Drosophila como modelo

J
Jimena SierraltaAssociate Professor
Drosophila as model for neural development Jimena Sierralta ICBM, Universidad de Chile and CENI
Why Drosophila? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Genetic ,[object Object],[object Object],[object Object],Thomas Hunt Morgan: 1866-1945.  1933 Nobel Prize in Medicine and Physiology.
Drosophila ,[object Object],[object Object]
 
Life cycle AT 25  �ºC, 10 days
 
Embryogenesis
Embryonic Neural development
 
Neuroblasts Map
 
Pathfinding mutants
Dlg S97 expression in the embryo CNS I S97N FasIII H G MG S97N  HB9 Eve S97N MG (  -gal) M S97N   Repo gcm (  -gal) K L S97N   Eagle Elav S97N  Eagle
* * E F G H Neuroblasto mitótico Dlg (PDZs) Baz Pins Mir Pros Lgl * A B C D IP  Pins IP Pins IP  Pros PDZ   Pros IP   Mir
P elements in Drosophila
Transgenesis in flies by P element insertions.
Sistema UAS-GAL4 ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
UAS-GAL4 system
Enhancer Trap lines
UAS-EGFP DlgS97 X pros-Gal4 Fas II 22C10   elav 22C10   elav UAS-S97N-EGFP X elav-Gal4
Use of enhancer trap lines
In vivo studies
In vivo experiments
 
Use of transgenic as mutant markers
Neuromuscular Junction
 
Efectos asociados a la pérdida de expresión de las variantes con dominio S97N en UNM S97N FasII HRP Merge Control RNAi postsináptico dlgXI-2 dlg 51-1
Efectos asociados a la pérdida de expresión de las variantes con dominio S97N en UNM ,[object Object],S97N Scrib HRP Merge RNAi postsináptico dlgXI-2 dlg 51-1
HRP Merge 22C10 S97N NC82 Merge S97N HRP:   marcador presináptico. 22C10:   marcador axonal. S97N :  Las variantes con dominio S97N se expresan en los botones sinápticos, con escasa presencia a nivel presináptico. NC82:   marcador de zonas activas.
Efectos diferenciales asociados a la sobreexpresión de DlgS97 y DlgA en la UNM ,[object Object],Sobreexpresión de DlgS97  Sobreexpresión de DlgA ,[object Object]
Photoreceptors Distal Proximal
Photoreceptors development
 
Fruitless specifies sexually dimorphic neural circuitry
Females turn into males by the expression of a gene
MARCM
Axon guidance in adults
Protein dynamics
 
 
RNAi screens in cells
1 sur 43

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Drosophila como modelo