Overall, testing cfDNA has four distinct advantages over conventional biopsies, being:   Cost-effective approach; Simplified sample collection procedures; Reduced impact to the patient and; Easily analyzed.

1. HORIZON DIAGNOSTICS
Cell Free DNA comes to the Clinic
1
Joe Whittaker
Research Use Only

2. 2
Figure - Squamous cell carcinoma of
lung (left half of image) replacing
normal healthy lung tissue (right
side)
The importance of NSCLC therapies
is paramount in the fight against
cancer. Lung cancer, in particular, is
the leading cause of cancer-related
deaths worldwide with over 1.1
million patients and 1.2 million new
cases of lung cancer diagnosed
every year worldwide (1).
1. Jemal, A, et al., et al. Cancer Statistics. A Cancer Journal for Clinicians. 57, 2007, Vol. 1.
Research Use Only
Non-Small Cell Lung Cancer

3. Overall, testing cfDNA has four distinct advantages over
conventional biopsies, being:
• Cost-effective approach;
• Simplified sample collection procedures;
• Reduced impact to the patient and;
• Easily analyzed.
3
Crucially, the combination of these advantages allows for repeat routine testing of the
patient through diagnosis to efficacy of treatment.
Research Use Only
Advantages

4. 4
“…large variance in concordance rates...different methods of extraction, detection,
run conditions, the size and yield of the DNA fragments”
Reference Materials
Research Use Only
However…Variability Arises

5. 5
0
5
10
15
20
25
30
35
40
45
Positive:Positive Positive:Negative Negative:Positive Negative:Negative
PercentageRespondents(%)
Tumor:cfDNA Result
Figure – adapted from Goto et al., showing the variability between EGFR detection of tumor DNA and cfDNA
Research Use Only
Detected Results Using Either Tumor or cfDNA

6. 6
Coming soon...
Contact Details
j.whittaker@horizondiscovery.com
Research Use Only
cfDNA Reference Standards