4. www.neuroservice.com
WHO WE ARE
Core business activity
A Contract Research Organization (CRO)
Testing the effect of ANY COMPOUNDS
on Central Nervous System tissues
5. www.neuroservice.com
WHAT WE DO
Core business activity
IN VITRO TESTS
UNDER PHYSIOLOGICAL CONDITIONS
In rodent (or human) acute brain slices
In rodent spinal cord slices
In rodent cultured neurons
7. www.neuroservice.com
WHAT WE PROPOSE FOR DRUG DISCOVERY
Your remote laboratory
Hits
Leads
Advanced
candidate
IND
LEAD
SELECTION &
OPTIMIZATION
MECHANISM
OF ACTION
SAFETY &
TOXICOLOGY
PROFILING
CLINICAL RESEARCH
R&DPRÉ-CLINICAL
DEVELOPMENT
8. www.neuroservice.com
WHO WE ARE
Key features
Launched in Aix-en-Provence in 2006
Portfolio of 150 studies
60 worldwide clients (Pfizer, Roche, CHDI…)
35 people – 2 laboratories
Capital: 520 000€
Income 2014 : 2,7 M€
13. www.neuroservice.com
OUR TECHNIQUES: A COMPREHENSIVE COMBINATION
Different structures and protocols
MATERIAL STRUCTURE PROTOCOL
Input/Output Properties
Paired-Pulse Protocol
Basal Synaptic Transmission
Long-Term Potentiation
Long-Term Depression
Short-Term Potentiation
Firing Activity
Dose-Response Protocol
Spontaneous Postsynaptic Current
Agonist Evoked Postsynaptic Current
Pharmacology of Intracellular Pathways
Hippocampus
Cortex
Cerebellum
Spinal Cord with or without root
Ventral Tegmental Area
Striatum
Substantia Nigra
Locus Coeruleus
Periaqueductal Gray Matter
Dorsal Raphe
Amygdala
Thalamus
Dorsal Vagal Nucleus
i
In vitro
rodent slices
14. www.neuroservice.com
OUR TECHNIQUES: THE IDEAL COMBINATION
In vitro evaluation of compounds under the most physiologically relevant conditions
Robust and reproducible high quality data
Reducing animal and compound use
Exceptional macroscopic view of neuronal
networks
Ability to record multiple evoked-responses in
parallel within a single slice
Mid-throughput production capabilities
Refined investigation of compounds’ effect on
single proteins/receptors
Large catalog of CNS structures and neuronal
cell types to address effects on specific neurons,
neurotransmitter systems or receptors
NEURONAL NETWORK SINGLE NEURON
PATCH-CLAMPMEA (Multi-Electrode Arrays))
16. www.neuroservice.com
OUR FOCUS AREA
LEAD
SELECTION &
OPTIMIZATION
MECHANISM
OF ACTION
SAFETY &
TOXICOLOGY
PROFILING
SOLUTIONS
FOR CNS
DRUG
DISCOVERY
SOLUTIONS FOR
SAFETY:
Testing the effect of any
type of drug, molecule…
on CNS and investigate its
mechanism of action
18. www.neuroservice.com
SOLUTIONS FOR DRUG DISCOVERY
Example with MEA technique
ACCURATE PHARMACOLOGY
CHOLINERGIC RECEPTOR-MEDIATED INCREASE
OF CA1 PYRAMIDAL NEURONS FIRING
CARBACHOL &
PIRENZEPINE
M1 MUSCARINIC
ACETYLCHOLINE RECEPTORS
A B
CPirenzepineCarbachol
19. www.neuroservice.com
EXAMPLE WITH MEA TECHNIQUE
Tg Rodent Phenotyping
ELECTROPHYSIOLOGY IN BRAIN SLICES FROM
GENETICALLY ENGINEERED MICE:
Mice deficit (or gain) determination
Physiological characterizations
Drug evaluation in a disease model
FASTER
2 weeks
LESS ANIMALS REQUIRED
10 WT / 10 Tg
… in comparison with behavioural tests
LESS COMPOUNDS
REQUIRED
20. www.neuroservice.com
SOLUTIONS FOR DRUG DISCOVERY
Example with Patch-Clamp
CHOLINERGIC RECEPTOR-MEDIATED INCREASE
OF CA1 PYRAMIDAL NEURONS FIRING
CARBACHOL &
PIRENZEPINE
EXPERIMENT
Miniature inhibitory postsynaptic currents (mIPSCs) in
lamina II of adult spinal cord mice
DATA
mIPSC frequency, amplitude distribution and detailed
analysis of the average mIPSC waveform
(including amplitude, rise time, decay time)
100 pA
100 s
Strychnine 1 µM
Rat, p15, lamina X
TTX, 0.5 µM + ORG 24598, 10 µM, 35°C
dorsal
ventral
II
III
10x
21. www.neuroservice.com
SOLUTIONS FOR DRUG DISCOVERY
Example with Patch-Clamp
PHARMACOLOGICAL CHARACTERIZATION OF SODIUM CURRENTS IN DRG
TTXs and TTXr sodium current
n=6
TTXs sodium current
n=6
TTXr sodium current
n=3
Mice DRG (12 weeks) Rat DRG (12 weeks)
Inactivation Inactivation Inactivation
22. www.neuroservice.com
Human tissue management
Protocol design
Patch Clamp recording
HUMAN BRAIN SLICE RECORDINGS
A partnership customized to client needs
EXPERT INTERFACE
WITH CLIENT
REMOTE LABORATORY
Customer Relationship Management
Quotation & Contractual Paperwork
Contract and Project Management
Raw data validation & Final report
Clients
23. www.neuroservice.com
HUMAN BRAIN SLICE RECORDINGS
STRUCTURE MATERIAL PROTOCOLS
Neurons passive membrane properties
AMPA/kainate “minis” or evoked-currents
NMDA evoked-currents
GABAA “minis” or evoked-currents
…
Small areas of
cortical brain
tissue
Neurosurgical resections from adult
patients
For purely medical reasons (informed
consent given by patients)
Surgery approved by independent Board
of Physicians
All is supervised by the Ethical
Committee from the University of
Freiburg
(in accordance with both national and international
guidelines)
26. www.neuroservice.com
OUR SOLUTIONS
Neuroservice: your remote laboratory
Outsourced Assays R&D Customized Solutions Packages
DRIVE
OUR RESSOURCES
CUSTOMIZE
OUR ASSAYS
CHOOSE
A READY TO USE
SOLUTION
Highly experienced team in
neurophysiology,
neuropharmacology
and neurotoxicology
Portfolio of 150 studies
• High quality data
•Short turnaround time
•Mid-throughput production
capabilities
4 packages:
34. www.neuroservice.com
OUR COMMITMENTS
QUALITY ROBUST AND REPRODUCIBLE DATA
SHORT TURNAROUND 100 VALIDATED SLICES OR 40 NEURONS / WEEK
EXPERTISE INTERACTIVE AND EXPERT SUPPORT
FLEXIBILITY STEP-BY-STEP STUDY PLANS
ETHICS MINIMIZED ANIMAL USE
36. www.neuroservice.com
YOUR INTERLOCUTORS
BRUNO BUISSON, PhD
Founder
General manager &
Chief scientist
SCIENTIFIC TEAM
Based in JapanBased in USA
TATSUO MIYAUCHI, PhD
Scientific liaison
JEFFREY SPROUSE, PhD
Scientific liaison
OLIVIER TOURY
Co-Founder
Deputy general manager &
Chief of business
development
MAI SAITO
Asia Business Developer
LINDA HOUAICHI
USA & Europe Business
Developer
ESTHER STEIDL, MsSc
Co-founder
Head of the Multi-Electrode
Array laboratory
BOGDAN SAVA, PhD
Head of the Patch-Clamp
laboratory
BUSINESS TEAM
37. www.neuroservice.comYour partner for hands-on CNS electrophysiology
Domaine de St Hilaire
595, rue Pierre CS 30531
13 593 Aix-en-Provence
Cedex 3
FRANCE
Tel : +33 (0)442 991 220
contact@neuroservice.com
www.neuroservice.com
