1. Matthew Paul Tugwell matthew.x.tugwell@gsk.com (Work) / mptugwel@ncsu.edu (Personal)
7409 Tynewind Drive 1-919-483-6902 (Work) / 1-919-612-9979 (Mobile)
Wake Forest, NC 27587
OBJECTIVE:
My goal is to obtain a position in a biomedical research facility to gain experience and to utilize my scientific knowledge
in a research environment.
EDUCATION:
B.S. in Biochemistry, North Carolina State University, Raleigh, NC. May 2013. GPA: 3.0
RELEVANT COURSEWORK:
Biochemistry, Organic Chemistry, Physical Chemistry, Genetics, Inorganic and Quantitative Chemistry, Statistics,
Biology, Microbiology, Math.
I have educational laboratory experience including:
 DNA: Extraction, Quantification, PCR, qPCR, agarose gel electrophoresis, Southern blotting, plasmid minipreps,
restriction endonuclease digestion, isolating DNA fragment inserts and ligation of inserts into plasmids.
 RNA: Extraction, Quantification, cDNA Synthesis (Reverse Transcription PCR) and Northern blotting.
 Protein: enzyme kinetics and activity, protein preparation and purification, fractionation, SDS-PAGE and ELISA.
 Quantitative Chemistry (Spectrophotometry, GC and HPLC).
ATTRIBUTES:
 Passion for math, science and molecular and structural biochemistry.
 Strong oral communication skills and technical writing skills.
 Understanding of Statistics and Power of Experimental Design
 Computer Software skills: Microsoft Word®, Microsoft Excel®, Microsoft PowerPoint®, Microsoft Outlook®
 DNA: Extraction, Quantification, PCR and qPCR
 RNA: Extraction, Quantification and cDNA Synthesis (Reverse Transcription PCR)
 Genotyping: SNP/Mutation Detection
 Differential Expression Analysis using qPCR
 Next Generation Sequencing Data Analysis (Differential Expression) Software: Array Studio (Omicsoft
Corporation)
 Quality Control and Troubleshooting of Next Generation Sequencing Platforms (Ion Torrent PGM)
 Automation of RNA Extraction and High Throughput Experimental Design
 Handling Hazardous Chemicals and Potentially Infectious Human Biological Samples
EXPERIENCE:
September 9th, 2013 – February 13th, 2015:
GlaxoSmithKline, Discovery Core Technologies and Capabilities, Platform Technology and Science, RTP, NC
DNA Sequencing Lab Agency Worker Position within GlaxoSmithKline’s Platform Technology Sciences Department
with Manager Ermias Woldu located in Research Triangle Park.
 Provide high quality sequencing data and analysis to support projects at multiple stages of pharmaceutical
development.
 Responsible for providing Sanger, STR fingerprinting (ABI 3730XL), Next Generation Sequencing (Ion Torrent
PGM) and qPCR (ABI 7900HT) data.
 Experience with robotic lab equipment (Biomek FX).
 Use of sequencing data analysis software for SNP/mutation detection, differential expression and human cell
identification (Sequencher, Array Studio and Genemapper).
 Proficient with Local Integrated Management Systems (Nautilus, Run Check and Genoportal).
 Provide project leaders with sequencing data reports summarizing results.
REFERENCES
Available upon request.