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©2010 Mourne Training Services,[object Object]
©2010 Mourne Training Services,[object Object],Dear Lab Tech Guy,,[object Object],I have often wondered if the high temperatures used when cleaning and drying volumetric glassware have any effect on the delivered volume. Can you shed any light on this? ,[object Object],Yours,,[object Object],Martin,[object Object],(Stockport, England),[object Object]
Dear Martin,,[object Object],Most modern laboratory glassware is made from Pyrex, a material which is resistant to irreversible volume changes. A study by  D.R.Burfield* showed that exposing volumetric flasks and pipettes to 320⁰C, either continuously or thermally cycled, does not significantly affect the calibration, the conclusion being that "oven temperatures in the range of 110-150⁰C should provide efficient drying of glassware with no risk of discernible volume changes, even after prolonged use, providing that Pyrex glass is the material of construction". ,[object Object],If you read the manufacturer’s instructions for your glassware then it is likely to warn against exposing to direct heat but will advise that use of automatic dishwashers and autoclaves at 121⁰C is acceptable.,[object Object],*	’Oven Drying of Volumetric Glassware’ D.R.Burfield and G.HefterJ.Chem.Ed. v.64 p.1054 (1987),[object Object], ,[object Object],©2010 Mourne Training Services,[object Object],Phew! Does it have to be so hot?,[object Object]
In fact, you don’t usually need to dry volumetric glassware since it can be rinsed with the appropriate solution or diluent at the time of use but you DO need to be very sure it is clean. Not only to prevent contamination but also to ensure that the correct volume is measured. Dirt, and especially grease, can distort the shape of the meniscus and also cause droplets of liquid to adhere to the vessel walls. Both seriously impair accuracy.,[object Object],My advice is to get your hands on a copy of the manufacturer’s recommendations for cleaning your volumetric glassware and follow the instructions provided. Also, make sure to clean glassware thoroughly for good measure.,[object Object],Yours volumetrically, ,[object Object],©2010 Mourne Training Services,[object Object],What’s he so happy about?,[object Object],He’s been like that ever since the  Prozac® analysis ,[object Object]
Brought to you by...,[object Object],www.mournetrainingservices.co.uk,[object Object],©2010 Mourne Training Services,[object Object]

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Lab Tech Guy on the Effect of Heating Volumetric Glassware

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