10 Beta House,
CAREER OBJECTIVE & SUMMARY:
“I’m currently looking for a suitable Research Assistantposition/ lab technician in biotechnological/
microbiological industry/cancer research centre to utilize knowledge and experience in cell and tissue
engineering,cancer research,and micro biologywith a reputable organisation.”
Expertise in microbiological research control,developmentand culture of cells in-vitro environment.
Knowledge ofgood laboratory practices and sound scientific microbiological,cell tissue engineering and
Having excellentresearch potential and an ability to actively contribute to the research projects goals as
well as a proven publication track record and a good standard ofwritten English.
Able to interactwith all researchers in a constructive, creative and professional manner during the
processing,storing and logging process.
KEY TECHNICAL SKILLS:
Molecular: Reverse Transcriptase PCR ,Polymer chain reaction, Western blotting,ELISA, cell counting
Cell culture: Agglutination test,Single cell isolation, cell maintenance,cell passage, Trypsinise cells,cell
passaging,freezing cells,Thawing and recovering,cells transportation.
Immunology: Cell staining, Electrophoresis.
Microbiology: Agar diffusion test,ATP Test, Blood culture,Citrate test, endospore stain,Industrial fermentation,
Live cell imaging,Microbiological culture,Microscopy, Streaking, oxidase test,Sterilization, Replica planting,
Ziehl–Neelsen stain,Viability assay.
Equipment Used: Cell culture hood (i.e., laminar-flow hood or biosafetycabinet),Incubator (humid CO2 incubator
recommended),Water bath, Centrifuge,Refrigerator and freezer (–20°C),Cell counter (e.g., Countess®
Automated Cell Counter or haemocytometer),Inverted microscope,Liquid nitrogen (N2) freezer or cryostorage
container,Sterilizer, pH meter,Flow cytometer, Cell culture vessels (e.g.,flasks,Petri dishes,roller bottles,multi-
well plates),Pipettes and pipettors,MR5000 microplate reader,flow cytometry
In Academic Research experienced in
-Undertaking research,Survey, Clinical trials and techniques
-Data collecting and Data validation by using graph-pad prism
Soft Skills and Computer Knowledge
Excellent written and verbal communication skills.
Hardworking and Positive attitude
Ability to Work in/as a Team,also manage a team.
Decision making and problem solving skills,able to delegate work when required.
Good working knowledge of MS Word , Excel ,PowerPoint and Misc IT skills as partofwork experience
(prepare presentations, spread-sheets and reports)
TK Maxx Retail (Aug 2014 –Nov 2015)
Job Profile: Sales Assistant
Roles & Responsibilities:-
To maximise sales byoffering excellent customer service and driving retail standards
Identifying customers needs and responding to them quicklyand efficiently
Operating a computerised till & Replenishing stock
Actively supporting and communicating with colleagues and managers
Developing working relationships with team leaders,store managers and other associates
Applying basic merchandising skills
Processing deliveries and replenishing stock on stands
Acknowledging all customers positivelyand professionally
Demonstrating customer care and high levels of customer service both on the sales floor and till points
Supporting colleagues with their work and getting involved in day-to-day activities
Gained experience in team work, till work, retail experience,soft skills,dealing with customer problems.
Zydus ResearchCentre (Sept 2012–March2014)
Job Profile: Clinical Research Assistant
Roles & Responsibilities:-
Prepare media, specimen, making solution, pouring agar plate
Culture and Aseptic techniques,steak plating,growing E-coli and culture them
Microbial culture techniques, staining techniques, samples testing techniques
Conducted data collection, source data verification and generated the quality control reports for the
Data sheets generated byClinical Data Managementfrom the source documents and checked reports for
Ensured drug supply, storage and packaging conditions as per the manufacturer requirements and
quantity as per the study and regulations requirement,receiptof drug from sponsor for all studies.Check
master drug accountabilitylog,dispensing studydrug to trial subjects.
Generated quality control reports for the ongoing studyactivities, Clinical facility, Drug store,
Conducting studies according to the ICH-GCP, protocol, and SOP’s requirement.
Verified maintenance and calibration ofequipment,and ensured proper logistics oflaboratorycollections,
processing,storage and shipmentofspecimens.
Managed and Reviewed Study documents (protocol,Informed consent,case reportforms,source
Attended meetings held for the study flow improvementand queryresolution within Clinical Research
Departmentand Quality Assurance Department.
Guy Hilton ResearchCentre (Jan 2012 – Aug 2012)
Stoke-On-Trent , Staffordshire
Job Profile: Laboratory Technician
Roles & Responsibilities:-
Regular maintenance and culture of primary and mammaliancells includes ovarian cancer cell lines.
Growing cells in RPMI and DMEM, culture them in FCS, Penstrep,Glutamine.
Trypsinize the cells with trypsin and EDTA and Passage cells followed byincubation.
Prepare 10X stock of drugs (statins) with growth medium and add specific portion to each cell lines.
Conducted a continuous experimenton 8 different cell lines with 6 different statins and mevalonate.
Arranged a comparative studybetween simvastatin and simvastatin acid.
Apoptosis with Caspase Glo after treated with Simvastatin.
Collection ofcells by centrifugation and count in Haemocytometer.
Preparing specimens and samples and Ensuring the laboratoryis well-stocked and resourced
Following and ensuring strictsafetyprocedures and safetychecks.
Supervise and train undergraduate students on skills in how to treat ovarian cancer cells with various
statins and evaluate drug potency.
AssistPhD students in writing scientific manuscripts and conductscientific protocols.
Helping professor and other fellow studentin terms of research paper publication.
First Advantage, Bangalore India (June 2008 – Jan 2011) Verification Executive
I was responsible for the educational and employment background verification for candidates who
applied for jobs in a UK banking sector such as RBS, Barclays etc. The company was a UK based
company and I managed a team of 20 people which has given me good leadership and management
skills. I later moved to the ‘Quality Analyst’ team where we had to verify all the relevant details of the
candidates. This aspect of the work required thoroughness, attention to detail and methodical
thinking all skills that I still retain. I also learned a lot about the UK education system through the
McAfee Bangalore India (March 2007 - May 2008) Technical and customer support
Provide thorough supportand problem resolution for customers call from UK,USA, AUSTRALIA, CANADA and
Accomplished with McAfee anti-virus
Maintain composure and patience in face of difficultcustomer situation
Train new employee and explain difficult protocols clearlyand efficiently
Achieved 1/ 2 ranking in monthlymetrics including call duration,number ofcalls,and customer satisfaction rating
Proficient trouble shooter, Exceptional telephone etiquette, patients and diligent, vast technical
knowledge, diverse PC knowledge, LAN expertise.
Borrowing Hospital, Bangalore India (July 2006 – Feb 2007) Receptionist
I was responsible for attending calls, scheduling doctor’s appointments, managing out-patients and also
helping patients with any other issues they had.
Duties will include co-ordinating the MDT meetings and organising all the documentation
Had to effectively communicate with clinical and clerical staff across all internal and external
EDUCATION & ACADEMIC QUALIFICATIONS:
(2011-2012) Master in Cell and Tissue engineering from Keele University, Staffordshire
Cell and Tissue engineering subjectwas quite vastwhere I could get an opportunity to learn a lot aboutBioreactor
& Growth Environment,Biomechanics,Cell and Tissue Engineering,Biomaterials,Nanomagnetics in
Nanomedicine,Cell Biomechanics,Stem Cell Therapy, Biosensors and Molecular Techniques.
Dissertation: Have done my Master Project on Ovarian cancer: The main aim ofthe project was which drug is
mostpotentto kill the Ovarian Cancer Cell. I’ve worked on various statins (Simvastatin,Rosuvastatin,Atrovastatin,
Simvastatin Acid, Lovastatin, Fluvastatin) and got a very promising resultoutif , which concluded thatthe
Simvastatin is the mostpotentto kill Ovarian Cancer Cell.
(2002–2005) BSc Microbiology , Bangalore University, India
Had worked in microbiologylaboratory and gained practical knowledge in cell culture,media preparation,growing
bacteria and various viral diseases.Cultured urine,blood and sputum. wentfor an industrial tour and visited
institutes and observed the research work including Indian Institute ofHorticulture research,Bangalore Diary,
National Tuberculosis Institute,National Dairyresearch Institute,Central food technological research institute.
CONFERENCE & PUBLICATION:
Attended Joseph sule suso seminar on lung cancer at Keele University
Geoff tansleyseminar on cardiac devices and blood flow and mechanics,On shear flow,
Seminar on statistics.
Attended British gynaecological cancer societyconference in London and Ireland.
Preclinical evaluation of statins as a treatmentfor ovarian cancer,Robinson E,Nandi M, Wilkinson LL,
Arrowsmith DM, Curtis AD, Richardson A.
Gynecol Oncol. 2013 May;129(2):417-24. doi:10.1016/j.ygyno.2013.02.003.
I also have my own blog site http://biotechisamazing.blogspot.in/
HOBBIES AND INTERESTS:
I enjoy cycling, tennis,snooker,darts and manyother sports;I like a variety of different music.
I like traveling, and cooking multi-cuisine food.I also like to watch a variety of differentfilms,and go to the cinema