5. 1st Generation of Bioethanol
Sugars
extract ferment
ethanol
sugarcane
BRAZIL
(sucrose)
Sugars
Hydrolyze
(enzymes)
ferment
ethanol
USA
(starch)
Cosgrove; 2005
6. Types of Biofuel
Solid animal wastes and agricultural residues can be
used as sources of energy by direct burning (primitive way)
Liquid Bioethanol C2H5OH ( fermentation of
sugar)
Biodiesel (by saturation of vegetable
oils)
Gas e.g. methane and biogas derived from organic
wastes by anaerobic digestion
Organic wastes Heat energy
Direct burning
10. Composition of Lignocellulose
Cellulose
Hemicelluloses
Lignin
Ash
Extractives
Cellulose
Hemicellulose
(both 5 and 6 carbon sugars)
(need modified microbe to
convert to ethanol)
Ash
Extractives
Lignin (phenols)
(6 carbon sugars)
Chapple, 2006; Ladisch, 1979, 2006
11. Pretreatment
break down the
shield formed by
lignin and
hemicellulose
Open the fiber
structure
reduce the degree
of polymerization
of cellulose.
Source: Overview of biomass pretreatment
for cellulosic ethanol production; 2009
12. Pretreatment has been viewed as one of the most
expensive processing steps within the conversion of
biomass to fermentable sugar
Pretreatment methods maybe: physical, chemical or
biological
Biological:
Adv. : no chemicals, no energy requirements, mild
environmental conditions
Disadv.: slow, the activity of the microorganisms maybe
not specific to lignin only!
13. Pretreated Lignocellulose
What is “Pretreated Biomass”?
increased surface area,
solubilization of cellulose,
redistribution of cellulose and lignin
Cellulose 35-50%
Hemicellulose 20- 35%
Lignin 5-30%
Microbial cellulose utilization fundamental and biotechnology; 2002
15. Microbial Enzyme system:
Substrate cellulose + hemicellulose
Enzymes:
endoglucanases: cut at random internal sites
along the cellulose/hemicellulose chain
exoglucanases: act at reducing and nonreducing
ends
beta-glucosidase: break betaglucoside bond to
form glucose
16. Enzyme system
Cellulose Oligosaccharides (<10)
Endogluconase
Cellobiose
+ glucose
glucose
Exoglucanase
Beta-glucosidase
Microbial cellulose utilization fundamental and biotechnology; 2002
17. Lignocellulosic Activities of
Actinomycetes
According to Lynd et al (2002) there is a considerable
concentration of cellulytic capabilities among
Actinomyceltales.
Actinomycetes are well known for their ability to
decompose complex molecules, particularly
lignocellulose components
Micromonospora spp and Strptomyces spp are well
known for their decomposition ability on Biomass
18. Actinomycetes and cellulytic
activities
Growth TempSpeices
mesophilicM. chalcea
mesophilicS. roseflavus
MesophilicS. reticuli
ThermophilicThermobifidia fusca
mesophilicKibdelosporanguim Philippinenses
Most of actinomycete species can be isolated from both soil and
water.
19. Bioprocessing of cellulosic Biomass
Steps (mediated events):
1) Cellulase production
2) Hydrolysis of
cellulose/hemicellulose
3) Fermentation of cellulose
hydrolysis products e.g.
glucose
4) Fermentation of
hemicellulose hydrolysis
products other than
glucose e.g. xylose
biomass
fuel
Microbial cellulose utilization fundamental and biotechnology; 2002
20. Bioprocessing of cellulosic Biomass
This diagram shows the capability of consolidation or separation of
mediate events (steps) of bioprocessing of Biomass
Source: Microbial cellulose utilization fundamental and biotechnology; 2002
• SHF: Separated
Hydrolysis and
Fermentation
• SSF: Simultaneous
Saccharification and
Fermentation
• SSCF: Simultaneous
Saccharification and
Cofementation
• CBP: Consolidate
Bioprocessing
21. Consolidated Bioprocessing CBP
In which all bioprocessing steps are combined together
as one process
Biomass processing technology has exhibited a trend
toward increasing consolidation over time
Advantages Efficiency + Economically effective
CBP organisms:
Single organism
Community of organisms( symbiotic consortium)
(which is more efficient???)
22. Symbiotic Consortium
A community of organisms
i.e 2 or more organisms living in association
Symbiosis may be : mutualism, commensalism, o
antagonism
Types:
Natural consortuim
Engineered consortuim
Genetically
Recombined natural capabilities
i.e. ecological approaches
23. Natural Consortium
The main problem doesn’t accumulate high levels
of biofuel why?
Biofuel molecules are molecules of energy
Biofuels represents an a pportunity for a new consortia
member (organism) to exploit
Natural consortia tend to thermodynamically free
energy of molecules till the lowest level
Be overcome by engineering consortia
25. Sequential utilization
2 oranisms M1 and M2
The fuel molecule (F1) is
considered a waste product of
M1. However, it is degraded by
M2 as source of energy e.g.
commensalism
No accumlation of fuel
molecules
26. Co-utilization
M1 & M2 are competing to
utilize the substrate , producing
fuel molecules
Competitive symbiosis i.e.
controlled by inhibitors
/activatiors
Fuel considered waste product
of both organsims
There is accumulation of fuel
27. Substrate transformation
M1 acts on substrate converting
it to a form that can be utilized
by M2
e.g. pretreatment of
lignocellulosic material
mutualsim
28. Product transformation
M1 produces fuel products as
waste product
M2 act on fuel to convert it into
an alternative fuel
Look like sequential utilization.
However, the fuel molecules are
converted to alternative fuel ,
not completely utilized