymes

23. Environmental benefits:
 Minimizes material loss of surface gravel on soil roadways due to
erosion or abrasion by traffic.
 Reduces the ongoing cost and environmental impact of the
purchase, transport and spreading of replacement gravel.
 Minimizes the harmful production and use of crushed rock and
historical mineral stabilizers in road construction and maintenance.
 Reduces fuel usage associated with frequent, short interval road
repairs.
 Lessens the impact of open gravel mines and pits.
BoroŹyme¦R DZ-1X allows maximum leveraging of existing sources.
Go Green With
Bio-Enzymes

24. BoroŹyme¦R Delivers
Stronger, Longer Lasting Roads
At A Lower Cost Than
Conventional Road Designs
Go Green With
Bio-Enzymes

25. MAKING SUB-BASE FOR ROAD CONSTRUCTION
Go Green With
Bio-Enzymes

26. Equipment Required.
Go Green With
Bio-Enzymes

27. STEPS INVOLVED IN MAKING A QUALITYENZYME
STABILIZED ROAD
IDENTIFY SOIL
EXCAVATE/GRADING
ENZYME CHARGING
LEVELING AND CONSOLIDATION
CURING
Go Green With
Bio-Enzymes

28. The activities to be undertaken while
applying Soil Stabilization Enzymes:
1. Grading and side drain construction
2. Ripping the road surface
3. Pulverizing the broken soil
4. Formulation spraying
5. Rotovating
6. Compacting
Go Green With
Bio-Enzymes

29. 1.) IDENTIFYING THE SOIL.
1(a). Identify The Soil Type
1(b). Determination Of The
Optimal Moisture Content
Required To Attain
Best Compaction
Go Green With
Bio-Enzymes

30. 2.) EXCAVATION/GRADING…
Excavation/
Grading to
required
depth or
application
of soil raised
to required
height
Go Green With
Bio-Enzymes

31. …EXCAVATION/GRADING…
Go Green With
Bio-Enzymes

32. …EXCAVATION/GRADING.
Go Green With
Bio-Enzymes

33. 3.) ENZYME CHARGING…
BOROŹYME¦R
– ‘DZ-1X’ is mixed
with necessary
amount of water
to attain OMC
Go Green With
Bio-Enzymes

34. …ENZYME CHARGING…
BOROŹYME¦R –
‘DZ-1X’ solution
is applied to the
soil at the
site
Go Green With
Bio-Enzymes

35. …ENZYME CHARGING…
Go Green With
Bio-Enzymes

36. … ENZYME CHARGING…
BOROŹYME¦R
– ‘DZ-1X’
solution is
thoroughly
mixed into the
soil
Go Green With
Bio-Enzymes

37. …ENZYME CHARGING.
REMEMBER:
THROUGHLY MIXED THE ENZYME IS
LEFT FOR 24 HOURS TO ACTIVATE
THE SOIL
Go Green With
Bio-Enzymes

38. 4.) LEVELING AND CONSOLIDATION…
Go Green With
Bio-Enzymes

39. …LEVELING AND CONSOLIDATION…
Go Green With
Bio-Enzymes

40. …LEVELING AND CONSOLIDATION…
Go Green With
Bio-Enzymes

41. …LEVELING AND CONSOLIDATION.
Go Green With
HINT: Good construction practices
Bio-Enzymes
give longer lasting surfaces

42. 5.) CURING.
A 24 hours
curing periods
allows for good
cohesion
between the soil
particles
Go Green With
Bio-Enzymes

43. 5.) CURING

44. POST CURING
 If the surface is to be paved with either Bitumen
or concrete, a surface drying of 3-4 days is
advisable
 If surface is not to be sealed, i.e. rural roads
(unpaved) is to be made, some gravel fines are
to be added to the surface to aid vehicles gain
traction in wet conditions.
Go Green With
Bio-Enzymes

45. FINISHED SURFACES
Go Green With
Bio-Enzymes

46. FINISHED SURFACE
Go Green With
Bio-Enzymes

47. FINISHED SURFACE
Go Green With
Bio-Enzymes

48. FINISHED SURFACE
Go Green With
Bio-Enzymes

49. FINISHED SURFACES
Go Green With
Bio-Enzymes

50. Tar Finished Road

51. B O R O Ź Y M E ¦ R ‘DZ-1X’
(Recommendation of tests for earthen roads to be conducted after ‘DZ-
1X’ application):
Step 1. Before road construction: Step 3. Post construction:
A. Average plasticity index of soil: helps determine H. Approximately 24 hours later – MDD analysis by
moisture content needed to attain “maximum dry sand replacement method or BS Core Cutter
density” (MDD). Method.
B. Optimal moisture content (OMC) to reach MDD. I. CBR of soil – after 7 days, field analysis by
C. Laboratory MDD of soil. Dynamic Cone Penetration, lab analysis of
undisturbed sample (as per IS-2720).
D. Laboratory CBR. J. CBR of soil – after 30 days, field analysis by
E. If required: enzyme modified MDD of soil and PI. Dynamic Cone Penetration, lab analysis of
undisturbed sample (as per IS-2720).
Step 2. At time of construction:
Note: Light traffic is to be allowed after 3
F. Average moisture of soil on site, needed to offset hours of compaction, heavy (loaded) vehicles
the quantity of water that will need to reach OMC. to be allowed after 48 hours. If there is a
G. Field soil density of soil – helps determine quantity moisture problem, allow to evaporate then
soil required to finish compacted road. allow vehicles.
Go Green With
Bio-Enzymes

52. Application Notes:- Day 1:
1. Plan project with supervisory personnel. 9. Road barriers should be assembled and
2. Assemble all required Equipment. checked.
3. Make equipment maintenance checks. 10. Traffic diversion plan should be finalized.
4. Verify fuel supply. 11. Traffic control personnel should be
instructed according to the work plan.
5. Verify and make arrangements for water
and water supply. 12. Instructions should be given in case of
rain.
6. Verify BOROŹYME¦R ‘DZ-1X’ supply.
13. Security for equipment should be
7. Meet with operator personnel and discuss arranged.
procedure.
14. Contingency plans should be made in case
8. If new soil is required, it should be brought of equipment break down or problems.
in and put in place. Go Green With
Bio-Enzymes

53. Application Notes:- Day 2:
1. Mix BOROŹYME¦R ‘DZ-1X’ with water in water truck
2. Break up soil with rippers on grader.
3. Remove or crush large BOROŹYME¦R ‘DZ-1X’ in Water.
4. Cut shoulders with grader on each side of road first.
5. Mix soil with grader and (add aggregate if necessary).
6. Moisten soil with water truck spray bar.
7. Mix soil with grader blade from each side to the other.
8. Repeat #5 – #6 all day.
9. Wind row with grader blade on each side of road.
10. Let wind row aerate over night. Go Green With
Bio-Enzymes

54. Application Notes:- Day 3:
1. Moisten wind row soil with water truck spray bar.
2. Moisten soil in front of grader.
3. Grader blade 2” lifts/layer from wind row.
4. Sheep’s foot compactor follow grader blade.
5. Drum Compactor with vibrator on follows Sheep’s foot compactor.
6. Repeat #1 through #4 for 3/4 of expected depth or wind row.
7. Continue to moisten wind row.
8. Continue to use grader to blade 2” lifts.
9. Continue to use Drum compactor with vibrator off.
10. Repeat #6 - #8 for last 1/4 of expected depth.
11. Moisten surface in front of Pneumatic roller. Go Green With
Bio-Enzymes
12. Use Pneumatic compaction for smoothing final finish.
Allow to set for 3 – 5 Days with no traffic use. Traffic use too soon will cause rutting.

55. Using Enzymes in soil stabilization
 Terms Used in Road Construction

56. Terms Used in Road Construction

57. The Challenge faced when dealing with site
Engineers:
 Existing pavement design methods cater to relatively high
volumes of traffic with damaging effect quantified in terms
of ESA (equivalent standard axles). In contrast, main
factors controlling deterioration of LVRs (Low Volume
Roads) are dominated by the local road environment and
details of design (drainage), construction and maintenance
practice.
 Conventional specs apply to “ideal” materials
 Standard methods of test do not always give a true
assessment of performance of local materials
Go Green With
Bio-Enzymes

58. Primary Problem faced:
 “ The art of the roads engineer consists for a good part in
utilizing specifications that will make possible the use of
materials he finds in the vicinity of the road works.
Unfortunately, force of habit, inadequate specifications
and lack of initiative have suppressed the use of local
materials and innovative construction technologies”
 Consider materials’ “fitness for purpose”
 Make specification fit materials rather than materials fit
specification (“resource based” specs)
Go Green With
Bio-Enzymes

59. Pavement Performance:

60. Selection of material for construction:
 Material strength derived from combination of:
- Cohesive effects
- Soil suction
- Physio-chemical (stab) forces
- Inter-particle friction
 Material selection influenced by:
- Traffic loading
- Environment
- Material properties (plastic mod)
- Pavement configuration
Go Green With
Bio-Enzymes

61. Wide variety of chemical additives available
including:
 Wetting agents to improve compaction
 Hygroscopic salts (e.g. calcium, magnesium or sodium
chlorides)
 Natural polymers (e.g. ligno sulphonates)
 Synthetic polymer emulsions (e.g. acrylates)
 Modified waxes
 Sulphonated oils
 Biological enzymes
Go Green With
Bio-Enzymes

62. CBR versus stiffness

63. Stiffness versus density

64. Compaction/density/permeability

65. Dry density vs Permeability:

66. Dry density vs Stiffness:

67. Benefits of “Compaction to Refusal”

68. Effect of Surface Deflection on Seal Life:

69. Characteristics of Compaction equipment:

70. Road cross
section on
builtup

73. Primary Certification:

76. Field trail of BoroŹyme¦R DZ-1X on Black cotton soil as
a foundation for Road check
DCP Instrument
 The final layer was allowed to cure
for 7 days, after which a 7 day CBR
test was conducted using DCP
method.
 Determination of field CBR by
dynamic cone penetration test at
Shahol-Kim road
Go Green With
Bio-Enzymes

77. Resuts:
Shahol-Kim Road BoroŹyme¦R-
DZ-1X soil stabilization enzymes
treatment trials
Control (No Enzyme treatment)
parallel to
Enzyme treatment,
subject to same rolling
treatments as those in Enzyme
treatment
Go Green With
Bio-Enzymes

78. Expected Shift in your Soil CBR:-
100
90
80
70
60
50
40
30
20
10
0
0 5 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40
Corresponding % CBR of soil Expected new % CBR after DZ-1X Treatment
Power (Corresponding % CBR of soil) Power (Expected new % CBR after DZ-1X Treatment)

80. Sub-base design chart as per IRC-37

81. The Economics of using BOROŹYME¦R – DZ-1X:
 Road stabilization with BOROŹYME¦R – DZ-1X
offers significant economic alternatives to standard
road designs.
 Note that design strength has been maintained
while costs have been significantly reduced.
Go Green With
Bio-Enzymes

82. The Economics of using BOROŹYME¦R – DZ-1X:
Enamel seal
coat
Grit asphalt
mix
Rubble-soil
mix Box-Cut
required
CONVENTIONAL METHOD:
STRUCTURAL STRENGHT IS
MAINTAINED AT100% COST

83. Alternative# 1
 Maintains the standard
design strength while
reducing or eliminating costly
materials through
stabilization of local materials BOROŹYME¦R – DZ-1X
or economical material Box-Cut TREATED SUB-
BASE, WITH SAME
Not required
blends for typical cost DEPTH OF STANDARD
SUB-BASE
reductions of 20% - 30%.
Laboratory verification of soil
blends can be performed ENZYME TREATMENT:
STRUCTURAL STRENGHT IS MAINTAINED
before project execution REDUCING THE COST BY 20-30%

84. Alternative# 2
 Improves the standard design through
stabilization of available materials
blended to optimize gradation and
plasticity. The result is a maximum 30% DEPTH INCREASE OF SUB-BASE
increase in stabilized strength. Note TREATED WITH BOROŹYME¦R – DZ-1X
that savings of 40% are possible by
thinking outside of the standard design
and road materials. Cost comparisons No Digging
to matching structural upgrades using No Box-Cut
traditional materials and designs No foreign
soil required.
generally favor stabilization.
 These same alternatives can be used ENZYME TREATMENT:
for other surface coatings such as STRENGHT IS MAINTAINED
RCC, or Tar based seal coats. REDUCING THE COST BY 40%

85. Go Green With
Bio-Enzymes

86. PROJECT COLLABORATORS
Go Green With
Bio-Enzymes
www.boroninnovations.com
contact@boroninnovations.com
Skype: boroninnovations
+91-990-451-4703
Thank You and have a pleasant GREEN-DAY ahead..!!