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Gene Cloning & Creating DNA Libraries
Gene Cloning ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Cloning - a definition ,[object Object],[object Object],[object Object]
What is DNA cloning?   ,[object Object],[object Object]
Whole organisms are cloned too, but differently
Why Clone DNA? ,[object Object],[object Object],[object Object],[object Object],[object Object]
How is DNA cloned? ,[object Object],[object Object],[object Object],Blood sample DNA Restriction enzymes
The action of a  restriction enzyme ,  EcoR I Note: EcoR I  gives a ‘sticky’ end
DNA Cloning, II ,[object Object],[object Object]
DNA cloning, III ,[object Object],[object Object]
DNA Cloning IV ,[object Object],[object Object]
Antibiotic Resistance ,[object Object],[object Object],[object Object]
Cloning V ,[object Object],[object Object],[object Object]
Gene Libraries ,[object Object],[object Object]
Screening I ,[object Object],[object Object],[object Object]
Screening II ,[object Object]
Screening III ,[object Object],[object Object]
cDNA I ,[object Object],[object Object]
A simplified diagram of transcription in eukaryotes hnRNA = ‘heterogenous nuclear’RNA in the process of being cut and spliced into messenger RNA
cDNA II ,[object Object],[object Object]
cDNA III ,[object Object],[object Object],[object Object]
Considerations ,[object Object]
Considerations, ctd . ,[object Object],[object Object],[object Object]
These points and others require research and  informed debate- What are  Your  thoughts? What legislation has been passed here in New Zealand? Who controls work in genetic manipulation?
References Kreuzer, H., Massey, A.,2001,  Recombinant DNA & Biotechnology , ASM Press, Washington Turner, P.C., et al, 1997,  Instant Notes n MolecularBiology , Bios, Oxford www.agbiosafety.unl.edu/education/clone.htm http://avery.rutgers.edu/WSSP/Student   Scholars/Session12/Session12html http://www.pssc.ttu.edu/pss3421/gene%20cloning%20Strategies.htm http://www.uic.edu/classes/phar/phar331/lecture7 http://www.biology.arizona.edu
Picture Credits Cc the cat, slide 5 courtesy of Texas A & M University,  College of Veterinary Medicine Photos on slide 5, 7,12 & 17, L. Macdonald 2003 Graphics on slides 4, 8 &19, V. Ward, University of Auckland DNA Hybridization, slide 15, courtesy of Texas Tech University DNA cloning & screening, slides 10 & 14, courtesy of The University of Arizona
Acknowledgements Thanks go to Craig Millar and Andrew Dodd, School of Biological Science,  University of Auckland Compiled by  Linda Macdonald For NCEA Biology A.S. 3.6  While on a New Zealand Royal Society Science, Mathematics & Technology Teacher Fellowship

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Gene cloning

  • 1. Gene Cloning & Creating DNA Libraries
  • 2.
  • 3.
  • 4.
  • 5. Whole organisms are cloned too, but differently
  • 6.
  • 7.
  • 8. The action of a restriction enzyme , EcoR I Note: EcoR I gives a ‘sticky’ end
  • 9.
  • 10.
  • 11.
  • 12.
  • 13.
  • 14.
  • 15.
  • 16.
  • 17.
  • 18.
  • 19. A simplified diagram of transcription in eukaryotes hnRNA = ‘heterogenous nuclear’RNA in the process of being cut and spliced into messenger RNA
  • 20.
  • 21.
  • 22.
  • 23.
  • 24. These points and others require research and informed debate- What are Your thoughts? What legislation has been passed here in New Zealand? Who controls work in genetic manipulation?
  • 25. References Kreuzer, H., Massey, A.,2001, Recombinant DNA & Biotechnology , ASM Press, Washington Turner, P.C., et al, 1997, Instant Notes n MolecularBiology , Bios, Oxford www.agbiosafety.unl.edu/education/clone.htm http://avery.rutgers.edu/WSSP/Student Scholars/Session12/Session12html http://www.pssc.ttu.edu/pss3421/gene%20cloning%20Strategies.htm http://www.uic.edu/classes/phar/phar331/lecture7 http://www.biology.arizona.edu
  • 26. Picture Credits Cc the cat, slide 5 courtesy of Texas A & M University, College of Veterinary Medicine Photos on slide 5, 7,12 & 17, L. Macdonald 2003 Graphics on slides 4, 8 &19, V. Ward, University of Auckland DNA Hybridization, slide 15, courtesy of Texas Tech University DNA cloning & screening, slides 10 & 14, courtesy of The University of Arizona
  • 27. Acknowledgements Thanks go to Craig Millar and Andrew Dodd, School of Biological Science, University of Auckland Compiled by Linda Macdonald For NCEA Biology A.S. 3.6 While on a New Zealand Royal Society Science, Mathematics & Technology Teacher Fellowship